目的探讨弓形虫可溶性抗原(STAg)和γ干扰素(IFN-γ)联合鼻内免疫对小鼠的保护作用及IFN-γ作为佐剂鼻内免疫抗弓形虫感染的最佳剂量.方法将5～6周龄BALB/c小鼠70只随机分为5组,每组14只,分别用20μg STAg、20μg STAg+250 U IFN-γ、20...目的探讨弓形虫可溶性抗原(STAg)和γ干扰素(IFN-γ)联合鼻内免疫对小鼠的保护作用及IFN-γ作为佐剂鼻内免疫抗弓形虫感染的最佳剂量.方法将5～6周龄BALB/c小鼠70只随机分为5组,每组14只,分别用20μg STAg、20μg STAg+250 U IFN-γ、20 μg STAg+500 UIFN-γ、20μg STAg+1 000 U IFN-γ和20μg STAg+2 000 U IFN-γ鼻内免疫小鼠2次,间隔14 d,末次免疫后第10天,用RH株弓形虫速殖子4×104个/只灌胃攻击,逐日观察小鼠健康存活情况.攻击后第29天处死全部小鼠,分离计数脑、肝组织速殖子;计数肠系膜淋巴结(MLN)和脾T淋巴细胞.结果随着佐剂IFN-γ剂量的增加,小鼠存活率有升高趋势,1 000 U IFN-γ剂量组小鼠存活率最高达93%;肝、脑速殖子数呈下降的趋势,其中STAg+1 000 U IFN-γ、STAg+2 000 UIFN-γ组显著低于STAg组;MLN和脾T淋巴细胞发生了增殖性应答,其中STAg+1 000 UIFN-γ、STAg+2 000 U IFN-γ组MLN细胞显著高于STAg组.结论STAg+1 000 U IFN-γ、STAg+2 000 U IFN-γ鼻内免疫明显优于其它小剂量佐剂及单独抗原免疫,能有效诱导黏膜免疫应答.展开更多
Objective： To study the influence of Yanyankang Powder （眼炎康散） on Th1/Th2 in rats with experimental autoimmune uveitis （EAU）. Methods： The EAU models were induced in Lewis rats by immunization with interphoto...Objective： To study the influence of Yanyankang Powder （眼炎康散） on Th1/Th2 in rats with experimental autoimmune uveitis （EAU）. Methods： The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein （IRBP） 1177-1191 in complete Freund＇s adjuvant. The rats were randomly divided into 3 groups： a model control group, a Yanyankang group, and a prednisone group, 9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/（kg·day） by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/（kg·d） by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon （INF-γ） and interleukin-10 （IL-10） in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction （PCR） technology was used for measuring Thl and Th2 related cytokine mRNA expressions. Results： Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γ and IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30, 155.87±4.46 μg/L in the Yanyankang group and 270.93 ± 7.76, 265.32 ± 11.88 μg/L in the prednisone group. Both had significant differences compared with the control group （941.53± 8.59, 20.67± 4.65 μg/L; P=0.01）. The PCR results showed the same tendency. Conclusion： Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells.展开更多
文摘目的探讨弓形虫可溶性抗原(STAg)和γ干扰素(IFN-γ)联合鼻内免疫对小鼠的保护作用及IFN-γ作为佐剂鼻内免疫抗弓形虫感染的最佳剂量.方法将5～6周龄BALB/c小鼠70只随机分为5组,每组14只,分别用20μg STAg、20μg STAg+250 U IFN-γ、20 μg STAg+500 UIFN-γ、20μg STAg+1 000 U IFN-γ和20μg STAg+2 000 U IFN-γ鼻内免疫小鼠2次,间隔14 d,末次免疫后第10天,用RH株弓形虫速殖子4×104个/只灌胃攻击,逐日观察小鼠健康存活情况.攻击后第29天处死全部小鼠,分离计数脑、肝组织速殖子;计数肠系膜淋巴结(MLN)和脾T淋巴细胞.结果随着佐剂IFN-γ剂量的增加,小鼠存活率有升高趋势,1 000 U IFN-γ剂量组小鼠存活率最高达93%;肝、脑速殖子数呈下降的趋势,其中STAg+1 000 U IFN-γ、STAg+2 000 UIFN-γ组显著低于STAg组;MLN和脾T淋巴细胞发生了增殖性应答,其中STAg+1 000 UIFN-γ、STAg+2 000 U IFN-γ组MLN细胞显著高于STAg组.结论STAg+1 000 U IFN-γ、STAg+2 000 U IFN-γ鼻内免疫明显优于其它小剂量佐剂及单独抗原免疫,能有效诱导黏膜免疫应答.
基金Supported by the National Natural Science Foundation of China(No.81072961,81100658 and 81373826)the National Science Foundation for Post-doctoral Scientists of China(No.2012M510068)
文摘Objective： To study the influence of Yanyankang Powder （眼炎康散） on Th1/Th2 in rats with experimental autoimmune uveitis （EAU）. Methods： The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein （IRBP） 1177-1191 in complete Freund＇s adjuvant. The rats were randomly divided into 3 groups： a model control group, a Yanyankang group, and a prednisone group, 9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/（kg·day） by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/（kg·d） by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon （INF-γ） and interleukin-10 （IL-10） in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction （PCR） technology was used for measuring Thl and Th2 related cytokine mRNA expressions. Results： Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γ and IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30, 155.87±4.46 μg/L in the Yanyankang group and 270.93 ± 7.76, 265.32 ± 11.88 μg/L in the prednisone group. Both had significant differences compared with the control group （941.53± 8.59, 20.67± 4.65 μg/L; P=0.01）. The PCR results showed the same tendency. Conclusion： Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells.
