Objective:To investigate the association between the polymorphism of HLA-A,HLA-B genes and pre-eclampsia. Methods:HLA-A,HLA-B genotyping was performed by polymerase chain reaction sequence-specific primer (PCR-SSP) in...Objective:To investigate the association between the polymorphism of HLA-A,HLA-B genes and pre-eclampsia. Methods:HLA-A,HLA-B genotyping was performed by polymerase chain reaction sequence-specific primer (PCR-SSP) in 119 preeclampsia patients,117 normal pregnant women and their neonates. Results:The study showed that 16 HLA-A and 39 HLA-B alleles were obtained in pre-eclamptic patients and normal pregnant women. 15 HLA-A and 37 HLA-B alleles were obtained in their neonates. No significant difference was found in maternal or neonatal HLA-A,HLA-B alleles between pre-eclampsia group and control group (Pc>0.05). The frequencies of HLA-A11,HLA-A24,HLA-B13,HLA-B14,HLA-B15,HLA-B52 maternal/fetus genetic assoications were significantly diffe-rent between pre-eclampsia group and control group (P<0.05). Conclusion:Some HLA-A,HLA-B maternal/fetus special bindings may be associated with the susceptibility or protective of pre-eclampsia.展开更多
目的鉴定1例人类白细胞抗原（human leukocyte antigen，HLA）新等位基因。方法应用双链及单链测序法进行基于序列的分型（sequence based typing，SBT），通过群体调查了解该等位基因的群体分布频率。结果该等位基因HLA—A＊33：44，与...目的鉴定1例人类白细胞抗原（human leukocyte antigen，HLA）新等位基因。方法应用双链及单链测序法进行基于序列的分型（sequence based typing，SBT），通过群体调查了解该等位基因的群体分布频率。结果该等位基因HLA—A＊33：44，与A＊33：03：01第4外显子相差1个核苷酸，使第866位碱基由G变为A，第265个密码子由GGT变为GAT，相应氨基酸由甘氨酸变为天门冬氨酸。与IMGT／HLA数据库中的HLA—A等位基因的序列进行对比，该突变为新的单核苷酸多态性位点。结论该新HLA等位基因在中国汉族人群中的分布频率小于0．0003，并被世界卫生组织HLA因子命名委员会命名为HLA—A＊33：44（序列注册号为HQ873871）。研究新等位基因的序列可为HLA基因相关研究和应用提供信息。展开更多
基金Supported by National Natural Science Foundation of China (30872316)~~
文摘Objective:To investigate the association between the polymorphism of HLA-A,HLA-B genes and pre-eclampsia. Methods:HLA-A,HLA-B genotyping was performed by polymerase chain reaction sequence-specific primer (PCR-SSP) in 119 preeclampsia patients,117 normal pregnant women and their neonates. Results:The study showed that 16 HLA-A and 39 HLA-B alleles were obtained in pre-eclamptic patients and normal pregnant women. 15 HLA-A and 37 HLA-B alleles were obtained in their neonates. No significant difference was found in maternal or neonatal HLA-A,HLA-B alleles between pre-eclampsia group and control group (Pc>0.05). The frequencies of HLA-A11,HLA-A24,HLA-B13,HLA-B14,HLA-B15,HLA-B52 maternal/fetus genetic assoications were significantly diffe-rent between pre-eclampsia group and control group (P<0.05). Conclusion:Some HLA-A,HLA-B maternal/fetus special bindings may be associated with the susceptibility or protective of pre-eclampsia.
文摘目的鉴定1例人类白细胞抗原（human leukocyte antigen，HLA）新等位基因。方法应用双链及单链测序法进行基于序列的分型（sequence based typing，SBT），通过群体调查了解该等位基因的群体分布频率。结果该等位基因HLA—A＊33：44，与A＊33：03：01第4外显子相差1个核苷酸，使第866位碱基由G变为A，第265个密码子由GGT变为GAT，相应氨基酸由甘氨酸变为天门冬氨酸。与IMGT／HLA数据库中的HLA—A等位基因的序列进行对比，该突变为新的单核苷酸多态性位点。结论该新HLA等位基因在中国汉族人群中的分布频率小于0．0003，并被世界卫生组织HLA因子命名委员会命名为HLA—A＊33：44（序列注册号为HQ873871）。研究新等位基因的序列可为HLA基因相关研究和应用提供信息。
