Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male st...Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.展开更多
Postmenopausal osteoporosis （PMO） is considered a polygenic disease. The estrogen receptor β （ESR2） gene is a candidate mediating the genetic influence on bone mass and the risk of osteoporosis. The aim of this s...Postmenopausal osteoporosis （PMO） is considered a polygenic disease. The estrogen receptor β （ESR2） gene is a candidate mediating the genetic influence on bone mass and the risk of osteoporosis. The aim of this study is to investigate the association of a cytosine-adenine （CA） repeat polymorphism in the fifth intron of the ESR2 gene with PMO in Chinese Han population. The CA repeat polymorphism was genotyped in a case-control study, involving 78 femoral neck PMO patients vs. 122 controls and 108 lumbar spine （L2-4） PMO patients vs. 92 controls. The （CA）n〈22 and （CA）n≥22 alleles were designated short （S） and long （L）, respectively. ESR2 genotype was categorically defined as SS （2 S alleles）, SL （having the mixed S and L alleles）, and LL （2 L alleles）. At both the femoral neck and the L2-4 region, LL genotype and L allele frequencies of the PMO group were significantly higher than those of the control group （P〈0.01）. The subjects with the SL, the LL, and the combined SL and LL genotype had a significant increased risk of PMO when compared with those with the SS genotype （P〈0.05）. After adjustments for age, years since menopause, menopausal age, and body mass index, logistic regression analysis showed that the subjects with the combined SL and LL genotype had increased risk of PMO when compared with those with the SS genotype both at the femoral neck （adjusted OR 4.923, 95% CI 1.986-12.203 , P=0.001） and the L2-4 （adjusted OR 2.267, 95% CI 1.121-4.598, P=0.023）. This extensive association study has identified the ESR2 CA repeat polymorphism to be independently associated with PMO at the femoral neck and the L2-4 in Chinese Han population. The data also suggested that the presence of the L allele may dominantly increase the risk of PMO at the two regions.展开更多
Bisphenol A(BPA),as an environmental endocrine disruptor,affects human health by interfering with hormone secretion in the body.Endometriosis(EMs)is an estrogendependent disease that is influenced by a variety of fac...Bisphenol A(BPA),as an environmental endocrine disruptor,affects human health by interfering with hormone secretion in the body.Endometriosis(EMs)is an estrogendependent disease that is influenced by a variety of factors such as genetics,oxidative stress,immunity,and environment for its onset.In recent years,special attention has been paid to the influence of genetic polymorphisms on the pathogenesis of EMs.A search of the Comparative Toxicology Database revealed that BPA can act on a variety of different genes to induce disease development by regulating gene expression and that some genes play equally important roles in the development of EMs.Among them,we selected four genes(EGFR,ESR2,FOS,KRAS)in the estrogen signaling pathway,and now we review the mechanism of BPA acting on these four genes in different ways and the role of these four genes in the development of EMs,which will provide a reference for the subsequent study of the mechanism of BPA involvement in the development of EMs.展开更多
为探究绵羊ESR2基因多态性与产羔性状的相关性,本研究以杜泊羊、滩寒羊、杂一代、杂二代和横交一代5个绵羊群体为研究对象,利用Sequenom Mass ARRAY?SNP技术对5个绵羊群体ESR2基因3个错义突变位点进行检测,并与其产羔数进行关联分析。...为探究绵羊ESR2基因多态性与产羔性状的相关性,本研究以杜泊羊、滩寒羊、杂一代、杂二代和横交一代5个绵羊群体为研究对象,利用Sequenom Mass ARRAY?SNP技术对5个绵羊群体ESR2基因3个错义突变位点进行检测,并与其产羔数进行关联分析。结果显示,ESR2基因g.73323973C>T和g.73326795G>A位点在5个群体中均表现为中度多态(0.25<PIC<0.50);g.73353489C>A位点在滩寒羊群体中表现为低度多态(PIC<0.25),在杜泊羊、杂一代、杂二代和横交一代4个群体中表现为中度多态。产羔数关联分析表明,g.73323973C>T位点在滩寒羊群体中TT型产羔数极显著高于TC、CC型(P<0.01),说明g.73323973C>T位点适用于滩寒羊群体多羔性状的选育。通过生物信息学分析表明,3个位点突变前后ESR2蛋白的二级结构类型均发生了变化;蛋白互作网络分析表明ESR2蛋白与调控动物繁殖的相关蛋白质互作。本研究为开展提高绵羊繁殖力的分子标记辅助选育提供依据。展开更多
基金support from the National Key Research and Development Program of China(2022YFD1602000)the National Natural Science Foundation of China(32202514,U22A20498 and 32072596)+2 种基金the Joint Fund of Henan Province Science and Technology Research and Development Plan,China(222103810009)the Science and Technology Innovation Team of Shaanxi,China(2021TD-32)the China Postdoctoral Science Foundation(2022M711064 and 2023M741062).
