Summary:Ranolazine,a late sodium current inhibitor,has been demonstrated to be effective on heart failure.18B-glycyrrhetinic acid(18β-GA)has the similar inhibitory effect on late sodium currents.However,its effect on...Summary:Ranolazine,a late sodium current inhibitor,has been demonstrated to be effective on heart failure.18B-glycyrrhetinic acid(18β-GA)has the similar inhibitory effect on late sodium currents.However,its effect on diastolic function is still unknown.This study aimed to determine whether 18β-GA can improve the diastolic function and to explore the underlying mechanisms.Eighty male Sprague Dawley(SD)rats of Langendorff model were randomly divided into the following groups:group A,normal cardiac perfusion group;group B,ischemia-reperfusion group;group C,ischemia-reperfusion with anemoniasulcata toxinⅡ(ATX-Ⅱ);group D,ranolazine group;and group E,18β-GA group with four different concentrations.Furthermore,a pressure-overloaded rat model induced by trans-aortic constriction(TAC)was established.Echocardiography and hemodynamics were used to evaluate diastolic function at 14th day after TAC.Changes of free intracellular calcium(Ca27)concentration was indirectly detected by laser scanning confocal microscope to confirm the inhibition of late sodium currents.With the intervention of ATX-Ⅱon ischemia reperfusion injury group,5 umol/L ranolazine,and 5,10,20,40μmol/L 18β-GA could improve ATX-I-induced cardiac diastolic dysfunction.630 mg/kg glycyrrhizin tablets could improve cardiac diastolic function in the pressure-overloaded rats.18B-GA and ranolazine had similar effects on reducing the free calcium in cardiomyocytes.The study demonstrates that 18B-GA and glycyrrhizin could improve diastolic dysfunction induced by ischemia-reperfusion injury in Langendorff-perfused rat hearts and pressure-overloaded rats.The mechanism may be attributed to the inhibition of enhanced late sodium currents.展开更多
目的探索接受血液透析的终末期肾病(end-stage renal disease,ESRD)患者液体状态和肺功能之间的关系。方法2019年4月~10月,纳入在辽阳市中心医院血液净化中心接受血液透析的ESRD患者54例。在透析前和透析后,测量患者的呼吸功能,同时采...目的探索接受血液透析的终末期肾病(end-stage renal disease,ESRD)患者液体状态和肺功能之间的关系。方法2019年4月~10月,纳入在辽阳市中心医院血液净化中心接受血液透析的ESRD患者54例。在透析前和透析后,测量患者的呼吸功能,同时采用电阻抗分析方法(bioimpedance analysis,BIA)测量患者的水负荷(overhydration,OH)和细胞外液(extracellular water,ECW),并计算2者的百分比(OH/ECW%)。OH/ECW%>7%视为液体超载。结果血液透析后用力肺活量(forced vital capacity,FVC)、FVC占预计量的百分比(forced vital capacity as a percentage of predicted normal value,FVC%)和第1秒的用力呼气容积(forced expiratory volume in one second,FEV_(1))水平显著升高,而OH/ECW%降低(t值分别为2.213、2.389、2.327、15.117,P值分别为0.030、0.019、0.022、<0.001)。透析前共有15例(27.8%)患者发生了液体超载。液体超载组的FVC、FVC%、FEV_(1)、FEV_(1)占预计量的百分比(FEV_(1)as a percentage of predicted normal value,FEV_(1)%)、用力呼出气量为25%~75%肺活量时的平均流量(forced expiratory flow between 25%and 75%of the FVC,FEF_(25-75))、呼出肺活量25%~75%时的用力呼气流量占预计量百分比(forced expiratory flow between 25%and 75%of the FVC as a percentage of predicted normal values,FEF_(25-75)%)、呼气峰值流速(peak expiratory flow rate,PEFR)、呼气峰值流速占预计量的百分比(peak expiratory flow rate as a percentage of predicted normal value,PEFR%)均低于无液体超载组(t值分别为4.740、5.131、4.902、6.226、4.263、3.668、2.904、3.694,P值分别为<0.001、<0.001、<0.001、<0.001、<0.001、<0.001、0.005、<0.001)。透析前OH/ECW%与FVC、FVC%、FEV_(1)、FEV_(1)%、FEF25–75、FEF25–75%、PEFR、PEFR%呈负相关(r值分别为–0.411、–0.530、–0.395、–0.491、–0.307、–0.272、–0.302、–0.425,P值分别为0.002、<0.001、0.003、<0.001、0.024、0.047、0.027、0.001)。多元线性回归分析模型显示,透析展开更多
