采用酶解辅助热水浸提法用乙醇提取山药多糖,考察了乙醇体积分数、提取温度、固液比与提取时间对提取量的影响。结果表明,山药多糖的最佳提取工艺为:乙醇体积分数为70%,提取温度90℃,固液比1∶40,提取时间60 m in。在此条件下,山药多糖...采用酶解辅助热水浸提法用乙醇提取山药多糖,考察了乙醇体积分数、提取温度、固液比与提取时间对提取量的影响。结果表明,山药多糖的最佳提取工艺为:乙醇体积分数为70%,提取温度90℃,固液比1∶40,提取时间60 m in。在此条件下,山药多糖的提取量为4.76 mg/g。展开更多
Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L_9（3~4） orthogonal array design and separation effect of cation exchange resin on galanthamine...Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L_9（3~4） orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of p H value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows： ratio of solid to liquid（g：mL） 1：10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50oC, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine.展开更多
Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due ...Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due to its ability to evade the host's defense mechanisms,early tracking of HTLV-1 becomes crucial.In this study,we integrateλ-Exonuclease(λ-Exo)-assisted target recycling with a terminal deoxynucleotidyl transferase(TdT)-mediated template-free DNA extension process to develop an electrochemical analysis platform for the specific and sensitive detection of HTLV-1 DNA.During theλ-Exo-assisted target recycling,HTLV-1 DNA is recognized by hairpin DNA(Hp-DNA),forming double-stranded DNA(dsDNA)through DNA hybridization.The dsDNA,featuring blunt 5'terminal phosphorylation,is cleaved byλ-Exo,generating abundant short output sequence(sDNA).HTLV-1 DNA is released,initiating a cyclic hybridization-cleavage process.Subsequently,thiol-labelled capture DNA(CP-DNA)assembled on gold electrode surface captures a substantial amount of the generated sDNA,forming CP-DNA-sDNA nanostructures.When TdT and dNTPs are present on the electrode surface,the 3'-OH terminal of sDNA extends to generate long single-stranded DNA(ssDNA)structure.Methylene blue(MB)is selected as the electrochemical signal molecule.MB not only binds with ssDNA but also interacts specifically with dsDNA,resulting in a significantly enhanced electrochemical signal on modified electrode surface.The detection limit of HTLV-1 DNA is as low as 19 amol/L(S/N=3)when the two signal amplification strategies are combined.The analysis platform exhibits excellent analytical performance and holds promise as a novel tool for the early tracing and diagnosis of HTLV-1 DNA.展开更多
A novel method using ethanol and ultrasound to extract oil from cream obtained from enzyme-assisted aqueous extraction of soybean oil was developed.To evaluate the relationships between operating variables and free oi...A novel method using ethanol and ultrasound to extract oil from cream obtained from enzyme-assisted aqueous extraction of soybean oil was developed.To evaluate the relationships between operating variables and free oil yield and to maximize the free oil yield,response surface methodology was introduced in this work.The developed regression model was fitted with R2=0.9591.Optimized variables were:ethanol concentration of73%,ethanol addition volume of 0.55 L/kg,ultrasound power of 427 W,ultrasound time of 47 s,and ultrasound temperature of 53℃.The free oil yield from the cream under the above conditions was 92.6±3.4%.Scanning electron microscopy(SEM)was used to evaluate the effect of ultrasonic treatment on ethanoltreated cream,and the SEM images clearly showed that the ultrasound treatment affected dispersing and fracturing of the microstructure of ethanol-treated cream.展开更多
基金Hunan Provincial Science and Technology Department(2012TP4002-3)Zhangjiajie Science and Technology Bureau(2014YB17)
文摘Objective To explore the optimum condition for complex enzyme-assisted extraction of galanthamine from Lycoris aurea by L_9（3~4） orthogonal array design and separation effect of cation exchange resin on galanthamine. Methods Cellulase and pectinase solution was used as the extraction solvent. The effects of p H value of enzyme, amount of complex enzyme, dissociation time, and enzymatic hydrolysis temperature on the extraction results were investigated. Results The optimal conditions were obtained as follows： ratio of solid to liquid（g：mL） 1：10, pH value 4.5, amount of complex enzymes 4%, enzymatic hydrolysis temperature 50oC, and reaction time 2.0 h. Under these conditions, the extraction yield of galanthamine was 0.0294%. In addition, D-001 cation exchange resin was selected for separation of galanthamine. The separation conditions were that adsorption flow rate was 3 BV/h with reagent of pH2 and the desorption flow rate was 3 BV/h with 70% ethanol solution containing 1.5 mol/L ammonia. After separation, the content of galanthamine was increased to 12.31%. Conclusion The results provide a reference for industrial production of galanthamine.
