Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal viruses.Enterovirus 71(EV71)is the primary pathogen that might cause severe symptoms and even deat...Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal viruses.Enterovirus 71(EV71)is the primary pathogen that might cause severe symptoms and even death in children with HFMD.This study aimed to investigate the intestinal detoxification time of HFMD children with EV71 infection and its related factors.Methods:Sixty-five HFMD children with EV71 infection were followed up.Their stool samples were collected once every 4 to 7 days.Viral nucleic acids were detected byfl uorescent polymerase chain reaction until the results became negative.The positive rates of viral nucleic acids were analyzed by the Kaplan-Meier method.The Log-rank test and Cox-Mantel test were used to analyze factors affecting the HFMD children with EV71 infection.Results:On the 2nd,4th,6th and 10th week,the positive rates of viral nucleic acids in stool samples of the 65 children were 94.6%,48.1%,17.2%and 0,respectively.Univariate analysis showed that the intestinal detoxification time of the children were related to gender,pre-admission disease course,severity of disease,and use of steroids or gamma globulin(P<0.05).Multivariate analysis showed that the severity of disease was an independent factor affecting the intestinal detoxification time(P<0.05),with a relative risk of 2.418.Conclusions:The longest intestinal detoxification time of HFMD children with EV71 infection was 10 weeks.The severity of disease was an important factor affecting the intestinal detoxification time of HFMD children with EV71 infection.Severe HFMD children with EV71 infection had a longer intestinal detoxification time.展开更多
In this manuscript the authors have studied the application value of White blood cell morphology combined with neutrophils alkaline phosphatase staining integral value in treatment of pediatric HFMD combined with infe...In this manuscript the authors have studied the application value of White blood cell morphology combined with neutrophils alkaline phosphatase staining integral value in treatment of pediatric HFMD combined with infection. They showed that comparing the NAP positive rate of HFMD co-infection with the NAP positive rate of other three groups respectively—reference group, HFMD group and anti-infection HFMD group—the t value is 25.7964, 28.2326, 28.3696 and the values of P are 0.0000;Comparing the NAP integral value of HFMD co-infection with the NAP integral value of other three groups respectively, which are reference group, HFMD group and anti-infection HFMD, the t value is 35.8687, 36.2664, 36.1374 and the values of P are 0.0000. They concluded that it is useful to apply WBC morphological examination combined with the positive rate and integral value of NAP staining to diagnose and treatment HFMD.展开更多
Capsid protein enterovirus 71 （EV71） is one of the major viruses that cause the severe encephalitis and thus result in a high mortality in children less than 5 years of age.In an effort to discover new potent inhibi...Capsid protein enterovirus 71 （EV71） is one of the major viruses that cause the severe encephalitis and thus result in a high mortality in children less than 5 years of age.In an effort to discover new potent inhibitors against EV71,a novel three-dimensional pharmacophore model was developed on 24 inhibitors with different molecular structures and bioactivities.The best hypothesis （Hypo1） has a high predictive power and consists of four features,namely,one hydrophobic point （HY） and three hydrogen-bond acceptors （HA）.Two key features of the best Hypo1,HY1 and HA3 match well with an important narrow hydrophobic canyon and with the surface of LYS274 in the target EV71 active site,respectively.The more versatile feature,HA1,is firstly found to be very influential on these compounds’ bioactivities,which may interact with the other side of the active site in the EV71 receptor.The application of the model is successful in predicting the activities of 30 known EV71 inhibitors with a correlation coefficient of 0.831.Furthermore,Hypo1 demonstrates a superior screening capability for retrieving inhibitors from the database with a high enrichment factor of 70.This study provides some important clues in search for more potent inhibitors against EV71 infection.展开更多
