BACKGROUND Diarrhea is a major infectious cause of childhood morbidity and mortality worldwide.In clinical trials,Lactobacillus rhamnosus GG ATCC 53013(LGG)has been used to treat diarrhea.However,recent randomized con...BACKGROUND Diarrhea is a major infectious cause of childhood morbidity and mortality worldwide.In clinical trials,Lactobacillus rhamnosus GG ATCC 53013(LGG)has been used to treat diarrhea.However,recent randomized controlled trials(RCTs)found no evidence of a beneficial effect of LGG treatment.AIM To evaluate the efficacy of LGG in treating acute diarrhea in children.METHODS The EMBASE,MEDLINE,PubMed,Web of Science databases,and the Cochrane Central Register of Controlled Trials were searched up to April 2019 for metaanalyses and RCTs.The Cochrane Review Manager was used to analyze the relevant data.RESULTS Nineteen RCTs met the inclusion criteria and showed that compared with the control group,LGG administration notably reduced the diarrhea duration[mean difference(MD)-24.02 h,95%confidence interval(CI)(-36.58,-11.45)].More effective results were detected at a high dose≥1010 CFU per day[MD-22.56 h,95%CI(-36.41,-8.72)]vs a lower dose.A similar reduction was found in Asian and European patients[MD-24.42 h,95%CI(-47.01,-1.82);MD-32.02 h,95%CI(-49.26,-14.79),respectively].A reduced duration of diarrhea was confirmed in LGG participants with diarrhea for less than 3 d at enrollment[MD-15.83 h,95%CI(-20.68,-10.98)].High-dose LGG effectively reduced the duration of rotavirus-induced diarrhea[MD-31.05 h,95%CI(-50.31,-11.80)]and the stool number per day[MD-1.08,95%CI(-1.87,-0.28)].CONCLUSION High-dose LGG therapy reduces the duration of diarrhea and the stool number per day.Intervention at the early stage is recommended.Future trials are expected to verify the effectiveness of LGG treatment.展开更多
AIM: To investigate the effects of a low fermentable, oligosaccharides, disaccharides, monosaccharides and polyols diet (LFD) and the probiotic Lactobacillus rhamnosus GG (LGG) in irritable bowel syndrome (IBS).
AIM To investigate the adhesion and anti-inflammatory effects of Lactobacil us rhamnosus GG (LGG) in the colonic mucosa of healthy and ulcerative colitis (UC) patients, both in vivo and ex vivo in an organ culture mod...AIM To investigate the adhesion and anti-inflammatory effects of Lactobacil us rhamnosus GG (LGG) in the colonic mucosa of healthy and ulcerative colitis (UC) patients, both in vivo and ex vivo in an organ culture model.METHODS For the ex vivo experiment, a total of 98 patients (68 UC patients and 30 normal subjects) were included. Endoscopic biopsies were collected and incubated with and without LGG or LGG-conditioned media to evaluate the mucosal adhesion and anti-inflammatory effects [reduction of tumor necrosis factor alpha (TNFα) and interleukin (IL)-17 expression] of the bacteria, and extraction of DNA and RNA for quantification by real-time (RT)-PCR occurred after the incubation. A dose-response study was performed by incubating biopsies at "regular", double and 5 times higher doses of LGG. For the in vivo experiment, a total of 42 patients (20 UC patients and 22 normal controls) were included. Biopsies were taken from the colons of normal subjects who consumed a commercial formulation of LGG for 7 d prior to the colonoscopy,and the adhesion of the bacteria to the colonic mucosa was evaluated by RT-PCR and compared with that of control biopsies from patients who did not consume the formulation. LGG adhesion and TNFα and IL-17 expression were compared between UC patients who consumed a regular or double dose of LGG supplementation prior to colonoscopy.RESULTS In the ex vivo experiment, LGG showed consistent adhesion to the distal and proximal colon in normal subjects and UC patients, with a trend towards higher concentrations in the distal colon, and in UC patients, adhesion was similar in biopsies with active and quiescent inflammation. In addition, bioptic samples from UC patients incubated with LGG conditioned media (CM) showed reduced expression of TNFα and IL-17 compared with the corresponding expression in controls (P < 0.05). Incubation with a double dose of LGG increased mucosal adhesion and the anti-inflammatory effects (P < 0.05). In the in vivo experiment, LGG was detectable only in the colon 展开更多
