目的采用3.0 T MR IDEAL-IQ技术定量测量冈上肌脂肪分数(FF)值,并分析冈上肌FF值与冈上肌肌腱损伤程度(TTS)、年龄、性别、身体质量指数(BMI)以及症状持续时间的关系。方法选取157例有肩关节临床症状的患者或志愿者,进行肩关节MRI常规...目的采用3.0 T MR IDEAL-IQ技术定量测量冈上肌脂肪分数(FF)值,并分析冈上肌FF值与冈上肌肌腱损伤程度(TTS)、年龄、性别、身体质量指数(BMI)以及症状持续时间的关系。方法选取157例有肩关节临床症状的患者或志愿者,进行肩关节MRI常规序列扫描及IDEAL-IQ序列斜矢状位扫描。按照常规MRI上TTS表现分为正常组、退变组、部分撕裂组和完全撕裂组。通过后处理软件测量冈上肌FF值,并对冈上肌FF值与冈上肌TTS及受试者特征进行统计学分析。结果在冈上肌完全撕裂组、年龄≥51岁、BMI≥30 kg/m^2、女性和较长的症状持续时间的亚组中可以观察到较高的冈上肌FF值,冈上肌FF值与冈上肌TTS、年龄、性别及BMI之间存在相关性(r=0.84、0.59、0.21、0.34),但和症状持续时间无关(P>0.05)。结论IDEAL-IQ可以实现慢性冈上肌腱损伤中脂肪浸润的定量研究。展开更多
Background Preeclampsia, especially early onset of preeclampsia （PE）, is a common and serious disorder with high maternal and perinatal morbidity and mortality. Dietary factor is one of the most important factors wh...Background Preeclampsia, especially early onset of preeclampsia （PE）, is a common and serious disorder with high maternal and perinatal morbidity and mortality. Dietary factor is one of the most important factors which may affect the occurrence and development of the disease. The aim of this study is to investigate the effects of dietary factors on pathological changes of liver and placenta in preeclampsia-like mouse model by establishing the model at multiple stages of gestation. Methods Wild-type （WT） mice were injected subcutaneously with nitric oxide synthase （NOS） inhibitor L-arginine methyl ester （L-NAME, 50 mg·kg^-1·d^-1） to establish PE-like model （L-NAME group） at early-, mid-, and late- pregnant periods respectively; simultaneously, the control mice were injected with normal saline （NS group）. All the groups were divided into subgroups, standard chow group （SC）, and high-fat diet group （HF）. ApoE^-/- pregnant mice served as a control group. Systolic blood pressure （SBP）, urine protein, and histopathologic changes of placenta and liver in all groups were observed and statistically analyzed. Results In WT and apoE^-/- L-NAME subgroups, blood pressure and urine protein were significantly higher than those in all the gestational age matched NS groups （P 〈0.05）. Compared to other groups, remarkable liver fatty infiltration and lipid storage in placenta were found in early- and mid-L-NAME subgroups in apoE^-/- mice （P 〈0.05）, especially in the early- and mid-HF＋L-NAME subgroups in apoE^-/- mice （P 〈0.05）. More lipid storage droplets both in liver and placenta were found in ApoE^-/- mice than that of WT groups （P 〈0.05）. Morphology histopathologic examination of placentas showed varying degrees of fibrinoid necrosis and villous interstitial edema in early- and mid-L-NAME both in HF and SC of apoE^-/- and WT subgroups compared to NS controls （P 〈0.05）. But there was no significant difference between HF and SC subgroups （P 〉0.05）, and no展开更多
Electrospun fibers,with proven ability to promote tissue regeneration,are widely being explored for rotator cuff repairing.However,without post treatment,the microstructure of the electrospun scaffold is vastly differ...Electrospun fibers,with proven ability to promote tissue regeneration,are widely being explored for rotator cuff repairing.However,without post treatment,the microstructure of the electrospun scaffold is vastly different from that of natural extracellular matrix(ECM).Moreover,during mechanical loading,the nanofibers slip that hampers the proliferation and differentiation of migrating stem cells.Here,electrospun nanofiber scaffolds,with crimped nanofibers and welded joints to biomimic the intricate natural microstructure of tendon-to-bone insertion,were prepared using poly(ester-urethane)urea and gelatin via electrospinning and double crosslinking by a multi-bonding network densification strategy.The crimped nanofiber scaffold(CNS)features bionic tensile stress and induces chondrogenic differentiation,laying credible basis for in vivo experimentation.After repairing a rabbit massive rotator cuff tear using a CNS for 3 months,the continuous translational tendon-to-bone interface was fully regenerated,and fatty infiltration was simultaneously inhibited.Instead of micro-CT,μCT was employed to visualize the integrity and intricateness of the three-dimensional microstructure of the CNS-induced-healed tendon-to-bone interface at an ultra-high resolution of less than 1μm.This study sheds light on the correlation between nanofiber post treatment and massive rotator cuff repair and provides a general strategy for crimped nanofiber preparation and tendon-to-bone interface imaging characterization.展开更多
文摘目的采用3.0 T MR IDEAL-IQ技术定量测量冈上肌脂肪分数(FF)值,并分析冈上肌FF值与冈上肌肌腱损伤程度(TTS)、年龄、性别、身体质量指数(BMI)以及症状持续时间的关系。方法选取157例有肩关节临床症状的患者或志愿者,进行肩关节MRI常规序列扫描及IDEAL-IQ序列斜矢状位扫描。按照常规MRI上TTS表现分为正常组、退变组、部分撕裂组和完全撕裂组。通过后处理软件测量冈上肌FF值,并对冈上肌FF值与冈上肌TTS及受试者特征进行统计学分析。结果在冈上肌完全撕裂组、年龄≥51岁、BMI≥30 kg/m^2、女性和较长的症状持续时间的亚组中可以观察到较高的冈上肌FF值,冈上肌FF值与冈上肌TTS、年龄、性别及BMI之间存在相关性(r=0.84、0.59、0.21、0.34),但和症状持续时间无关(P>0.05)。结论IDEAL-IQ可以实现慢性冈上肌腱损伤中脂肪浸润的定量研究。
基金This work was supported by a grant from the National Natural Science Foundation of China （No. 30973204）.
