AIM To investigate the morphological andultrastructural changes in the human gastriccarcinoma cell line BGC-823 after being treatedwith tachyplesin.METHODS Tachyplesin was isolated from acidextracts of Chinese horsesh...AIM To investigate the morphological andultrastructural changes in the human gastriccarcinoma cell line BGC-823 after being treatedwith tachyplesin.METHODS Tachyplesin was isolated from acidextracts of Chinese horseshoe crab(Tachypleustridentatus)hemocytes.BGC-823 cells and thecells treated with 2.0mg/L tachyplesin wereexamined respectively under light microscope,scanning and transmission electron microscope.RESULTS BGC-823 cells had undergone therestorational alteration in morphology andultrastructure after tachyplesin treatment.Thechanges were as follows:the shape of cells wasunanimous,the volume enlarged and cellsturned to be flat and spread,the nucleo-cytoplasmic ratio lessened and nuclear shapebecame rather regular,the number of nucleolusreduced and its volume lessened,heter-chromatin decreased while euchromatinincreased in nucleus.In the cytoplasm,mitochondria grew in number with consistentstructure relatively,Golgi complex turned to betypical and well-developed,rough endoplasmicreticulum increased and polyribosomedecreased.The microvilli at cellular surfacewere rare and the filopodia reduced whilelamellipodia increased at the cell edge.CONCLUSION Tachyplesin could alter themalignant morphological and ultrastructuralcharacteristics of human gastric carcinoma cellseffectively and have a certain inducing differen-tiation effect on human gastric carcinoma cells.展开更多
Birds have specific habitat needs as a function of their life cycle and reproductive stage. Migrant shorebirds that may fly from the Arctic to the southern tip of South America have foraging and habitat requirements a...Birds have specific habitat needs as a function of their life cycle and reproductive stage. Migrant shorebirds that may fly from the Arctic to the southern tip of South America have foraging and habitat requirements at sites where they stop to refuel before continuing their migration north or south. Throughout the world, shorebirds mainly forage on mudflats at low tide. Red knots (Calidris canutus rufa) are threatened in the United States and elsewhere, and it is critical to determine factors that might contribute to their decline. This paper uses Delaware Bay as a case study to examine shorebird (and red knot) use of the intertidal habitat, and competing claims to habitats they require during their northward migration, as well as some of the key stakeholders that play a role in protecting red knots. Shorebirds are drawn to Delaware Bay to feed on the eggs of Horseshoe Crabs (Limulus polyphemus) that are concentrated at the high tide. But they also feed on the intertidal mudflat. We examined intertidal habitat use on 17 beaches in an extensive study in 2015, and 5 key beaches in 2016. Most of the beaches were longitudinal, but four were more complex, and were used extensively for resting as well as foraging;numbers there were higher than on the longitudinal beaches. On foraging beaches, some shorebirds were present on over 85% of the intertidal censuses, and red knots were present on over 48% of the intertidal censuses. Average numbers of red knots on the longitudinal beaches varied from 0 to 354 ± 116 when any shorebirds were present, but averaged up to 1184 ± 634 when knots were present in 2015. Some beaches in 2015 had no knots (a beach with long-term aquaculture). Tide, intertidal location, and beach (name) determined the number of knots (and all shorebirds). Numbers decreased with distance from the mean high tide line. The average number of knots present in the intertidal mudflats two hours before or after low tide when knots were present (e.g. no censuses with zeros) was 2040 (=maximum flock size, in 2015展开更多
基金the Natural Science Foundation of Fujian Province,No.C97015
文摘AIM To investigate the morphological andultrastructural changes in the human gastriccarcinoma cell line BGC-823 after being treatedwith tachyplesin.METHODS Tachyplesin was isolated from acidextracts of Chinese horseshoe crab(Tachypleustridentatus)hemocytes.BGC-823 cells and thecells treated with 2.0mg/L tachyplesin wereexamined respectively under light microscope,scanning and transmission electron microscope.RESULTS BGC-823 cells had undergone therestorational alteration in morphology andultrastructure after tachyplesin treatment.Thechanges were as follows:the shape of cells wasunanimous,the volume enlarged and cellsturned to be flat and spread,the nucleo-cytoplasmic ratio lessened and nuclear shapebecame rather regular,the number of nucleolusreduced and its volume lessened,heter-chromatin decreased while euchromatinincreased in nucleus.In the cytoplasm,mitochondria grew in number with consistentstructure relatively,Golgi complex turned to betypical and well-developed,rough endoplasmicreticulum increased and polyribosomedecreased.The microvilli at cellular surfacewere rare and the filopodia reduced whilelamellipodia increased at the cell edge.CONCLUSION Tachyplesin could alter themalignant morphological and ultrastructuralcharacteristics of human gastric carcinoma cellseffectively and have a certain inducing differen-tiation effect on human gastric carcinoma cells.
文摘Birds have specific habitat needs as a function of their life cycle and reproductive stage. Migrant shorebirds that may fly from the Arctic to the southern tip of South America have foraging and habitat requirements at sites where they stop to refuel before continuing their migration north or south. Throughout the world, shorebirds mainly forage on mudflats at low tide. Red knots (Calidris canutus rufa) are threatened in the United States and elsewhere, and it is critical to determine factors that might contribute to their decline. This paper uses Delaware Bay as a case study to examine shorebird (and red knot) use of the intertidal habitat, and competing claims to habitats they require during their northward migration, as well as some of the key stakeholders that play a role in protecting red knots. Shorebirds are drawn to Delaware Bay to feed on the eggs of Horseshoe Crabs (Limulus polyphemus) that are concentrated at the high tide. But they also feed on the intertidal mudflat. We examined intertidal habitat use on 17 beaches in an extensive study in 2015, and 5 key beaches in 2016. Most of the beaches were longitudinal, but four were more complex, and were used extensively for resting as well as foraging;numbers there were higher than on the longitudinal beaches. On foraging beaches, some shorebirds were present on over 85% of the intertidal censuses, and red knots were present on over 48% of the intertidal censuses. Average numbers of red knots on the longitudinal beaches varied from 0 to 354 ± 116 when any shorebirds were present, but averaged up to 1184 ± 634 when knots were present in 2015. Some beaches in 2015 had no knots (a beach with long-term aquaculture). Tide, intertidal location, and beach (name) determined the number of knots (and all shorebirds). Numbers decreased with distance from the mean high tide line. The average number of knots present in the intertidal mudflats two hours before or after low tide when knots were present (e.g. no censuses with zeros) was 2040 (=maximum flock size, in 2015