基金financially supported by Central Leading Local Science and Technology Development Fund Project(guikeZY22096017)Natural Science Foundation of Guangxi Province(2024GXNSFDA010036)National Natural Science Foundation of China(22164014,U23A2089).
文摘Human T-cell lymphophilic virus type 1(HTLV-1),the known retrovirus causing cancer in humans,is closely associated with adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis.Due to its ability to evade the host's defense mechanisms,early tracking of HTLV-1 becomes crucial.In this study,we integrateλ-Exonuclease(λ-Exo)-assisted target recycling with a terminal deoxynucleotidyl transferase(TdT)-mediated template-free DNA extension process to develop an electrochemical analysis platform for the specific and sensitive detection of HTLV-1 DNA.During theλ-Exo-assisted target recycling,HTLV-1 DNA is recognized by hairpin DNA(Hp-DNA),forming double-stranded DNA(dsDNA)through DNA hybridization.The dsDNA,featuring blunt 5'terminal phosphorylation,is cleaved byλ-Exo,generating abundant short output sequence(sDNA).HTLV-1 DNA is released,initiating a cyclic hybridization-cleavage process.Subsequently,thiol-labelled capture DNA(CP-DNA)assembled on gold electrode surface captures a substantial amount of the generated sDNA,forming CP-DNA-sDNA nanostructures.When TdT and dNTPs are present on the electrode surface,the 3'-OH terminal of sDNA extends to generate long single-stranded DNA(ssDNA)structure.Methylene blue(MB)is selected as the electrochemical signal molecule.MB not only binds with ssDNA but also interacts specifically with dsDNA,resulting in a significantly enhanced electrochemical signal on modified electrode surface.The detection limit of HTLV-1 DNA is as low as 19 amol/L(S/N=3)when the two signal amplification strategies are combined.The analysis platform exhibits excellent analytical performance and holds promise as a novel tool for the early tracing and diagnosis of HTLV-1 DNA.
基金the National High-tech R&D Program of China(863 Program)(grant number 2013AA102104)the open-end fund from the Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricultural University(grant numberSB12C01)+1 种基金the Special Fund for the Establishment of Modern Agricultural R&D Systems(grant number nycytx-004)the National Research Center of Soybean Engineering and Technology,and the Northeast Agricultural University for the support of this project
文摘A novel method using ethanol and ultrasound to extract oil from cream obtained from enzyme-assisted aqueous extraction of soybean oil was developed.To evaluate the relationships between operating variables and free oil yield and to maximize the free oil yield,response surface methodology was introduced in this work.The developed regression model was fitted with R2=0.9591.Optimized variables were:ethanol concentration of73%,ethanol addition volume of 0.55 L/kg,ultrasound power of 427 W,ultrasound time of 47 s,and ultrasound temperature of 53℃.The free oil yield from the cream under the above conditions was 92.6±3.4%.Scanning electron microscopy(SEM)was used to evaluate the effect of ultrasonic treatment on ethanoltreated cream,and the SEM images clearly showed that the ultrasound treatment affected dispersing and fracturing of the microstructure of ethanol-treated cream.
