利用超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳技术,对国家枇杷种质资源圃所收集保存的113个枇杷(E riobotrya jap on ica L ind l.)品种(或株系)和4个野生近缘种[栎叶枇杷(E.p rinoid es R ehd.etW ils.)、大渡河枇杷(E.p rinoid es v...利用超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳技术,对国家枇杷种质资源圃所收集保存的113个枇杷(E riobotrya jap on ica L ind l.)品种(或株系)和4个野生近缘种[栎叶枇杷(E.p rinoid es R ehd.etW ils.)、大渡河枇杷(E.p rinoid es var.d aduheensis H.Z.Zhang)、齿叶枇杷(E.serra ta V ida l.)、大瑶山枇杷(E.d ayaoshanensisChen.)],共117份材料进行了等位酶遗传变异分析。在12个酶系中共检测到24个清晰位点和59个等位基因,多态位点为21个,位点最大等位基因数为5,体现出枇杷丰富的遗传种质多样性;χ2分析表明等位基因频率在不同产地品种群间存在明显差异,在用于分析的19个多态位点中,有15个位点达到显著或极显著水平;且不同的种材料拥有各自特有等位基因,如D ia-1c,D ia-2b,D ia-3b只存在于大瑶山枇杷中,E st-2b,E st-3a只存在于大渡河枇杷中,Idh-1d仅出现于枇杷品种荔枝枇杷中,体现了枇杷物种间的遗传组成差异;利用11个酶系统22个位点的53个等位基因所构建的枇杷品种(株系)等位酶基因型指纹可以将113个枇杷品种中的111个完全区分,各品种均有自己独特的等位酶基因型指纹,虽然进一步的分析表明,目前所研究的酶系统位点和等位基因变异与枇杷品种果实园艺性状变异间缺乏关联,但等位酶标记仍然不失为枇杷品种鉴别的一种有用工具。展开更多
Accumulation of carotenoids in peel and pulp of the yellow-fleshe loquat ‘Zaozhong 6'(ZZ6) and the white-fleshe loquat ‘Baiyu'(BY) were tracked during different fruit development stages, and the expression o...Accumulation of carotenoids in peel and pulp of the yellow-fleshe loquat ‘Zaozhong 6'(ZZ6) and the white-fleshe loquat ‘Baiyu'(BY) were tracked during different fruit development stages, and the expression of 15 carotenogenic genes were analyzed. During loquat fruit ripening the fresh weight content of β-carotene in peel and pulp of ZZ6 increased gradually and peaked at the fully ripe stage, reaching 68.53 μg·g^(-1)FW in the peel and 11.92 μg·g^(-1)FW in the pulp. In BY, the content of β-carotene in the peel increased and peaked at the fully ripe stage, reaching38.89 μg·g^(-1)FW, while it decreased in the pulp from the original 0.47 μg·g~(^(-1))FW and reduced to 0.29 μg·g~(^(-1))FW. The content of β-cryptoxanthin in the peel and pulp of ZZ6 and BY both increased steadily, and peaked at the fully ripe stage; however, the content of lutein decreased in the peel of ZZ6 and increased in the pulp, but in BY, it dropped and then rose in the peel. There was no significan change of β-cryptoxanthin in the pulp of BY. After the breaker stage, the m RNA levels of phytoene synthase(PSY) and chromoplast-specifi lycopene β-cyclase(CYCB) were higher in the peel, while CYCB and β-carotene hydroxylase(BCH) m RNAs were higher in the fles of ZZ6, compared with BY. The results showed that the expression level of PSY, CYCB, and BCH appeared to cooperatively regulate the accumulation of carotenoids.展开更多
The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the lengt...The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.展开更多
Relationships between plant water status and gas exchange parameters at increasing levels of water stress were determined in Algerie loquats which grown in 50 I pots. Changes in soil water content and stem water poten...Relationships between plant water status and gas exchange parameters at increasing levels of water stress were determined in Algerie loquats which grown in 50 I pots. Changes in soil water content and stem water potential and their effects on stomatal conductance （Gs ） and net photosynthesis （Pn） rate were followed in control plants and in plants without irrigation until the latter reached near permanent wilting point and some leaf abscission took place. Then, the irrigation was restarted and the comparison repeated. Soil water content and stem water potential gradually diminished in response to drought reaching the minimum values of 0.9 mm and -5.0 MPa, respectively, 9 days after watering suspension. Compromised plant water status had drastic effects on Gs values that dropped by 97％ in the last day of the drought period. Pn was diminished by 80％ at the end of the drought period. The increasing levels of water stress did not cause a steady increase in leaf temperature in non-irrigated plants. Non-irrigated plants wilted and lost some leaves due to the severity of the water stress. However, all non-irrigated plants survived and reached similar Pn than control plants just a week after the irrigation was restarted, confirming drought tolerance of loquat and suggesting that photosynthesis machinery remained intact.展开更多