Objective： To investigate the effect of Yiqi Huoxue Recipe （YHR, 益气活血方） on the cardiac function and ultrastructure during the regression of myocardial hypertrophy induced by pressure overload in rats. Methods...Objective： To investigate the effect of Yiqi Huoxue Recipe （YHR, 益气活血方） on the cardiac function and ultrastructure during the regression of myocardial hypertrophy induced by pressure overload in rats. Methods： The model of myocardial hypertrophy was established by abdominal aortic banding. Eighty male Wistar rats were divided into six groups, the normal control group Ⅰ （n=20）, the normal control group Ⅱ（n=12）, the hypertension model group Ⅰ （n=12）, the hypertension model group Ⅱ （n=12）, the YHR group （n=12） and the Captopril group （n=12）. The observation was carried out in the normal control group Ⅰ and the hypertension model groupⅠ after 4 weeks of modeling, and the other four groups were observed after 16 weeks of modeling （12 weeks of administration）. The cardiac function was measured with a multichannel biological signal analysis system, and the myocardium ultrastructure was observed by a transmission electron microscope. Results： （1） Compared with the normal control group Ⅰ, the systolic blood pressure and cardiac coefficient （left ventricular weight/body weight） in the model Ⅰ group was higher （P〈0.05, P〈0.01）. （2） In the YHR group, cardiac coefficient and -dp/dtmax were lower, left ventricular systolic pressure and ＋dp/dtmin were higher when compared with the model group Ⅱ and the Captopril group （P〈0.05 or P〈0.01）. In the Captopril group, only cardiac coefficient was lower when compared with the mode group Ⅱ （P〈0.05）. （3） Compared with the normal control group Ⅱ, ＋dp/dtmax was higher （P〈0.01）, -dp/dtmax and isovolumetric contraction time （ICT） was lower （P〈0.05, P〈0.01） in both the YHR group and the Captopril group. （4） Results of the myocardium ultrastructure showed edema under myocardium plasmalemma, enlarged sarcoplasmic reticulum and T tube, and significantly enlarged intercalated disc of the cardiac muscle in the model groups. In the Captopril group, the extension of s展开更多
基金This project was supported by the National Natural Science Foundation of China(No.81600317 and No.81700345)the Open Foundation of Hubei Key Laboratory of Biological Targeted Therapy(No.02.03.2014-10).
文摘Summary:Ranolazine,a late sodium current inhibitor,has been demonstrated to be effective on heart failure.18B-glycyrrhetinic acid(18β-GA)has the similar inhibitory effect on late sodium currents.However,its effect on diastolic function is still unknown.This study aimed to determine whether 18β-GA can improve the diastolic function and to explore the underlying mechanisms.Eighty male Sprague Dawley(SD)rats of Langendorff model were randomly divided into the following groups:group A,normal cardiac perfusion group;group B,ischemia-reperfusion group;group C,ischemia-reperfusion with anemoniasulcata toxinⅡ(ATX-Ⅱ);group D,ranolazine group;and group E,18β-GA group with four different concentrations.Furthermore,a pressure-overloaded rat model induced by trans-aortic constriction(TAC)was established.Echocardiography and hemodynamics were used to evaluate diastolic function at 14th day after TAC.Changes of free intracellular calcium(Ca27)concentration was indirectly detected by laser scanning confocal microscope to confirm the inhibition of late sodium currents.With the intervention of ATX-Ⅱon ischemia reperfusion injury group,5 umol/L ranolazine,and 5,10,20,40μmol/L 18β-GA could improve ATX-I-induced cardiac diastolic dysfunction.630 mg/kg glycyrrhizin tablets could improve cardiac diastolic function in the pressure-overloaded rats.18B-GA and ranolazine had similar effects on reducing the free calcium in cardiomyocytes.The study demonstrates that 18B-GA and glycyrrhizin could improve diastolic dysfunction induced by ischemia-reperfusion injury in Langendorff-perfused rat hearts and pressure-overloaded rats.The mechanism may be attributed to the inhibition of enhanced late sodium currents.
