本文介绍用不同浓度的氚水(HTO)一次腹腔注入怀孕12和15 d 的 C57 BL/6J 雌鼠,观察出生后3.5 d 第一代(F_1)仔鼠背部皮肤毛囊黑色素细胞异常率。结果表明,当15 d 龄胎鼠接受氚累积吸收剂量为0.11—0.67 Gy 时,毛囊异常率为1.0%—5.1%;12...本文介绍用不同浓度的氚水(HTO)一次腹腔注入怀孕12和15 d 的 C57 BL/6J 雌鼠,观察出生后3.5 d 第一代(F_1)仔鼠背部皮肤毛囊黑色素细胞异常率。结果表明,当15 d 龄胎鼠接受氚累积吸收剂量为0.11—0.67 Gy 时,毛囊异常率为1.0%—5.1%;12 d 龄胎鼠接受氚累积吸收剂量为0.12—0.46 Gy 时,毛囊异常率为3.4%—6.0%。说明随着氚累积吸收剂量的增加,毛囊异常率也增加;胚胎早期接受氚照射的 F_1代仔鼠皮肤毛囊异常率较高,12 d 龄胎鼠接受氚水照射引起的毛囊异常率比15 d 龄胎鼠的明显增高。展开更多
Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vect...Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide(H_2O_2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared. Results Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H_2O_2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Conclusion Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H_2O_2 through activating AKT signaling pathway.展开更多
文摘本文介绍用不同浓度的氚水(HTO)一次腹腔注入怀孕12和15 d 的 C57 BL/6J 雌鼠,观察出生后3.5 d 第一代(F_1)仔鼠背部皮肤毛囊黑色素细胞异常率。结果表明,当15 d 龄胎鼠接受氚累积吸收剂量为0.11—0.67 Gy 时,毛囊异常率为1.0%—5.1%;12 d 龄胎鼠接受氚累积吸收剂量为0.12—0.46 Gy 时,毛囊异常率为3.4%—6.0%。说明随着氚累积吸收剂量的增加,毛囊异常率也增加;胚胎早期接受氚照射的 F_1代仔鼠皮肤毛囊异常率较高,12 d 龄胎鼠接受氚水照射引起的毛囊异常率比15 d 龄胎鼠的明显增高。
基金supported by the Jilin Province Science and Technology Development Plan [20190304044YY]the Innovative special industry fund project in Jilin province [2018C049-2]+2 种基金the Joint construction project between Jilin province and provincial colleges [SXGJQY2017-12]the Open Research Project of the State Key Laboratory of Industrial Control Technology,Zhejiang University,China [ICT1800381]the China Natural National Science Foundation [81573067]
文摘Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide(H_2O_2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared. Results Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H_2O_2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Conclusion Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H_2O_2 through activating AKT signaling pathway.
