目的:通过追踪观察小鼠细胞牙骨质发育过程中的组织形态和细胞活性改变,探讨源于上皮根鞘断裂的上皮性细胞是否参与细胞牙骨质的发育。方法:选取出生后15、19、25 d BALB/c小鼠的下颌第一磨牙根尖1/3区细胞牙骨质,细胞角蛋白14(CK 14)...目的:通过追踪观察小鼠细胞牙骨质发育过程中的组织形态和细胞活性改变,探讨源于上皮根鞘断裂的上皮性细胞是否参与细胞牙骨质的发育。方法:选取出生后15、19、25 d BALB/c小鼠的下颌第一磨牙根尖1/3区细胞牙骨质,细胞角蛋白14(CK 14)标记追踪上皮根鞘断裂后的上皮性细胞;TUNEL(Td T-mediated-d UTP nick end labeling,TUNEL)法检测细胞牙骨质在发育过程中的细胞凋亡情况;透射电镜观察细胞牙骨质发育的超微结构。结果:细胞凋亡检测结果显示,细胞牙骨质形成过程中可见大量被埋入或正被埋入细胞呈现凋亡阳性表达;免疫组化染色结果显示,其中部分细胞呈CK14阳性表达;透射电镜观察结果显示,细胞牙骨质形成过程中上皮性细胞被其外侧成牙骨质细胞分泌的基质包围。结论:来源上皮根鞘断裂的上皮性细胞可能作为牙骨质细胞参与细胞牙骨质的形成。展开更多
Occlusion is commenced by contact of a tooth with an opposing tooth and is the mechanical force working against the periodontal ligament (PDL). Our recent study indicated that occlusion regulated tooth root elongation...Occlusion is commenced by contact of a tooth with an opposing tooth and is the mechanical force working against the periodontal ligament (PDL). Our recent study indicated that occlusion regulated tooth root elongation occurs during root development in rat molars. Using a non-occlusal model established to directly examine the effects of the absence of occlusion in developing first molars of upper jaw, histological analysis was performed to count the number of HERS cells, with Microarray used to analyse gene expression profiles. HERS cell numbers in normal molars decreased significantly more than those in experimental molars. In microarray data, a total of 59 genes showed significant differences (fold change > 2.0). Expressions of 55 genes in the experimental molars, which included PLAP-1/asporin and periostin, were significantly decreased than those in normal molars. These data indicate that occlusion during root development leads to a decrease in the number of HERS cells, and that the aforementioned genes may play an essential role in normal root formation.展开更多
Anisotropic metallic Nanoparticles （NPs） have unique optical properties, such as Surface Enhanced Raman Scattering （SERS） spearoscopy. In this paper, star-shaped and sphere gold NPs were prepared by seed-mediated ...Anisotropic metallic Nanoparticles （NPs） have unique optical properties, such as Surface Enhanced Raman Scattering （SERS） spearoscopy. In this paper, star-shaped and sphere gold NPs were prepared by seed-mediated growth and Frence methods respectively. The reaction process arid the effect of reagent in seed-mediated growth of gold naaostar particles were systematically described. After fabricaring NPs the authors test their Raman enhancement using Ch-ystal Violet （CV） moleolles apart. The experimental results indicated that star-shaped Au NPs had strtmger Raman enhancement spectrum than that of sphere Au NPs.展开更多