Background: Deoxynivalenol(DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG(LGG) is a probiotic that not only has anti-i...Background: Deoxynivalenol(DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG(LGG) is a probiotic that not only has anti-inflammatory effects, but also shows protective effect on the intestinal barrier. However, it is still unknown whether LGG exerts beneficial effects against DON-induced intestinal damage in piglets. In this work, a total of 36 weaned piglets were randomized to one of four treatment groups for 21 d. The treatment groups were CON(basal diet);LGG(basal diet supplemented with 1.77 × 10^(11)CFU/kg LGG);DON(DON-contaminated diet) and LGG + DON(DON-contaminated diet supplemented with 1.77 × 10^(11)CFU/kg LGG).Result: Supplementation of LGG can enhance growth performance of piglets exposed to DON by improving intestinal barrier function. LGG has a mitigating effect on intestinal inflammation induced by DON exposure, largely through repression of the TLR4/NF-κB signaling pathway. Furthermore, supplementation of LGG increased the relative abundances of beneficial bacteria(e.g., Collinsella, Lactobacillus, Ruminococcus_torques_group and Anaerofustis), and decreased the relative abundances of harmful bacteria(e.g., Parabacteroides and Ruminiclostridium_6), and also promoted the production of SCFAs.Conclusions: LGG ameliorates DON-induced intestinal damage, which may provide theoretical support for the application of LGG to alleviate the adverse effects induced by DON exposure.展开更多
Classification is always the key point in the field of remote sensing. Fuzzy c-Means is a traditional clustering algorithm that has been widely used in fuzzy clustering. However, this algorithm usually has some weakne...Classification is always the key point in the field of remote sensing. Fuzzy c-Means is a traditional clustering algorithm that has been widely used in fuzzy clustering. However, this algorithm usually has some weaknesses, such as the problems of falling into a local minimum, and it needs much time to accomplish the classification for a large number of data. In order to overcome these shortcomings and increase the classifi-cation accuracy, Gustafson-Kessel (GK) and Gath-Geva (GG) algorithms are proposed to improve the tradi-tional FCM algorithm which adopts Euclidean distance norm in this paper. The experimental result shows that these two methods are able to detect clusters of varying shapes, sizes and densities which FCM cannot do. Moreover, they can improve the classification accuracy of remote sensing images.展开更多
To overcome the mutual coupling (MC) of multiple antennas in millimeter wave (mmWave) communication systems, a novel beam design method with low complexity is proposed in this paper. Firstly, an equivalent channel mod...To overcome the mutual coupling (MC) of multiple antennas in millimeter wave (mmWave) communication systems, a novel beam design method with low complexity is proposed in this paper. Firstly, an equivalent channel model incorporating the effect of MC is analyzed and established, and then an optimal precoding / combiner vector for beamforming is derived. On this basis, by using greedy geometric (GG) algorithm, a novel hybrid beam design method is proposed. Finally, the performance of proposed method is analyzed and compared with other traditional ones. The simulation results show that the proposed method has better suppression of the MC effect as well as lower complexity.展开更多
AIM:To study the effects of live and dead Lactobacillus rhamnosus GG(GG) on rotavirus infection in a neonatal rat model.METHODS:At the age of 2 d,suckling Lewis rat pups were supplemented with either live or dead GG a...AIM:To study the effects of live and dead Lactobacillus rhamnosus GG(GG) on rotavirus infection in a neonatal rat model.METHODS:At the age of 2 d,suckling Lewis rat pups were supplemented with either live or dead GG and the treatment was continued daily throughout the experi-ment.At the age of 5 and 6 d the pups received oral rotavirus(RV) SA-11 strain.The pups were sacrificed at the age of 7 or 8 d by decapitation.The gastrointestinal tract was removed and macroscopic observations were done.The consistency of feces in the colon was classified using a four-tier system.RV was detected from the plasma,small intestine,colon and feces by real-time quantitative polymerase chain reaction(PCR).RESULTS:In this neonatal rat model,RV induced a mild-to-moderate diarrhea in all except one pup of the RV-inoculated rats.RV moderately reduced body weight development from day 6 onwards.On day 7,after 2 