以中国北方沿海14种经济鱼类(日本下鱵鱼、斑尾复鰕虎鱼、黄尾鰤、鳙鱼、大泷六线鱼、绿鳍马面鲀、大菱鲆、日本笠鳚、绒杜父鱼、牙鲆、斑纹条鳎、长吻红舌鳎、方式云鳚、大西洋鳕)和7种鲽鱼(粗壮拟庸鲽鱼、黄盖鲽、亚洲箭齿鲽、星斑川...以中国北方沿海14种经济鱼类(日本下鱵鱼、斑尾复鰕虎鱼、黄尾鰤、鳙鱼、大泷六线鱼、绿鳍马面鲀、大菱鲆、日本笠鳚、绒杜父鱼、牙鲆、斑纹条鳎、长吻红舌鳎、方式云鳚、大西洋鳕)和7种鲽鱼(粗壮拟庸鲽鱼、黄盖鲽、亚洲箭齿鲽、星斑川鲽鱼、马舌鲽、木叶鲽、石鲽)为研究对象,首先对其线粒体16S r RNA基因片段进行PCR扩增及序列同源性比对,随后采用Dde I、Hae III和Nla III 3种限制性内切酶对21种鱼类的细胞色素b基因序列进行限制性片段长度多态性聚合酶链反应(PCR-RFLP)分析,并利用芯片生物分析技术得到酶切产物的特异性图谱和确切片段大小,结果共检出132个电泳条带,实现了21种鱼类的有效鉴别。本文为鱼类种类鉴定和鱼类食品检测提供了科学依据。展开更多
Concentrations of lead( Pb),cadmium( Cd),chromium( Cr),copper( Cu),zinc( Zn) and manganese( Mn) were measured in various organs( such as in liver and muscle) from 9 species of freshwater economic fishes which were col...Concentrations of lead( Pb),cadmium( Cd),chromium( Cr),copper( Cu),zinc( Zn) and manganese( Mn) were measured in various organs( such as in liver and muscle) from 9 species of freshwater economic fishes which were collected from northeast area of Guangdong Province. The concentration of metals was measured by inductively coupled plasma atomic emission spectrometry( ICP- AES). Results showed that the levels of metals in hepatopancreas of the fishes were found in order of Zn > Pb > Cu > Hg > Cd,while in muscles were Zn > Cr> Pb > Mn > Cu > Cd. In general,the metals concentrations were significantly higher in liver samples than that in muscle samples. Based on the " pollution index of single factor",the fishes,to one degree or another,were polluted by Pb,Cd,Cr,Cu and Zn,and pollution levels were mostly followed by Pb > Cd > Cr > Cu > Zn. The indexes of Pb and Cd tested in hepatopancreas of the fishes were in a majority exceeded the national safety criteria for food in China. What's more,it was found that the contents of the heavy metals in fishes did not vary with the trophic level which they belong to. In summary,the fishes were polluted by Pb,Cd,Cr,Cu and Zn to some extent,which indicated that hidden danger of heavy metals pollution was present in ecological environment or safety in fishery production in the area.展开更多
文摘以中国北方沿海14种经济鱼类(日本下鱵鱼、斑尾复鰕虎鱼、黄尾鰤、鳙鱼、大泷六线鱼、绿鳍马面鲀、大菱鲆、日本笠鳚、绒杜父鱼、牙鲆、斑纹条鳎、长吻红舌鳎、方式云鳚、大西洋鳕)和7种鲽鱼(粗壮拟庸鲽鱼、黄盖鲽、亚洲箭齿鲽、星斑川鲽鱼、马舌鲽、木叶鲽、石鲽)为研究对象,首先对其线粒体16S r RNA基因片段进行PCR扩增及序列同源性比对,随后采用Dde I、Hae III和Nla III 3种限制性内切酶对21种鱼类的细胞色素b基因序列进行限制性片段长度多态性聚合酶链反应(PCR-RFLP)分析,并利用芯片生物分析技术得到酶切产物的特异性图谱和确切片段大小,结果共检出132个电泳条带,实现了21种鱼类的有效鉴别。本文为鱼类种类鉴定和鱼类食品检测提供了科学依据。
基金Supported by Natural Science Foundation of Guangdong(S2013010013693)Outstanding Young Teacher Training Program of Colleges and Universities in Guangdong Province(Yq2013152)
文摘Concentrations of lead( Pb),cadmium( Cd),chromium( Cr),copper( Cu),zinc( Zn) and manganese( Mn) were measured in various organs( such as in liver and muscle) from 9 species of freshwater economic fishes which were collected from northeast area of Guangdong Province. The concentration of metals was measured by inductively coupled plasma atomic emission spectrometry( ICP- AES). Results showed that the levels of metals in hepatopancreas of the fishes were found in order of Zn > Pb > Cu > Hg > Cd,while in muscles were Zn > Cr> Pb > Mn > Cu > Cd. In general,the metals concentrations were significantly higher in liver samples than that in muscle samples. Based on the " pollution index of single factor",the fishes,to one degree or another,were polluted by Pb,Cd,Cr,Cu and Zn,and pollution levels were mostly followed by Pb > Cd > Cr > Cu > Zn. The indexes of Pb and Cd tested in hepatopancreas of the fishes were in a majority exceeded the national safety criteria for food in China. What's more,it was found that the contents of the heavy metals in fishes did not vary with the trophic level which they belong to. In summary,the fishes were polluted by Pb,Cd,Cr,Cu and Zn to some extent,which indicated that hidden danger of heavy metals pollution was present in ecological environment or safety in fishery production in the area.
