为了挖掘陆地棉可能与耐盐性相关的功能SNP,本实验以耐盐陆地棉品种中9409为材料,从对照和盐胁迫材料的叶片转录组数据检测SNP,并进行GO和Pathway注释。从对照(ck,c)和处理(salt-treated,s)中分别检测到SNPc(对照中的SNP)12 659个、SNPs...为了挖掘陆地棉可能与耐盐性相关的功能SNP,本实验以耐盐陆地棉品种中9409为材料,从对照和盐胁迫材料的叶片转录组数据检测SNP,并进行GO和Pathway注释。从对照(ck,c)和处理(salt-treated,s)中分别检测到SNPc(对照中的SNP)12 659个、SNPs(处理材料的SNP)16 871个,其中对照特有SNP(SNPc-only)2 102个,盐胁迫后样品特有SNP(SNPs-only)4 547个。GO注释分析发现gene^(SNPc),gene^(SNPs),gene^(SNPc-onl)y在分子功能、细胞组分、生物进程富集的比例基本一致,而gene SNPs-only在每个分类中的基因比例都明显大于前三个。Pathway分析表明,gene^(SNPc),gene^(SNPs),gene^(SNPc-only),gene^(SNPs-only)显著富集的通路也不完全相同。以磷酸肌醇代谢途径和茉莉酸信号途径为重点,qRT-PCR(Quantitative Real Time PCR)验证通路中的差异表达基因,并初步分析这些基因上SNP的位置、酶切效应以及是否会导致氨基酸变异。展开更多
In clinical practice, it is challenging to elucidate the location of the lesion in a patient＇s nervous system that is causing the neurologic symptoms, because lesions are often microscopic and cannot be revealed by c...In clinical practice, it is challenging to elucidate the location of the lesion in a patient＇s nervous system that is causing the neurologic symptoms, because lesions are often microscopic and cannot be revealed by conventional evaluation methods.展开更多
文摘为了挖掘陆地棉可能与耐盐性相关的功能SNP,本实验以耐盐陆地棉品种中9409为材料,从对照和盐胁迫材料的叶片转录组数据检测SNP,并进行GO和Pathway注释。从对照(ck,c)和处理(salt-treated,s)中分别检测到SNPc(对照中的SNP)12 659个、SNPs(处理材料的SNP)16 871个,其中对照特有SNP(SNPc-only)2 102个,盐胁迫后样品特有SNP(SNPs-only)4 547个。GO注释分析发现gene^(SNPc),gene^(SNPs),gene^(SNPc-onl)y在分子功能、细胞组分、生物进程富集的比例基本一致,而gene SNPs-only在每个分类中的基因比例都明显大于前三个。Pathway分析表明,gene^(SNPc),gene^(SNPs),gene^(SNPc-only),gene^(SNPs-only)显著富集的通路也不完全相同。以磷酸肌醇代谢途径和茉莉酸信号途径为重点,qRT-PCR(Quantitative Real Time PCR)验证通路中的差异表达基因,并初步分析这些基因上SNP的位置、酶切效应以及是否会导致氨基酸变异。
文摘In clinical practice, it is challenging to elucidate the location of the lesion in a patient＇s nervous system that is causing the neurologic symptoms, because lesions are often microscopic and cannot be revealed by conventional evaluation methods.