d of RV infection,live and dead GG groups gained significantly more weight than the RV group without probiotics [36%(P = 0.001) and 28%(P = 0.031),respectively].In addition,when compared with the RV control group,both live and dead GG reduced the weight ratio of colon/animal body weight to the same level as in the healthy control group,with reductions of 22%(P = 0.002) and 28%(P < 0.001),respectively.Diarrhea increased moderately in both GG groups.However,the diarrhea incidence and severity in the GG groups were not statistically significantly different as compared with the RV control group.Moreover,observed diarrhea did not provoke weight loss or death.The RV control group had the largest amount of RV PCR-positive samples among the RV-infected groups,and the live GG group had the smallest amount.Rats receiving live GG had significantly less RV in the colon(P = 0.027) when compared with the RV control group.Live GG was also more effective over dead GG in reducing the quantity of RV from plasma(P = 0.047).CONCLUSION:Both live and dead GG have beneficial effects in RV infection.GG may increase RV clearance from the body and reduce c展开更多
Objective: To determine if Lactobacillus GG (LGG) is an effective adjunct to lactulose for treating constipation in children. Study design: Eighty- four chi ldren (2- 16 years of age) with constipation (< 3 spontan...Objective: To determine if Lactobacillus GG (LGG) is an effective adjunct to lactulose for treating constipation in children. Study design: Eighty- four chi ldren (2- 16 years of age) with constipation (< 3 spontaneous bowel movements [ BMs] per week for at least 12 weeks) were enrolled in a double- blind, randomiz ed placebo- controlled trial in which they received 1 mL/kg/day of 70% lactul ose plus 109 colony- forming units (CFU) of LGG (experimental group, n = 43) or a placebo (control group, n = 41) orally twice daily for 12 weeks. The primary outcome measure was treatment success, and analyses were performed on an intenti on- to- treat basis. Results: Treatment success, defined as ≥ 3 spontaneous B Ms per week with no fecal soiling, was similar in the control and experimental g roups at 12 weeks (28/41 [68% ] vs 31/43 [72% ], respectively; P =. 7) and at 24 weeks (27/41 [65% ] vs 27/42 [64% ], respectively; P = 1.0]. Groups also di d not differ in their mean number of spontaneous BMs per week or episodes of fec al soiling per week at 4, 8, and 12 weeks. Adverse events and overall tolerance did not differ between groups. Conclusion: LGG, as dosed in this study, was not an effective adjunct to lactulose in treating constipation in children.展开更多
目的采用GC法同时测定不同干燥方式不同部位草珊瑚挥发油中a-蒎稀、蒎稀、(-)-β-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯8种成分的含量.方法色谱柱:HP-5毛细管色谱柱(30m x 0.32mm x 0.25μm);检测器:FID;进样口温度:...目的采用GC法同时测定不同干燥方式不同部位草珊瑚挥发油中a-蒎稀、蒎稀、(-)-β-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯8种成分的含量.方法色谱柱:HP-5毛细管色谱柱(30m x 0.32mm x 0.25μm);检测器:FID;进样口温度:250T;检测器温度:270t;柱温:5℃为起始温度,保持2min,以•min^-1的速度升至130℃,保持5min;载气:氮气;流速:1 mL•min^-1;进样量:lμL;分流比:30:1结果a-疲烯、蒎稀、(-)-R-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、P-榄香烯和β-石竹烯色谱峰分离度良好;进样浓度分别在8.75-1750.00,5.62~1123.60,8.66-1732.80,4.37~873.60 1.96~391.20,4.98-995.60,8.54-1707.60,3.61~721.40μg·mL^-1范围线性良好;平均加样回收率(n=6)分别为98.7%、97.2%、98.2%,98.3%、97.7%、97.5%、9&2%、97.8%,RSD分别为0.62%、1.21%,1.24%、1.30%、1.32%、1.27%、1.57%、0.77%。12批草珊瑚挥发油中a-疲烯、蒎稀、(-)-β-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯的含量(n=3)测定结果分别为0~225.50、0~167.06、0~250.34、0~55.66、0~77.91、0~201.98、2.35~294.49、0~14.82.结论该方法准确可靠,适用于草珊瑚挥发油中8个活性成分的含量测定.展开更多
Electrospinning was used to produce ultrafine fibers and fibrous mats from aqueous solutions containing two edible polysaccharides: pectin (PEC) and pullulan (PUL). The process excluded the use of a synthetic carrier ...Electrospinning was used to produce ultrafine fibers and fibrous mats from aqueous solutions containing two edible polysaccharides: pectin (PEC) and pullulan (PUL). The process excluded the use of a synthetic carrier polymer or non-aqueous solvents thus maintaining the food-grade status of the components. The inclusion of PUL reduced the surface tension and electric conductivity of pectin solution, and promoted molecular entanglement between PEC and PUL as confirmed by rheological analysis. The spinnability of either polysaccharide was promoted by inclusion of the other. Cross-linking PEC networks were obtained by soaking the fibrous mats in successive Ca<sup>2+</sup> solution. The fibrous mats can be used to carry bioactive compounds as demonstrated using probiotic bacteria Lactobacillus rhamnosus GG (LGG) as a model bioactive compound. The research is significant for the development of food products with unique textures and functionalities.展开更多
