摘要
应用超离心和柱层析结合聚丙烯酰胺凝胶电泳（ＰＡＧＥ）及电镜技术，研究中国鲎血蓝蛋白的结构特点．结果表明，经ＳｅｐｈａｄｅｘＧ－１００纯化的血蓝蛋白在ＰＡＧＥ电泳中出现４条带．纯化的血蓝蛋白再经ＤＥＡＥ－３２纤维素柱层析得到５个洗脱峰，每个峰在ＰＡＧＥ电泳下可分辨出４条带．在电镜下，血蓝蛋白分子出现环形、五角形、十字形和蝴蝶结形等构型并与其他解离的中间的构型同时存在．
By using ultracentrifugation,chromatography,polyacrylamide gel electron phoresis (PAGE) and electron microscopy,the structural characteristics of hemocyanin of T.tridentatus have been investigated.The results show that there are four bands in PAGE for hemocyanin purified by Sephadex G 100 chromatography. Purified hemocyanin can be separated by means of DEAE 32 cellulose chromatography into five peaks, marked as I～V.Four bands can be distinguished by PAGE for each peak.Under electron microscope,five typical view of T.tridentatus hemocyanin molecule can be observed according to different project angle.They look like ring,pentagon,cross,and bowtie in shape respectively.There are some of intermediate dissociating configuration of hemocyanin that can be seen in the same field.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第5期763-768,共6页
Journal of Xiamen University：Natural Science
基金
国家和福建省自然科学基金
