摘要
目的研究T辅助细胞不同功能亚群在慢性荨麻疹发病机制中的作用。方法采用Ficoil-hypaque密度梯度离心法,分离30例慢性荨麻疹患者外周血单一核细胞(PBMC),检测PBMC体外培养在植物血清素(PHA)诱导后Th1类细胞因子,γ干扰素(Interferon-gamma,IFN-γ)和Th2类细胞因子,白细胞介素4(Interleukin-4,IL-4)的变化,并设立20例正常对照。结果慢性荨麻疹患者组的PBMC在PHA诱导下产生IL-4水平明显高于正常对照组(P<0.01),而IFN-γ的诱导水平则低于正常对照组(P<0.01)。结论慢性荨麻疹患者外周血存在着Th细胞亚群分化失衡,这可能为慢性荨麻疹发病的重要机制之一。
Objective To investigate the clinical signifieanees of levels of interleukin - 4（IL - 4） and interferon - gamma（IFN - γ） in serum and peripheral blood mononuelear cells（PBMCs） from patientts with chronic urtearia, Methods The effects of IL - 4 and IFN- γ in PBMCs and serum of patients with chronic urtearia were determined by using Fieoil - hypaque density gradient centrifuge. Results The level of IL - 4 produced by PBMC induced by PHA in the observation group was higher than that of the controls, showing significant difference （ P 〈 0.05）. The levels of IFN - γin serum in the observation group was lower than that of the control group, also showing a significant difference （ P 〈 0.05）. Conclusion The differentiation of Th cell subgroup in peripheral blood of patients with chronic urticaria is abnormal and this might be one of an important mechanism in the pathogenesis of chronic urticaria.
出处
《中国热带医学》
CAS
2007年第7期1113-1114,共2页
China Tropical Medicine