基金Supported by the National Natural Science Foundation of China,No.81330011
文摘BACKGROUND Diarrhea is a major infectious cause of childhood morbidity and mortality worldwide.In clinical trials,Lactobacillus rhamnosus GG ATCC 53013(LGG)has been used to treat diarrhea.However,recent randomized controlled trials(RCTs)found no evidence of a beneficial effect of LGG treatment.AIM To evaluate the efficacy of LGG in treating acute diarrhea in children.METHODS The EMBASE,MEDLINE,PubMed,Web of Science databases,and the Cochrane Central Register of Controlled Trials were searched up to April 2019 for metaanalyses and RCTs.The Cochrane Review Manager was used to analyze the relevant data.RESULTS Nineteen RCTs met the inclusion criteria and showed that compared with the control group,LGG administration notably reduced the diarrhea duration[mean difference(MD)-24.02 h,95%confidence interval(CI)(-36.58,-11.45)].More effective results were detected at a high dose≥1010 CFU per day[MD-22.56 h,95%CI(-36.41,-8.72)]vs a lower dose.A similar reduction was found in Asian and European patients[MD-24.42 h,95%CI(-47.01,-1.82);MD-32.02 h,95%CI(-49.26,-14.79),respectively].A reduced duration of diarrhea was confirmed in LGG participants with diarrhea for less than 3 d at enrollment[MD-15.83 h,95%CI(-20.68,-10.98)].High-dose LGG effectively reduced the duration of rotavirus-induced diarrhea[MD-31.05 h,95%CI(-50.31,-11.80)]and the stool number per day[MD-1.08,95%CI(-1.87,-0.28)].CONCLUSION High-dose LGG therapy reduces the duration of diarrhea and the stool number per day.Intervention at the early stage is recommended.Future trials are expected to verify the effectiveness of LGG treatment.
文摘AIM: To investigate the effects of a low fermentable, oligosaccharides, disaccharides, monosaccharides and polyols diet (LFD) and the probiotic Lactobacillus rhamnosus GG (LGG) in irritable bowel syndrome (IBS).
文摘AIM To investigate the adhesion and anti-inflammatory effects of Lactobacil us rhamnosus GG (LGG) in the colonic mucosa of healthy and ulcerative colitis (UC) patients, both in vivo and ex vivo in an organ culture model.METHODS For the ex vivo experiment, a total of 98 patients (68 UC patients and 30 normal subjects) were included. Endoscopic biopsies were collected and incubated with and without LGG or LGG-conditioned media to evaluate the mucosal adhesion and anti-inflammatory effects [reduction of tumor necrosis factor alpha (TNFα) and interleukin (IL)-17 expression] of the bacteria, and extraction of DNA and RNA for quantification by real-time (RT)-PCR occurred after the incubation. A dose-response study was performed by incubating biopsies at "regular", double and 5 times higher doses of LGG. For the in vivo experiment, a total of 42 patients (20 UC patients and 22 normal controls) were included. Biopsies were taken from the colons of normal subjects who consumed a commercial formulation of LGG for 7 d prior to the colonoscopy,and the adhesion of the bacteria to the colonic mucosa was evaluated by RT-PCR and compared with that of control biopsies from patients who did not consume the formulation. LGG adhesion and TNFα and IL-17 expression were compared between UC patients who consumed a regular or double dose of LGG supplementation prior to colonoscopy.RESULTS In the ex vivo experiment, LGG showed consistent adhesion to the distal and proximal colon in normal subjects and UC patients, with a trend towards higher concentrations in the distal colon, and in UC patients, adhesion was similar in biopsies with active and quiescent inflammation. In addition, bioptic samples from UC patients incubated with LGG conditioned media (CM) showed reduced expression of TNFα and IL-17 compared with the corresponding expression in controls (P < 0.05). Incubation with a double dose of LGG increased mucosal adhesion and the anti-inflammatory effects (P < 0.05). In the in vivo experiment, LGG was detectable only in the colon
基金supported by the Natural Science Foundation of Heilongjiang Province(TD2019C001)the National Natural Science Foundation of China(U21A20251)the State Key Program of National Natural Science Foundation of China(32030101).