文摘Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.
文摘Postmenopausal osteoporosis （PMO） is considered a polygenic disease. The estrogen receptor β （ESR2） gene is a candidate mediating the genetic influence on bone mass and the risk of osteoporosis. The aim of this study is to investigate the association of a cytosine-adenine （CA） repeat polymorphism in the fifth intron of the ESR2 gene with PMO in Chinese Han population. The CA repeat polymorphism was genotyped in a case-control study, involving 78 femoral neck PMO patients vs. 122 controls and 108 lumbar spine （L2-4） PMO patients vs. 92 controls. The （CA）n〈22 and （CA）n≥22 alleles were designated short （S） and long （L）, respectively. ESR2 genotype was categorically defined as SS （2 S alleles）, SL （having the mixed S and L alleles）, and LL （2 L alleles）. At both the femoral neck and the L2-4 region, LL genotype and L allele frequencies of the PMO group were significantly higher than those of the control group （P〈0.01）. The subjects with the SL, the LL, and the combined SL and LL genotype had a significant increased risk of PMO when compared with those with the SS genotype （P〈0.05）. After adjustments for age, years since menopause, menopausal age, and body mass index, logistic regression analysis showed that the subjects with the combined SL and LL genotype had increased risk of PMO when compared with those with the SS genotype both at the femoral neck （adjusted OR 4.923, 95% CI 1.986-12.203 , P=0.001） and the L2-4 （adjusted OR 2.267, 95% CI 1.121-4.598, P=0.023）. This extensive association study has identified the ESR2 CA repeat polymorphism to be independently associated with PMO at the femoral neck and the L2-4 in Chinese Han population. The data also suggested that the presence of the L allele may dominantly increase the risk of PMO at the two regions.
文摘Bisphenol A(BPA),as an environmental endocrine disruptor,affects human health by interfering with hormone secretion in the body.Endometriosis(EMs)is an estrogendependent disease that is influenced by a variety of factors such as genetics,oxidative stress,immunity,and environment for its onset.In recent years,special attention has been paid to the influence of genetic polymorphisms on the pathogenesis of EMs.A search of the Comparative Toxicology Database revealed that BPA can act on a variety of different genes to induce disease development by regulating gene expression and that some genes play equally important roles in the development of EMs.Among them,we selected four genes(EGFR,ESR2,FOS,KRAS)in the estrogen signaling pathway,and now we review the mechanism of BPA acting on these four genes in different ways and the role of these four genes in the development of EMs,which will provide a reference for the subsequent study of the mechanism of BPA involvement in the development of EMs.
文摘为探究绵羊ESR2基因多态性与产羔性状的相关性,本研究以杜泊羊、滩寒羊、杂一代、杂二代和横交一代5个绵羊群体为研究对象,利用Sequenom Mass ARRAY?SNP技术对5个绵羊群体ESR2基因3个错义突变位点进行检测,并与其产羔数进行关联分析。结果显示,ESR2基因g.73323973C>T和g.73326795G>A位点在5个群体中均表现为中度多态(0.25<PIC<0.50);g.73353489C>A位点在滩寒羊群体中表现为低度多态(PIC<0.25),在杜泊羊、杂一代、杂二代和横交一代4个群体中表现为中度多态。产羔数关联分析表明,g.73323973C>T位点在滩寒羊群体中TT型产羔数极显著高于TC、CC型(P<0.01),说明g.73323973C>T位点适用于滩寒羊群体多羔性状的选育。通过生物信息学分析表明,3个位点突变前后ESR2蛋白的二级结构类型均发生了变化;蛋白互作网络分析表明ESR2蛋白与调控动物繁殖的相关蛋白质互作。本研究为开展提高绵羊繁殖力的分子标记辅助选育提供依据。