联合应用靶向HER1（ErbB1，EGFR）的抗肿瘤药吉非替尼与传统化疗药紫杉醇，观察HERs高表达胃癌细胞的生长抑制作用及对HERs的影响。吉非替尼（Gefilinib，lressa）是苯胺喹哟啉类HER1酪氨酸激酶抑制剂（TKIs）；紫杉醇（Paclitaxel，Ta...联合应用靶向HER1（ErbB1，EGFR）的抗肿瘤药吉非替尼与传统化疗药紫杉醇，观察HERs高表达胃癌细胞的生长抑制作用及对HERs的影响。吉非替尼（Gefilinib，lressa）是苯胺喹哟啉类HER1酪氨酸激酶抑制剂（TKIs）；紫杉醇（Paclitaxel，Taxol）是抗微管药，二者通过不同作用机制引发肿瘤细胞凋亡。胃癌在我国是高发生率、高死亡率的肿瘤，目前针对胃癌的靶向治疗或联合靶向药物与传统化疗药物的研究较少，而胃癌中33％-74％存在HER1的高表达。联合应用HER1靶向药物吉非替尼与化疗药紫杉醇，考察其合用时的疗效，相互作用（协同，相加等），及可能的作用机制是本研究的主要目的。研究中先采用MTT法考察单独给予两药对不同HERs表达水平胃癌细胞的抑制作用。再采用MTT法检测两药不同比例联合应用对HERs高表达胃癌细胞的抑制作用，在此基础上通过计算CI值（Combination Index Value），考察两药合用于HERs高表达胃癌细胞株的相互作用关系（协同或相加）。展开更多
文摘目的:通过追踪观察小鼠细胞牙骨质发育过程中的组织形态和细胞活性改变,探讨源于上皮根鞘断裂的上皮性细胞是否参与细胞牙骨质的发育。方法:选取出生后15、19、25 d BALB/c小鼠的下颌第一磨牙根尖1/3区细胞牙骨质,细胞角蛋白14(CK 14)标记追踪上皮根鞘断裂后的上皮性细胞;TUNEL(Td T-mediated-d UTP nick end labeling,TUNEL)法检测细胞牙骨质在发育过程中的细胞凋亡情况;透射电镜观察细胞牙骨质发育的超微结构。结果:细胞凋亡检测结果显示,细胞牙骨质形成过程中可见大量被埋入或正被埋入细胞呈现凋亡阳性表达;免疫组化染色结果显示,其中部分细胞呈CK14阳性表达;透射电镜观察结果显示,细胞牙骨质形成过程中上皮性细胞被其外侧成牙骨质细胞分泌的基质包围。结论:来源上皮根鞘断裂的上皮性细胞可能作为牙骨质细胞参与细胞牙骨质的形成。
文摘Occlusion is commenced by contact of a tooth with an opposing tooth and is the mechanical force working against the periodontal ligament (PDL). Our recent study indicated that occlusion regulated tooth root elongation occurs during root development in rat molars. Using a non-occlusal model established to directly examine the effects of the absence of occlusion in developing first molars of upper jaw, histological analysis was performed to count the number of HERS cells, with Microarray used to analyse gene expression profiles. HERS cell numbers in normal molars decreased significantly more than those in experimental molars. In microarray data, a total of 59 genes showed significant differences (fold change > 2.0). Expressions of 55 genes in the experimental molars, which included PLAP-1/asporin and periostin, were significantly decreased than those in normal molars. These data indicate that occlusion during root development leads to a decrease in the number of HERS cells, and that the aforementioned genes may play an essential role in normal root formation.
文摘Anisotropic metallic Nanoparticles （NPs） have unique optical properties, such as Surface Enhanced Raman Scattering （SERS） spearoscopy. In this paper, star-shaped and sphere gold NPs were prepared by seed-mediated growth and Frence methods respectively. The reaction process arid the effect of reagent in seed-mediated growth of gold naaostar particles were systematically described. After fabricaring NPs the authors test their Raman enhancement using Ch-ystal Violet （CV） moleolles apart. The experimental results indicated that star-shaped Au NPs had strtmger Raman enhancement spectrum than that of sphere Au NPs.
文摘联合应用靶向HER1（ErbB1，EGFR）的抗肿瘤药吉非替尼与传统化疗药紫杉醇，观察HERs高表达胃癌细胞的生长抑制作用及对HERs的影响。吉非替尼（Gefilinib，lressa）是苯胺喹哟啉类HER1酪氨酸激酶抑制剂（TKIs）；紫杉醇（Paclitaxel，Taxol）是抗微管药，二者通过不同作用机制引发肿瘤细胞凋亡。胃癌在我国是高发生率、高死亡率的肿瘤，目前针对胃癌的靶向治疗或联合靶向药物与传统化疗药物的研究较少，而胃癌中33％-74％存在HER1的高表达。联合应用HER1靶向药物吉非替尼与化疗药紫杉醇，考察其合用时的疗效，相互作用（协同，相加等），及可能的作用机制是本研究的主要目的。研究中先采用MTT法考察单独给予两药对不同HERs表达水平胃癌细胞的抑制作用。再采用MTT法检测两药不同比例联合应用对HERs高表达胃癌细胞的抑制作用，在此基础上通过计算CI值（Combination Index Value），考察两药合用于HERs高表达胃癌细胞株的相互作用关系（协同或相加）。