文摘Background: Deoxynivalenol(DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG(LGG) is a probiotic that not only has anti-inflammatory effects, but also shows protective effect on the intestinal barrier. However, it is still unknown whether LGG exerts beneficial effects against DON-induced intestinal damage in piglets. In this work, a total of 36 weaned piglets were randomized to one of four treatment groups for 21 d. The treatment groups were CON(basal diet);LGG(basal diet supplemented with 1.77 × 10^(11)CFU/kg LGG);DON(DON-contaminated diet) and LGG + DON(DON-contaminated diet supplemented with 1.77 × 10^(11)CFU/kg LGG).Result: Supplementation of LGG can enhance growth performance of piglets exposed to DON by improving intestinal barrier function. LGG has a mitigating effect on intestinal inflammation induced by DON exposure, largely through repression of the TLR4/NF-κB signaling pathway. Furthermore, supplementation of LGG increased the relative abundances of beneficial bacteria(e.g., Collinsella, Lactobacillus, Ruminococcus_torques_group and Anaerofustis), and decreased the relative abundances of harmful bacteria(e.g., Parabacteroides and Ruminiclostridium_6), and also promoted the production of SCFAs.Conclusions: LGG ameliorates DON-induced intestinal damage, which may provide theoretical support for the application of LGG to alleviate the adverse effects induced by DON exposure.
基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
文摘Classification is always the key point in the field of remote sensing. Fuzzy c-Means is a traditional clustering algorithm that has been widely used in fuzzy clustering. However, this algorithm usually has some weaknesses, such as the problems of falling into a local minimum, and it needs much time to accomplish the classification for a large number of data. In order to overcome these shortcomings and increase the classifi-cation accuracy, Gustafson-Kessel (GK) and Gath-Geva (GG) algorithms are proposed to improve the tradi-tional FCM algorithm which adopts Euclidean distance norm in this paper. The experimental result shows that these two methods are able to detect clusters of varying shapes, sizes and densities which FCM cannot do. Moreover, they can improve the classification accuracy of remote sensing images.
基金supported by Aeronautical Science Foundation of China (2017ZC52021)the Major Program of National Natural Science Foundation of China (61827801)
文摘To overcome the mutual coupling (MC) of multiple antennas in millimeter wave (mmWave) communication systems, a novel beam design method with low complexity is proposed in this paper. Firstly, an equivalent channel model incorporating the effect of MC is analyzed and established, and then an optimal precoding / combiner vector for beamforming is derived. On this basis, by using greedy geometric (GG) algorithm, a novel hybrid beam design method is proposed. Finally, the performance of proposed method is analyzed and compared with other traditional ones. The simulation results show that the proposed method has better suppression of the MC effect as well as lower complexity.
