Objective: To observe the efficacy and safety of Guihuang Formula(GHF) in treating patients with type Ⅲ prostatitis and Chinese medicine syndrome of dampness-heat and blood stasis. Methods: Sixty-six type Ⅲ prostati...Objective: To observe the efficacy and safety of Guihuang Formula(GHF) in treating patients with type Ⅲ prostatitis and Chinese medicine syndrome of dampness-heat and blood stasis. Methods: Sixty-six type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome were randomly divided into the treatment group(GHF) and the control group(tamsulosin) using a random number table, with 33 cases each group. The treatment group received GHF twice a day, and the control group received tamsulosin 0.2 mg once daily before bedtime. Patients in both groups received treatment for 6 weeks and was followed up for 2 weeks. The outcomes included the National Institute of Health Chronic Prostatitis Symptom Index(NIH-CPSI) score, Chinese Medicine Symptoms Score(CMSS), expressed prostatic secretions(EPS) and adverse events(AEs). Results: After treatment, the NIH-CPSI total score and domain scores of pain discomfort, urination and quality of life decreased significantly from the baseline in both groups(P<0.05). The CMSS score decreased in both groups(P<0.05). The white blood cell(WBC) count decreased and lecithin body count increased in both groups(P<0.05). GHF showed a more obvious advantage in reducing the pain discomfort and quality of life domain scores of NIH-CPSI, reducing the CMSS score, increasing the improvement rate of the WBC and lecithin body counts, compared with the control group(P<0.05). There were no significant differences in decreasing urination domain score of NIH-CPSI between two groups(P>0.05). In addition, no serious AEs were observed. Conclusion: GHF is effective in treating type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome without serious AEs.(Registration No. ChiCTR1900026966)展开更多
[Objectives]To explore the mechanism of Angelica sinensis-Phellodendri Chinensis Cortex in the treatment of chronic prostatitis(CP)based on the method of network pharmacology.[Methods]The active components and action ...[Objectives]To explore the mechanism of Angelica sinensis-Phellodendri Chinensis Cortex in the treatment of chronic prostatitis(CP)based on the method of network pharmacology.[Methods]The active components and action targets of Angelica sinensis-Phellodendri Chinensis Cortex were screened by(TCMSP),a systematic pharmacological analysis platform of traditional Chinese medicine,combined with literature search.The target was corrected by Uniprot database,and the disease CP target was screened by GeneCards and OMIM database.The common targets of drugs and diseases were screened by R language software,and the visual network map of drugs-active components-targets-diseases was constructed by Cytoscape 3.5.1 software.The common target protein-protein interaction(PPI)network was constructed by using STRING platform.The R language software was used to annotate and analyze the gene function and pathway of the core target through geneontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).[Results]46 active components of Angelica sinensis-Phellodendri Chinensis Cortex were screened,and 212 related targets were predicted,of which 159 were related to disease.These targets were mainly involved in biological processes such as heterologous biological stimulation,oxidation and anti-oxidation,and were mainly concentrated in PI3K-Akt,mitogen-activated protein kinase(MAPK),hypoxia inducible factor-1(HIF-1)and other related signaling pathways.[Conclusions]The multi-component,multi-target and multi-pathway action characteristics of Angelica sinensis-Phellodendri Chinensis Cortex were confirmed by network pharmacology,and the possible mechanism of Angelica sinensis-Phellodendri Chinensis Cortex in the treatment of CP was predicted,which provided a theoretical basis for further experiments to verify its action mechanism.展开更多
目的:基于网络药理学方法探讨当归-黄柏治疗慢性前列腺炎(chronic prostatitis,CP)的作用机制。方法:应用中药系统药理学分析平台(TCMSP)并结合文献检索,筛选当归-黄柏中的活性成分与作用靶点,利用Uniprot数据库对靶点进行校正,应用Gene...目的:基于网络药理学方法探讨当归-黄柏治疗慢性前列腺炎(chronic prostatitis,CP)的作用机制。方法:应用中药系统药理学分析平台(TCMSP)并结合文献检索,筛选当归-黄柏中的活性成分与作用靶点,利用Uniprot数据库对靶点进行校正,应用GeneCards、OMIM数据库筛选疾病CP靶点,应用R语言软件筛选药物与疾病共同靶点,应用Cytoscape3.5.1软件构建药物-活性成分-靶点-疾病可视化网络图,应用STRING平台构建共同靶点蛋白互作(protein-protein interaction,PPI)网络,利用R语言软件通过基因功能注释(gene ontology,GO)和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)对核心靶标进行基因功能和通路的注释分析。结果:筛选出46个当归-黄柏的活性成分,预测相关靶点212个,其中与疾病有关的靶点159个,这些靶点主要涉及异源生物刺激反应、氧化与抗氧化反应等生物学过程,并主要富集在PI3K-Akt、丝裂原活化蛋白激酶(mitogenactivated protein kinase,MAPK)、低氧诱导因子-1(hypoxia inducible factor-1,HIF-1)等相关信号通路上。结论:通过网络药理学证实了当归-黄柏多成分、多靶点、多途径的作用特点,预测当归-黄柏治疗CP的可能作用机制,为进一步实验验证其作用机制提供了理论依据。展开更多