Objective:To study the effects of recombinant human interferon 2b atomization on serum cytokines and peripheral blood immune cells in children with HFMD.Methods: Children who were diagnosed with hand-foot-and-mouth di...Objective:To study the effects of recombinant human interferon 2b atomization on serum cytokines and peripheral blood immune cells in children with HFMD.Methods: Children who were diagnosed with hand-foot-and-mouth disease in Tongji Hospital? Tongji Medical College Huazhong University of Science & Technology between June 2014 and August 2017 were selected as the research subjects and randomly divided into the interferonα2b group who accepted human recombinant interferonα2b atomization combined with conventional symptomatic treatment and the control group who accepted conventional symptomatic treatment. The contents of cytokines in serum and the contents of immune cells in peripheral blood were determined before treatment as well as 3 days and 5 days after treatment. Results: 3 days and 5 days after treatment, IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of both groups of children were greatly lower than those before treatment whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those before treatment, and IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of interferonα2b group were significantly lower than those of control group whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those of control group.Conclusion: Recombinant human interferonα2b atomization treatment of HFMD can reduce the inflammatory response and improve the immune response.展开更多
Objective:To investigate the effects of lonicera granules + symptomatic treatment on serum indexes and immune function in children with hand-foot-and-mouth disease.Methods: A total of 120 children with HFMD who were t...Objective:To investigate the effects of lonicera granules + symptomatic treatment on serum indexes and immune function in children with hand-foot-and-mouth disease.Methods: A total of 120 children with HFMD who were treated between April 2016 and September 2017 were divided into the control group (n=60) and lonicera granules group (n=60) by random number table method. Control group received clinical symptomatic treatment for hand-foot-mouth disease, lonicera granules group received symptomatic treatment combined with lonicera granules therapy, and the treatment lasted for 7 d. The differences in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin were compared between the two groups before treatment and after 7 d of treatment.Results: Before treatment, there was no statistically significant difference in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin between the two groups. After 7 d of treatment, serum inflammatory cytokines IL-6, IL-10, IL-12 and TNF-α levels of lonicera granules group were lower than those of control group;serum myocardial enzyme spectrum indexesα-HBDH, LDH, AST and cTnⅠ levels were lower than those of control group;serum immunoglobulin IgA, IgG and IgM levels were higher than those of control group.Conclusion: Symptomatic treatment combined with lonicera granules therapy can further inhibit the systemic inflammatory response, protect the myocardial cells and enhance the immunity of children with HFMD.展开更多
基金supported by grants from the Health Science and Technology Program of Hangzhou(No.2011A039)the Medical Science and Technology Program of Zhejiang Province(No.2012KYB159).
文摘Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal viruses.Enterovirus 71(EV71)is the primary pathogen that might cause severe symptoms and even death in children with HFMD.This study aimed to investigate the intestinal detoxification time of HFMD children with EV71 infection and its related factors.Methods:Sixty-five HFMD children with EV71 infection were followed up.Their stool samples were collected once every 4 to 7 days.Viral nucleic acids were detected byfl uorescent polymerase chain reaction until the results became negative.The positive rates of viral nucleic acids were analyzed by the Kaplan-Meier method.The Log-rank test and Cox-Mantel test were used to analyze factors affecting the HFMD children with EV71 infection.Results:On the 2nd,4th,6th and 10th week,the positive rates of viral nucleic acids in stool samples of the 65 children were 94.6%,48.1%,17.2%and 0,respectively.Univariate analysis showed that the intestinal detoxification time of the children were related to gender,pre-admission disease course,severity of disease,and use of steroids or gamma globulin(P<0.05).Multivariate analysis showed that the severity of disease was an independent factor affecting the intestinal detoxification time(P<0.05),with a relative risk of 2.418.Conclusions:The longest intestinal detoxification time of HFMD children with EV71 infection was 10 weeks.The severity of disease was an important factor affecting the intestinal detoxification time of HFMD children with EV71 infection.Severe HFMD children with EV71 infection had a longer intestinal detoxification time.