基金Supported by Valio Ltd and the Finnish Funding Agency of Technology and InnovationThe Foundation for Nutrition Research has financially contributed to Liisa Lehtoranta’s doctoral studies
文摘AIM:To study the effects of live and dead Lactobacillus rhamnosus GG(GG) on rotavirus infection in a neonatal rat model.METHODS:At the age of 2 d,suckling Lewis rat pups were supplemented with either live or dead GG and the treatment was continued daily throughout the experi-ment.At the age of 5 and 6 d the pups received oral rotavirus(RV) SA-11 strain.The pups were sacrificed at the age of 7 or 8 d by decapitation.The gastrointestinal tract was removed and macroscopic observations were done.The consistency of feces in the colon was classified using a four-tier system.RV was detected from the plasma,small intestine,colon and feces by real-time quantitative polymerase chain reaction(PCR).RESULTS:In this neonatal rat model,RV induced a mild-to-moderate diarrhea in all except one pup of the RV-inoculated rats.RV moderately reduced body weight development from day 6 onwards.On day 7,after 2 d of RV infection,live and dead GG groups gained significantly more weight than the RV group without probiotics [36%(P = 0.001) and 28%(P = 0.031),respectively].In addition,when compared with the RV control group,both live and dead GG reduced the weight ratio of colon/animal body weight to the same level as in the healthy control group,with reductions of 22%(P = 0.002) and 28%(P < 0.001),respectively.Diarrhea increased moderately in both GG groups.However,the diarrhea incidence and severity in the GG groups were not statistically significantly different as compared with the RV control group.Moreover,observed diarrhea did not provoke weight loss or death.The RV control group had the largest amount of RV PCR-positive samples among the RV-infected groups,and the live GG group had the smallest amount.Rats receiving live GG had significantly less RV in the colon(P = 0.027) when compared with the RV control group.Live GG was also more effective over dead GG in reducing the quantity of RV from plasma(P = 0.047).CONCLUSION:Both live and dead GG have beneficial effects in RV infection.GG may increase RV clearance from the body and reduce c
文摘Objective: To determine if Lactobacillus GG (LGG) is an effective adjunct to lactulose for treating constipation in children. Study design: Eighty- four chi ldren (2- 16 years of age) with constipation (< 3 spontaneous bowel movements [ BMs] per week for at least 12 weeks) were enrolled in a double- blind, randomiz ed placebo- controlled trial in which they received 1 mL/kg/day of 70% lactul ose plus 109 colony- forming units (CFU) of LGG (experimental group, n = 43) or a placebo (control group, n = 41) orally twice daily for 12 weeks. The primary outcome measure was treatment success, and analyses were performed on an intenti on- to- treat basis. Results: Treatment success, defined as ≥ 3 spontaneous B Ms per week with no fecal soiling, was similar in the control and experimental g roups at 12 weeks (28/41 [68% ] vs 31/43 [72% ], respectively; P =. 7) and at 24 weeks (27/41 [65% ] vs 27/42 [64% ], respectively; P = 1.0]. Groups also di d not differ in their mean number of spontaneous BMs per week or episodes of fec al soiling per week at 4, 8, and 12 weeks. Adverse events and overall tolerance did not differ between groups. Conclusion: LGG, as dosed in this study, was not an effective adjunct to lactulose in treating constipation in children.
文摘目的采用GC法同时测定不同干燥方式不同部位草珊瑚挥发油中a-蒎稀、蒎稀、(-)-β-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯8种成分的含量.方法色谱柱:HP-5毛细管色谱柱(30m x 0.32mm x 0.25μm);检测器:FID;进样口温度:250T;检测器温度:270t;柱温:5℃为起始温度,保持2min,以•min^-1的速度升至130℃,保持5min;载气:氮气;流速:1 mL•min^-1;进样量:lμL;分流比:30:1结果a-疲烯、蒎稀、(-)-R-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、P-榄香烯和β-石竹烯色谱峰分离度良好;进样浓度分别在8.75-1750.00,5.62~1123.60,8.66-1732.80,4.37~873.60 1.96~391.20,4.98-995.60,8.54-1707.60,3.61~721.40μg·mL^-1范围线性良好;平均加样回收率(n=6)分别为98.7%、97.2%、98.2%,98.3%、97.7%、97.5%、9&2%、97.8%,RSD分别为0.62%、1.21%,1.24%、1.30%、1.32%、1.27%、1.57%、0.77%。12批草珊瑚挥发油中a-疲烯、蒎稀、(-)-β-蒎稀、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯的含量(n=3)测定结果分别为0~225.50、0~167.06、0~250.34、0~55.66、0~77.91、0~201.98、2.35~294.49、0~14.82.结论该方法准确可靠,适用于草珊瑚挥发油中8个活性成分的含量测定.
文摘Electrospinning was used to produce ultrafine fibers and fibrous mats from aqueous solutions containing two edible polysaccharides: pectin (PEC) and pullulan (PUL). The process excluded the use of a synthetic carrier polymer or non-aqueous solvents thus maintaining the food-grade status of the components. The inclusion of PUL reduced the surface tension and electric conductivity of pectin solution, and promoted molecular entanglement between PEC and PUL as confirmed by rheological analysis. The spinnability of either polysaccharide was promoted by inclusion of the other. Cross-linking PEC networks were obtained by soaking the fibrous mats in successive Ca<sup>2+</sup> solution. The fibrous mats can be used to carry bioactive compounds as demonstrated using probiotic bacteria Lactobacillus rhamnosus GG (LGG) as a model bioactive compound. The research is significant for the development of food products with unique textures and functionalities.
