The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora(DPC) and D. phaseolorum var.meridionalis (DPM). The primers and TaqMan MGB probes were designed based...The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora(DPC) and D. phaseolorum var.meridionalis (DPM). The primers and TaqMan MGB probes were designed based on the ITS of DPC, DPM, D. phaseolorum var. sojae and Phomopsis longicolla. A series of genomic DNA dilution were used to detect sensitivity of the technique, the results showed that the limits of detection for DPM and DPC were 7 fg/μL and 6 fg/μL DNA respectively.展开更多
Brown rots of Red Fuji apples were observed in Hangzhou city (Zhengjiang Province, China). The causal agent was isolated and identified in both morphological and molecular genetic levels. The phenotype and phylogeneti...Brown rots of Red Fuji apples were observed in Hangzhou city (Zhengjiang Province, China). The causal agent was isolated and identified in both morphological and molecular genetic levels. The phenotype and phylogenetic analysis revealed that the isolate was </span><i><span style="font-family:Verdana;">Diaporthe phaseolorum</span></i><span style="font-family:Verdana;"> var. </span><i><span style="font-family:Verdana;">caulivora</span></i><span style="font-family:Verdana;">, and its pathogenicity on apple fruit was confirmed by re-inoculation experiment. To our knowledge, this is the first report of </span><i><span style="font-family:Verdana;">D. phaseolorum</span></i><span style="font-family:Verdana;"> var. </span><i><span style="font-family:Verdana;">caulivora</span></i><span style="font-family:Verdana;"> causing postharvest fruit rot on apple in China.展开更多
文摘The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora(DPC) and D. phaseolorum var.meridionalis (DPM). The primers and TaqMan MGB probes were designed based on the ITS of DPC, DPM, D. phaseolorum var. sojae and Phomopsis longicolla. A series of genomic DNA dilution were used to detect sensitivity of the technique, the results showed that the limits of detection for DPM and DPC were 7 fg/μL and 6 fg/μL DNA respectively.
文摘Brown rots of Red Fuji apples were observed in Hangzhou city (Zhengjiang Province, China). The causal agent was isolated and identified in both morphological and molecular genetic levels. The phenotype and phylogenetic analysis revealed that the isolate was </span><i><span style="font-family:Verdana;">Diaporthe phaseolorum</span></i><span style="font-family:Verdana;"> var. </span><i><span style="font-family:Verdana;">caulivora</span></i><span style="font-family:Verdana;">, and its pathogenicity on apple fruit was confirmed by re-inoculation experiment. To our knowledge, this is the first report of </span><i><span style="font-family:Verdana;">D. phaseolorum</span></i><span style="font-family:Verdana;"> var. </span><i><span style="font-family:Verdana;">caulivora</span></i><span style="font-family:Verdana;"> causing postharvest fruit rot on apple in China.