基金Supported by the National Natural Science Foundation of China (No.82104880)Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences (No.CI2021A02208)the Nursery Project of Xiyuan Hospital of China Academy of Chinese Medical Sciences (No.2019XYMP-23)。
文摘Objective: To observe the efficacy and safety of Guihuang Formula(GHF) in treating patients with type Ⅲ prostatitis and Chinese medicine syndrome of dampness-heat and blood stasis. Methods: Sixty-six type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome were randomly divided into the treatment group(GHF) and the control group(tamsulosin) using a random number table, with 33 cases each group. The treatment group received GHF twice a day, and the control group received tamsulosin 0.2 mg once daily before bedtime. Patients in both groups received treatment for 6 weeks and was followed up for 2 weeks. The outcomes included the National Institute of Health Chronic Prostatitis Symptom Index(NIH-CPSI) score, Chinese Medicine Symptoms Score(CMSS), expressed prostatic secretions(EPS) and adverse events(AEs). Results: After treatment, the NIH-CPSI total score and domain scores of pain discomfort, urination and quality of life decreased significantly from the baseline in both groups(P<0.05). The CMSS score decreased in both groups(P<0.05). The white blood cell(WBC) count decreased and lecithin body count increased in both groups(P<0.05). GHF showed a more obvious advantage in reducing the pain discomfort and quality of life domain scores of NIH-CPSI, reducing the CMSS score, increasing the improvement rate of the WBC and lecithin body counts, compared with the control group(P<0.05). There were no significant differences in decreasing urination domain score of NIH-CPSI between two groups(P>0.05). In addition, no serious AEs were observed. Conclusion: GHF is effective in treating type Ⅲ prostatitis patients with dampness-heat and blood stasis syndrome without serious AEs.(Registration No. ChiCTR1900026966)
基金Nursery Project of Xiyuan Hospital of China Academy of Chinese Medical Sciences(2019XYMP-23)Clinical Study of Guihuang Prescription in the Treatment of Chronic Prostatitis/Chronic Pelvic Pain Syndrome Based on"Internal Elimination Method for Ulcer"+1 种基金National Natural Science Foundation Cultivation Project of Xiyuan Hospital of China Academy of Chinese Medical Sciences(XY20-13)Study on the Mechanism of Guihuang Prescription in the Treatment of Prostatitis III Based on PI3K/Akt/NF-κB Signaling Pathway.
文摘[Objectives]To explore the mechanism of Angelica sinensis-Phellodendri Chinensis Cortex in the treatment of chronic prostatitis(CP)based on the method of network pharmacology.[Methods]The active components and action targets of Angelica sinensis-Phellodendri Chinensis Cortex were screened by(TCMSP),a systematic pharmacological analysis platform of traditional Chinese medicine,combined with literature search.The target was corrected by Uniprot database,and the disease CP target was screened by GeneCards and OMIM database.The common targets of drugs and diseases were screened by R language software,and the visual network map of drugs-active components-targets-diseases was constructed by Cytoscape 3.5.1 software.The common target protein-protein interaction(PPI)network was constructed by using STRING platform.The R language software was used to annotate and analyze the gene function and pathway of the core target through geneontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).[Results]46 active components of Angelica sinensis-Phellodendri Chinensis Cortex were screened,and 212 related targets were predicted,of which 159 were related to disease.These targets were mainly involved in biological processes such as heterologous biological stimulation,oxidation and anti-oxidation,and were mainly concentrated in PI3K-Akt,mitogen-activated protein kinase(MAPK),hypoxia inducible factor-1(HIF-1)and other related signaling pathways.[Conclusions]The multi-component,multi-target and multi-pathway action characteristics of Angelica sinensis-Phellodendri Chinensis Cortex were confirmed by network pharmacology,and the possible mechanism of Angelica sinensis-Phellodendri Chinensis Cortex in the treatment of CP was predicted,which provided a theoretical basis for further experiments to verify its action mechanism.
文摘目的:基于网络药理学方法探讨当归-黄柏治疗慢性前列腺炎(chronic prostatitis,CP)的作用机制。方法:应用中药系统药理学分析平台(TCMSP)并结合文献检索,筛选当归-黄柏中的活性成分与作用靶点,利用Uniprot数据库对靶点进行校正,应用GeneCards、OMIM数据库筛选疾病CP靶点,应用R语言软件筛选药物与疾病共同靶点,应用Cytoscape3.5.1软件构建药物-活性成分-靶点-疾病可视化网络图,应用STRING平台构建共同靶点蛋白互作(protein-protein interaction,PPI)网络,利用R语言软件通过基因功能注释(gene ontology,GO)和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)对核心靶标进行基因功能和通路的注释分析。结果:筛选出46个当归-黄柏的活性成分,预测相关靶点212个,其中与疾病有关的靶点159个,这些靶点主要涉及异源生物刺激反应、氧化与抗氧化反应等生物学过程,并主要富集在PI3K-Akt、丝裂原活化蛋白激酶(mitogenactivated protein kinase,MAPK)、低氧诱导因子-1(hypoxia inducible factor-1,HIF-1)等相关信号通路上。结论:通过网络药理学证实了当归-黄柏多成分、多靶点、多途径的作用特点,预测当归-黄柏治疗CP的可能作用机制,为进一步实验验证其作用机制提供了理论依据。
