Onion（Allium cepa L.） is a very important vegetable crop widely cultivated throughout the world. In this study, we measured total anthocyanins and flavonols of onions of three colors. The fresh weight（FW） of total...Onion（Allium cepa L.） is a very important vegetable crop widely cultivated throughout the world. In this study, we measured total anthocyanins and flavonols of onions of three colors. The fresh weight（FW） of total anthocyanins were（29.99±1.19）,（9.64±1.30） and（0.75±0.40） mg 100 g^–1 fruit in red, yellow and white onions, respectively. Likewise, the FW of total flavonoids were（111.10±5.98）,（36.64±3.59）, and 0 mg 100 g^–1 in red, yellow and white, respectively. Four types of anthocyanins（delphinidin 3,5-diglycosides, cyanidin 3,5-diglycosides, cyanidin 3-glycosides and cyanidin 3-（6′′-malonyl）-glucopyranoside） and two kinds of flavonoids（quercetin and quercetin 3-glycosides） were identified in two varieties（red and yellow bulb） of onions by HPLC/DAD-ESI/MS. The total polyphenol contents were also measured by means of Folin-Ciocalteu method. Moreover, all the concentrations of anthocyanins, flavonoids and polyphenols showed significantly positive correlations with antioxidant activities measured by DPPH˙, ABTS˙＋ and FRAP assays. This study provided information on anthocyanin and flavonoids compositions that will be useful for onion breeding.展开更多
This work aims to elucidate the chemical constituents of Kai-Xin-San（KXS） and its metabolites in rat plasma.KXS extracts were separated on an Agilent HPLC SB-C 18 column,analyzed by ion-trap tandem mass spectrometry...This work aims to elucidate the chemical constituents of Kai-Xin-San（KXS） and its metabolites in rat plasma.KXS extracts were separated on an Agilent HPLC SB-C 18 column,analyzed by ion-trap tandem mass spectrometry and high-accuracy qTOF mass spectrometry in negative ion mode.A total of 39 compounds,including 11 ginsenosides,14 Polygala saponins,5 sucrose esters,8 oligosaccharide esters and 1 xanthone were characterized from KXS.Fifteen of them were confirmed by reference standards.No constituents were detected from Poria or Acori Tatarinowii Rhizoma.After oral administration of KXS（7 g/kg）,10 ginsenosides and 18 Polygala compounds were detected in rat plasma.This study indicates that ginseng saponins,Polygala saponins and saccharide esters could be the major effective components of KXS prescription.展开更多
Eight new 9,10-anthraquinones(1-8)including three acetonide derivatives of 3-hydroxy-2-hydroxymethyl-9,10-anthraquinones(68)were isolated from an ethanol extract of the roots of Knoxia valeriamoides.On the basis of ch...Eight new 9,10-anthraquinones(1-8)including three acetonide derivatives of 3-hydroxy-2-hydroxymethyl-9,10-anthraquinones(68)were isolated from an ethanol extract of the roots of Knoxia valeriamoides.On the basis of chemical transformation reactions of the co-occurring 14 and 15 combined with HPLC-DAD-ESI-MS analysis of the extracts,the previously and newly isolated 2-methoxymethy-and 2-ethoxymethyl-9,10-anthraquinones(4,5,and 9-13),as well as the 3-hydroxy-2-hydroxymethyl-9,10-anthraquinone acetonide derivatives(68),were shown to be solvolytic artifacts.In the in vitro assays,compound 4 was active to protect hepatocyte(WB-F344)damage.展开更多
基金financially supported by the Special Fund for Agro-scientific Research in the Public Interest,China(20090318)the Yangzhou Agricultural Science and Technology Research Projects,China(YZ2014170)
文摘Onion（Allium cepa L.） is a very important vegetable crop widely cultivated throughout the world. In this study, we measured total anthocyanins and flavonols of onions of three colors. The fresh weight（FW） of total anthocyanins were（29.99±1.19）,（9.64±1.30） and（0.75±0.40） mg 100 g^–1 fruit in red, yellow and white onions, respectively. Likewise, the FW of total flavonoids were（111.10±5.98）,（36.64±3.59）, and 0 mg 100 g^–1 in red, yellow and white, respectively. Four types of anthocyanins（delphinidin 3,5-diglycosides, cyanidin 3,5-diglycosides, cyanidin 3-glycosides and cyanidin 3-（6′′-malonyl）-glucopyranoside） and two kinds of flavonoids（quercetin and quercetin 3-glycosides） were identified in two varieties（red and yellow bulb） of onions by HPLC/DAD-ESI/MS. The total polyphenol contents were also measured by means of Folin-Ciocalteu method. Moreover, all the concentrations of anthocyanins, flavonoids and polyphenols showed significantly positive correlations with antioxidant activities measured by DPPH˙, ABTS˙＋ and FRAP assays. This study provided information on anthocyanin and flavonoids compositions that will be useful for onion breeding.
基金National Science & Technology Mega Project forPrimary Drug Innovation from Ministry of Science and Technologyof China (Grant No. 2009ZX09502-006)
文摘This work aims to elucidate the chemical constituents of Kai-Xin-San（KXS） and its metabolites in rat plasma.KXS extracts were separated on an Agilent HPLC SB-C 18 column,analyzed by ion-trap tandem mass spectrometry and high-accuracy qTOF mass spectrometry in negative ion mode.A total of 39 compounds,including 11 ginsenosides,14 Polygala saponins,5 sucrose esters,8 oligosaccharide esters and 1 xanthone were characterized from KXS.Fifteen of them were confirmed by reference standards.No constituents were detected from Poria or Acori Tatarinowii Rhizoma.After oral administration of KXS（7 g/kg）,10 ginsenosides and 18 Polygala compounds were detected in rat plasma.This study indicates that ginseng saponins,Polygala saponins and saccharide esters could be the major effective components of KXS prescription.
基金Financial support from the National Natural Sciences Foundation of China(NNSFC,grant Nos.30825044 and 20932007)the National Science and Technology Project of China(No.2009ZX09311-004)is acknowledged.
文摘Eight new 9,10-anthraquinones(1-8)including three acetonide derivatives of 3-hydroxy-2-hydroxymethyl-9,10-anthraquinones(68)were isolated from an ethanol extract of the roots of Knoxia valeriamoides.On the basis of chemical transformation reactions of the co-occurring 14 and 15 combined with HPLC-DAD-ESI-MS analysis of the extracts,the previously and newly isolated 2-methoxymethy-and 2-ethoxymethyl-9,10-anthraquinones(4,5,and 9-13),as well as the 3-hydroxy-2-hydroxymethyl-9,10-anthraquinone acetonide derivatives(68),were shown to be solvolytic artifacts.In the in vitro assays,compound 4 was active to protect hepatocyte(WB-F344)damage.
