A two-month study was conducted to test the effects of macroalgae on the growth and survivorship of juvenile lined seahorse （Hippocarapus erectus） in aquaria. Twenty-day old seahorses were cultured in the tanks with...A two-month study was conducted to test the effects of macroalgae on the growth and survivorship of juvenile lined seahorse （Hippocarapus erectus） in aquaria. Twenty-day old seahorses were cultured in the tanks with green alga Chaetomorpha linum, red alga Gracilaria tikvahiae, green plastic artificial plant （stargrass）, and black plastic net, respectively. Increases in wet mass and standard length for the seahorses were significantly higher in the tanks with macroalgae than those without macroalgae. The seahorse survivorships in the two macroalgal treatments were also significantly higher than those of the two treatments without macroalgae. In the treatments with macroalgae, the DO and pH were higher than those without marcoalgae. During the experiment, the color of seahorses was changed to certain extent. In the green background （the treatments with C. linum and stargrass）, 24.1% 28.4% of black seahorses decreased over time; whereas in the black （plastic net） or brown （G. tikvahiae） background treatments, 14.1% 16.3% of yellow seahorses decreased over time, for matching that of the background. Furthermore, the survival rate of seahorse was correlation with DO and pH, and strong correlation with standard length, wet mass and CF. Polyculture with macroalgae, survival rates of seahorse were higher than without macroaglae.展开更多
为明确深圳某海马养殖基地线纹海马(Hippocampus erectus)突发性死亡病因,本研究从患病死亡线纹海马病灶组织分离到一株优势菌SZVA20190621,随后对分离菌的形态特征、生理生化特性、回归感染、16S r RNA基因进化及药物敏感性进行研究。...为明确深圳某海马养殖基地线纹海马(Hippocampus erectus)突发性死亡病因,本研究从患病死亡线纹海马病灶组织分离到一株优势菌SZVA20190621,随后对分离菌的形态特征、生理生化特性、回归感染、16S r RNA基因进化及药物敏感性进行研究。结果显示,该菌葡萄糖产气、甘露醇、覃糖、蔗糖、氧化酶活性和MR等生理生化鉴定为阳性;与NCBI上溶藻弧菌(Vibrio alginolyticus) KT986151具有98.16%的同源性;回归感染实验表明菌株SZVA20190621对线纹海马具有强致病性,LD_(50)为3.73×10^(5)CFU/mL。综上结果,鉴定该菌为致病性溶藻弧菌。药敏结果显示,该菌株对头孢唑林、头孢噻吩、哌拉西林、大观霉素、阿米卡星、克拉霉素、克林霉素及呋喃妥因耐药;对氨曲南、头孢西丁、头孢呋辛、头孢曲松、卡那霉素、红霉素中度敏感;对头孢他啶、头孢噻肟、头孢哌酮、头孢吡肟、链霉素、妥布霉素、庆大霉素、复方新诺明、氯霉素等17种抗生素高度敏感。本研究为线纹海马养殖中溶藻弧菌病的防控提供一定的参考依据。展开更多
基金The National Basic Research Program of China under contract No. 2006CB400608Ningbo Science and Technology Bureau under contract Nos 2005A610025 and 2006A610087K C Wong Magna Fund in Ningbo University
文摘A two-month study was conducted to test the effects of macroalgae on the growth and survivorship of juvenile lined seahorse （Hippocarapus erectus） in aquaria. Twenty-day old seahorses were cultured in the tanks with green alga Chaetomorpha linum, red alga Gracilaria tikvahiae, green plastic artificial plant （stargrass）, and black plastic net, respectively. Increases in wet mass and standard length for the seahorses were significantly higher in the tanks with macroalgae than those without macroalgae. The seahorse survivorships in the two macroalgal treatments were also significantly higher than those of the two treatments without macroalgae. In the treatments with macroalgae, the DO and pH were higher than those without marcoalgae. During the experiment, the color of seahorses was changed to certain extent. In the green background （the treatments with C. linum and stargrass）, 24.1% 28.4% of black seahorses decreased over time; whereas in the black （plastic net） or brown （G. tikvahiae） background treatments, 14.1% 16.3% of yellow seahorses decreased over time, for matching that of the background. Furthermore, the survival rate of seahorse was correlation with DO and pH, and strong correlation with standard length, wet mass and CF. Polyculture with macroalgae, survival rates of seahorse were higher than without macroaglae.
