Objective:To study the chemical constituents of stembark of Garcinia malaccenm（G.malaccenm） together with apoptotic.antimicrobial and antioxidant activities.Methods:Purification and structure elucidation were carrie...Objective:To study the chemical constituents of stembark of Garcinia malaccenm（G.malaccenm） together with apoptotic.antimicrobial and antioxidant activities.Methods:Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively.MTT and trypan blue exclusion methods were performed to study the cytotoxic activity.Antibacterial activity was conducted by dise diffusion and microdilulion methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging.Results:The phylochemical study led lo the isolation ofα,β-mangostin and cycloarl-24-en-3β-ol.α-Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC<sub>50</sub> of 0.33μM.β- andα-mangostin showed activity against K562 cells with IC<sub>50</sub> of 0.40μM and 0.48μM,respectively,α-Mangostin was active against Gram-positive bacteria, Staphylococcus aureus（S.aureus） and Bacilus anthracis（B.anthmcis） with inhibition zone and MIC value of（19 mm;0.02S mg/mL） and（20 mm;0.013 mg/mL）,respectively.In antioxidant assay,α-mangostin exhibited activity as an inhibitor of lipid peroxidation.Conclusions:G.malaccenm presenceα- andβ-mangostin and cycloart-24-en-3β-ol.β-Mangostin was found very active against H.SC-3 cells and KS62.The results suggest that mangoslins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis.In addition,α-andβ-mangostin was found inhibit the growth of Cram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation.展开更多
A new xanthone, 1,2-dihydro-3,6,8-trihydroxy-1,1-bis (3-methylbut -2-enyl)-5-(1, 1-dimethylprop-2-enyl)xanthen-2,9-dione (1), has been isolated from the aerial part of Hypericum erectum.
Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japon...Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum. Their structures were determined by spectroscopic and chemical methods.展开更多
Four new polycyclic polyprenylated acylphloroglucinol type metabolites,hypercohones D–G(1–4),along with four known analogues(5–8),were isolated from the aerial parts of Hypericum cohaerens.The structures of these i...Four new polycyclic polyprenylated acylphloroglucinol type metabolites,hypercohones D–G(1–4),along with four known analogues(5–8),were isolated from the aerial parts of Hypericum cohaerens.The structures of these isolates were elucidated by extensive spectroscopic methods.The inhibitory activities of these isolates against five human cancer cell lines in vitro were also tested.展开更多
基金partially supported by the Intermtional Islamic University Malaysia(HUM)through EDWB1002-350
文摘Objective:To study the chemical constituents of stembark of Garcinia malaccenm（G.malaccenm） together with apoptotic.antimicrobial and antioxidant activities.Methods:Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively.MTT and trypan blue exclusion methods were performed to study the cytotoxic activity.Antibacterial activity was conducted by dise diffusion and microdilulion methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging.Results:The phylochemical study led lo the isolation ofα,β-mangostin and cycloarl-24-en-3β-ol.α-Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC<sub>50</sub> of 0.33μM.β- andα-mangostin showed activity against K562 cells with IC<sub>50</sub> of 0.40μM and 0.48μM,respectively,α-Mangostin was active against Gram-positive bacteria, Staphylococcus aureus（S.aureus） and Bacilus anthracis（B.anthmcis） with inhibition zone and MIC value of（19 mm;0.02S mg/mL） and（20 mm;0.013 mg/mL）,respectively.In antioxidant assay,α-mangostin exhibited activity as an inhibitor of lipid peroxidation.Conclusions:G.malaccenm presenceα- andβ-mangostin and cycloart-24-en-3β-ol.β-Mangostin was found very active against H.SC-3 cells and KS62.The results suggest that mangoslins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis.In addition,α-andβ-mangostin was found inhibit the growth of Cram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation.
文摘A new xanthone, 1,2-dihydro-3,6,8-trihydroxy-1,1-bis (3-methylbut -2-enyl)-5-(1, 1-dimethylprop-2-enyl)xanthen-2,9-dione (1), has been isolated from the aerial part of Hypericum erectum.
文摘Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum. Their structures were determined by spectroscopic and chemical methods.
基金supported by the National Natural Science Foundation (Nos 20862005 and 20562004)the Program for New Century Excellent Talents in University (NCET-08-0656)~~
基金the foundations from NSFC(20972167)the Young Academic Leader Raising Foundation of Yunnan Province(No.2009CI073)the foundation from CAS to Dr G.Xu.
文摘Four new polycyclic polyprenylated acylphloroglucinol type metabolites,hypercohones D–G(1–4),along with four known analogues(5–8),were isolated from the aerial parts of Hypericum cohaerens.The structures of these isolates were elucidated by extensive spectroscopic methods.The inhibitory activities of these isolates against five human cancer cell lines in vitro were also tested.