文摘利用超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳技术,对国家枇杷种质资源圃所收集保存的113个枇杷(E riobotrya jap on ica L ind l.)品种(或株系)和4个野生近缘种[栎叶枇杷(E.p rinoid es R ehd.etW ils.)、大渡河枇杷(E.p rinoid es var.d aduheensis H.Z.Zhang)、齿叶枇杷(E.serra ta V ida l.)、大瑶山枇杷(E.d ayaoshanensisChen.)],共117份材料进行了等位酶遗传变异分析。在12个酶系中共检测到24个清晰位点和59个等位基因,多态位点为21个,位点最大等位基因数为5,体现出枇杷丰富的遗传种质多样性;χ2分析表明等位基因频率在不同产地品种群间存在明显差异,在用于分析的19个多态位点中,有15个位点达到显著或极显著水平;且不同的种材料拥有各自特有等位基因,如D ia-1c,D ia-2b,D ia-3b只存在于大瑶山枇杷中,E st-2b,E st-3a只存在于大渡河枇杷中,Idh-1d仅出现于枇杷品种荔枝枇杷中,体现了枇杷物种间的遗传组成差异;利用11个酶系统22个位点的53个等位基因所构建的枇杷品种(株系)等位酶基因型指纹可以将113个枇杷品种中的111个完全区分,各品种均有自己独特的等位酶基因型指纹,虽然进一步的分析表明,目前所研究的酶系统位点和等位基因变异与枇杷品种果实园艺性状变异间缺乏关联,但等位酶标记仍然不失为枇杷品种鉴别的一种有用工具。
基金partially supported by Guangzhou Science and Technology Program key projects(201504010028)
文摘Accumulation of carotenoids in peel and pulp of the yellow-fleshe loquat ‘Zaozhong 6'(ZZ6) and the white-fleshe loquat ‘Baiyu'(BY) were tracked during different fruit development stages, and the expression of 15 carotenogenic genes were analyzed. During loquat fruit ripening the fresh weight content of β-carotene in peel and pulp of ZZ6 increased gradually and peaked at the fully ripe stage, reaching 68.53 μg·g^(-1)FW in the peel and 11.92 μg·g^(-1)FW in the pulp. In BY, the content of β-carotene in the peel increased and peaked at the fully ripe stage, reaching38.89 μg·g^(-1)FW, while it decreased in the pulp from the original 0.47 μg·g~(^(-1))FW and reduced to 0.29 μg·g~(^(-1))FW. The content of β-cryptoxanthin in the peel and pulp of ZZ6 and BY both increased steadily, and peaked at the fully ripe stage; however, the content of lutein decreased in the peel of ZZ6 and increased in the pulp, but in BY, it dropped and then rose in the peel. There was no significan change of β-cryptoxanthin in the pulp of BY. After the breaker stage, the m RNA levels of phytoene synthase(PSY) and chromoplast-specifi lycopene β-cyclase(CYCB) were higher in the peel, while CYCB and β-carotene hydroxylase(BCH) m RNAs were higher in the fles of ZZ6, compared with BY. The results showed that the expression level of PSY, CYCB, and BCH appeared to cooperatively regulate the accumulation of carotenoids.
文摘The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.
基金partially financed by the Junta de Andalucía with European Union(FEDER)funds(AGR-03183)
文摘Relationships between plant water status and gas exchange parameters at increasing levels of water stress were determined in Algerie loquats which grown in 50 I pots. Changes in soil water content and stem water potential and their effects on stomatal conductance （Gs ） and net photosynthesis （Pn） rate were followed in control plants and in plants without irrigation until the latter reached near permanent wilting point and some leaf abscission took place. Then, the irrigation was restarted and the comparison repeated. Soil water content and stem water potential gradually diminished in response to drought reaching the minimum values of 0.9 mm and -5.0 MPa, respectively, 9 days after watering suspension. Compromised plant water status had drastic effects on Gs values that dropped by 97％ in the last day of the drought period. Pn was diminished by 80％ at the end of the drought period. The increasing levels of water stress did not cause a steady increase in leaf temperature in non-irrigated plants. Non-irrigated plants wilted and lost some leaves due to the severity of the water stress. However, all non-irrigated plants survived and reached similar Pn than control plants just a week after the irrigation was restarted, confirming drought tolerance of loquat and suggesting that photosynthesis machinery remained intact.