文摘In this manuscript the authors have studied the application value of White blood cell morphology combined with neutrophils alkaline phosphatase staining integral value in treatment of pediatric HFMD combined with infection. They showed that comparing the NAP positive rate of HFMD co-infection with the NAP positive rate of other three groups respectively—reference group, HFMD group and anti-infection HFMD group—the t value is 25.7964, 28.2326, 28.3696 and the values of P are 0.0000;Comparing the NAP integral value of HFMD co-infection with the NAP integral value of other three groups respectively, which are reference group, HFMD group and anti-infection HFMD, the t value is 35.8687, 36.2664, 36.1374 and the values of P are 0.0000. They concluded that it is useful to apply WBC morphological examination combined with the positive rate and integral value of NAP staining to diagnose and treatment HFMD.
基金Sponsored by the National Natural Science Foundation of China (No. 30800719)
文摘Capsid protein enterovirus 71 （EV71） is one of the major viruses that cause the severe encephalitis and thus result in a high mortality in children less than 5 years of age.In an effort to discover new potent inhibitors against EV71,a novel three-dimensional pharmacophore model was developed on 24 inhibitors with different molecular structures and bioactivities.The best hypothesis （Hypo1） has a high predictive power and consists of four features,namely,one hydrophobic point （HY） and three hydrogen-bond acceptors （HA）.Two key features of the best Hypo1,HY1 and HA3 match well with an important narrow hydrophobic canyon and with the surface of LYS274 in the target EV71 active site,respectively.The more versatile feature,HA1,is firstly found to be very influential on these compounds’ bioactivities,which may interact with the other side of the active site in the EV71 receptor.The application of the model is successful in predicting the activities of 30 known EV71 inhibitors with a correlation coefficient of 0.831.Furthermore,Hypo1 demonstrates a superior screening capability for retrieving inhibitors from the database with a high enrichment factor of 70.This study provides some important clues in search for more potent inhibitors against EV71 infection.
基金National Program on Key Basic Research Project of China(973 Program)(No:2005CB5225007)Key Clinical Research Project of the Ministry of Health No.:2010-2012)National Science and Technology Support Program during the"11th Five-Year Plan”(No.:2006BAI05A07).
文摘Objective:To study the effects of recombinant human interferon 2b atomization on serum cytokines and peripheral blood immune cells in children with HFMD.Methods: Children who were diagnosed with hand-foot-and-mouth disease in Tongji Hospital? Tongji Medical College Huazhong University of Science & Technology between June 2014 and August 2017 were selected as the research subjects and randomly divided into the interferonα2b group who accepted human recombinant interferonα2b atomization combined with conventional symptomatic treatment and the control group who accepted conventional symptomatic treatment. The contents of cytokines in serum and the contents of immune cells in peripheral blood were determined before treatment as well as 3 days and 5 days after treatment. Results: 3 days and 5 days after treatment, IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of both groups of children were greatly lower than those before treatment whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those before treatment, and IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of interferonα2b group were significantly lower than those of control group whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those of control group.Conclusion: Recombinant human interferonα2b atomization treatment of HFMD can reduce the inflammatory response and improve the immune response.
文摘Objective:To investigate the effects of lonicera granules + symptomatic treatment on serum indexes and immune function in children with hand-foot-and-mouth disease.Methods: A total of 120 children with HFMD who were treated between April 2016 and September 2017 were divided into the control group (n=60) and lonicera granules group (n=60) by random number table method. Control group received clinical symptomatic treatment for hand-foot-mouth disease, lonicera granules group received symptomatic treatment combined with lonicera granules therapy, and the treatment lasted for 7 d. The differences in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin were compared between the two groups before treatment and after 7 d of treatment.Results: Before treatment, there was no statistically significant difference in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin between the two groups. After 7 d of treatment, serum inflammatory cytokines IL-6, IL-10, IL-12 and TNF-α levels of lonicera granules group were lower than those of control group;serum myocardial enzyme spectrum indexesα-HBDH, LDH, AST and cTnⅠ levels were lower than those of control group;serum immunoglobulin IgA, IgG and IgM levels were higher than those of control group.Conclusion: Symptomatic treatment combined with lonicera granules therapy can further inhibit the systemic inflammatory response, protect the myocardial cells and enhance the immunity of children with HFMD.