文摘Background Preeclampsia, especially early onset of preeclampsia （PE）, is a common and serious disorder with high maternal and perinatal morbidity and mortality. Dietary factor is one of the most important factors which may affect the occurrence and development of the disease. The aim of this study is to investigate the effects of dietary factors on pathological changes of liver and placenta in preeclampsia-like mouse model by establishing the model at multiple stages of gestation. Methods Wild-type （WT） mice were injected subcutaneously with nitric oxide synthase （NOS） inhibitor L-arginine methyl ester （L-NAME, 50 mg·kg^-1·d^-1） to establish PE-like model （L-NAME group） at early-, mid-, and late- pregnant periods respectively; simultaneously, the control mice were injected with normal saline （NS group）. All the groups were divided into subgroups, standard chow group （SC）, and high-fat diet group （HF）. ApoE^-/- pregnant mice served as a control group. Systolic blood pressure （SBP）, urine protein, and histopathologic changes of placenta and liver in all groups were observed and statistically analyzed. Results In WT and apoE^-/- L-NAME subgroups, blood pressure and urine protein were significantly higher than those in all the gestational age matched NS groups （P 〈0.05）. Compared to other groups, remarkable liver fatty infiltration and lipid storage in placenta were found in early- and mid-L-NAME subgroups in apoE^-/- mice （P 〈0.05）, especially in the early- and mid-HF＋L-NAME subgroups in apoE^-/- mice （P 〈0.05）. More lipid storage droplets both in liver and placenta were found in ApoE^-/- mice than that of WT groups （P 〈0.05）. Morphology histopathologic examination of placentas showed varying degrees of fibrinoid necrosis and villous interstitial edema in early- and mid-L-NAME both in HF and SC of apoE^-/- and WT subgroups compared to NS controls （P 〈0.05）. But there was no significant difference between HF and SC subgroups （P 〉0.05）, and no
基金supported by Instrumental Analysis Center of Shanghai Jiao Tong University.This work was supported by the National Natural Science Foundation of China[Grant No.81902186,81671920,31972923,81871753,81772341]National Key Research and Development Program of China[Grant No.2018YFC1106200,2018YFC1106201,2018YFC1106202]Technology Support Project of Science and Technology Commission of Shanghai Municipality of China[Grant No.19441901700,19441901701,19441901702,18441902800].
文摘Electrospun fibers,with proven ability to promote tissue regeneration,are widely being explored for rotator cuff repairing.However,without post treatment,the microstructure of the electrospun scaffold is vastly different from that of natural extracellular matrix(ECM).Moreover,during mechanical loading,the nanofibers slip that hampers the proliferation and differentiation of migrating stem cells.Here,electrospun nanofiber scaffolds,with crimped nanofibers and welded joints to biomimic the intricate natural microstructure of tendon-to-bone insertion,were prepared using poly(ester-urethane)urea and gelatin via electrospinning and double crosslinking by a multi-bonding network densification strategy.The crimped nanofiber scaffold(CNS)features bionic tensile stress and induces chondrogenic differentiation,laying credible basis for in vivo experimentation.After repairing a rabbit massive rotator cuff tear using a CNS for 3 months,the continuous translational tendon-to-bone interface was fully regenerated,and fatty infiltration was simultaneously inhibited.Instead of micro-CT,μCT was employed to visualize the integrity and intricateness of the three-dimensional microstructure of the CNS-induced-healed tendon-to-bone interface at an ultra-high resolution of less than 1μm.This study sheds light on the correlation between nanofiber post treatment and massive rotator cuff repair and provides a general strategy for crimped nanofiber preparation and tendon-to-bone interface imaging characterization.