文摘目的探索接受血液透析的终末期肾病(end-stage renal disease,ESRD)患者液体状态和肺功能之间的关系。方法2019年4月~10月,纳入在辽阳市中心医院血液净化中心接受血液透析的ESRD患者54例。在透析前和透析后,测量患者的呼吸功能,同时采用电阻抗分析方法(bioimpedance analysis,BIA)测量患者的水负荷(overhydration,OH)和细胞外液(extracellular water,ECW),并计算2者的百分比(OH/ECW%)。OH/ECW%>7%视为液体超载。结果血液透析后用力肺活量(forced vital capacity,FVC)、FVC占预计量的百分比(forced vital capacity as a percentage of predicted normal value,FVC%)和第1秒的用力呼气容积(forced expiratory volume in one second,FEV_(1))水平显著升高,而OH/ECW%降低(t值分别为2.213、2.389、2.327、15.117,P值分别为0.030、0.019、0.022、<0.001)。透析前共有15例(27.8%)患者发生了液体超载。液体超载组的FVC、FVC%、FEV_(1)、FEV_(1)占预计量的百分比(FEV_(1)as a percentage of predicted normal value,FEV_(1)%)、用力呼出气量为25%~75%肺活量时的平均流量(forced expiratory flow between 25%and 75%of the FVC,FEF_(25-75))、呼出肺活量25%~75%时的用力呼气流量占预计量百分比(forced expiratory flow between 25%and 75%of the FVC as a percentage of predicted normal values,FEF_(25-75)%)、呼气峰值流速(peak expiratory flow rate,PEFR)、呼气峰值流速占预计量的百分比(peak expiratory flow rate as a percentage of predicted normal value,PEFR%)均低于无液体超载组(t值分别为4.740、5.131、4.902、6.226、4.263、3.668、2.904、3.694,P值分别为<0.001、<0.001、<0.001、<0.001、<0.001、<0.001、0.005、<0.001)。透析前OH/ECW%与FVC、FVC%、FEV_(1)、FEV_(1)%、FEF25–75、FEF25–75%、PEFR、PEFR%呈负相关(r值分别为–0.411、–0.530、–0.395、–0.491、–0.307、–0.272、–0.302、–0.425,P值分别为0.002、<0.001、0.003、<0.001、0.024、0.047、0.027、0.001)。多元线性回归分析模型显示,透析
基金Supported by Scientific Foundation of Medical College of the Chinese People's Armed Police Forces(No.WY2002-8)
文摘Objective： To investigate the effect of Yiqi Huoxue Recipe （YHR, 益气活血方） on the cardiac function and ultrastructure during the regression of myocardial hypertrophy induced by pressure overload in rats. Methods： The model of myocardial hypertrophy was established by abdominal aortic banding. Eighty male Wistar rats were divided into six groups, the normal control group Ⅰ （n=20）, the normal control group Ⅱ（n=12）, the hypertension model group Ⅰ （n=12）, the hypertension model group Ⅱ （n=12）, the YHR group （n=12） and the Captopril group （n=12）. The observation was carried out in the normal control group Ⅰ and the hypertension model groupⅠ after 4 weeks of modeling, and the other four groups were observed after 16 weeks of modeling （12 weeks of administration）. The cardiac function was measured with a multichannel biological signal analysis system, and the myocardium ultrastructure was observed by a transmission electron microscope. Results： （1） Compared with the normal control group Ⅰ, the systolic blood pressure and cardiac coefficient （left ventricular weight/body weight） in the model Ⅰ group was higher （P〈0.05, P〈0.01）. （2） In the YHR group, cardiac coefficient and -dp/dtmax were lower, left ventricular systolic pressure and ＋dp/dtmin were higher when compared with the model group Ⅱ and the Captopril group （P〈0.05 or P〈0.01）. In the Captopril group, only cardiac coefficient was lower when compared with the mode group Ⅱ （P〈0.05）. （3） Compared with the normal control group Ⅱ, ＋dp/dtmax was higher （P〈0.01）, -dp/dtmax and isovolumetric contraction time （ICT） was lower （P〈0.05, P〈0.01） in both the YHR group and the Captopril group. （4） Results of the myocardium ultrastructure showed edema under myocardium plasmalemma, enlarged sarcoplasmic reticulum and T tube, and significantly enlarged intercalated disc of the cardiac muscle in the model groups. In the Captopril group, the extension of s
