目的采用RNA干扰(RNAi)方法,研究当Delta N p63基因的表达沉默后,对人膀胱癌裸鼠皮下移植模型肿瘤的抑制作用,并检测对细胞周期素依赖性激酶4(CDK4)基因和蛋白表达的影响,进一步揭示Delta N p63基因在膀胱肿瘤中的作用机制。方法首先构...目的采用RNA干扰(RNAi)方法,研究当Delta N p63基因的表达沉默后,对人膀胱癌裸鼠皮下移植模型肿瘤的抑制作用,并检测对细胞周期素依赖性激酶4(CDK4)基因和蛋白表达的影响,进一步揭示Delta N p63基因在膀胱肿瘤中的作用机制。方法首先构建Delta N p63基因特异性短发卡RNA(shRNA)表达质粒,将表达质粒转染至裸鼠皮下移植模型肿瘤瘤体内,比较各组肿瘤体积,光镜下检测肿瘤组织病理学改变,RT-PCR方法检测CKD4基因的表达变化,Western blot和SP免疫组化检测CKD4蛋白表达变化。结果质粒注射到肿瘤8周后,治疗组、对照组与生理盐水组比较,治疗组与生理盐水组瘤体积比较差异有统计学意义(P<0.01),抑瘤率74.62%。光镜结果显示治疗组肿瘤生长受到明显抑制,细胞核分裂象明显减少,可见坏死和凋亡肿瘤细胞、局部炎症细胞浸润。RT-PCR结果显示治疗组CKD4基因表达降低,蛋白检测结果显示CKD4蛋白表达下降。结论沉默Delta N p63表达后,可显著抑制人膀胱癌裸鼠肿瘤模型中肿瘤的生长,Delta N p63可能通过抑制CKD4的表达而抑制肿瘤的细胞生长。展开更多
To provide a computational efficient forward model with moderate accuracy for rapid 3D optical tomography in small volumes,radiative transport in the delta-P1 approximation combined with the approximation of the recip...To provide a computational efficient forward model with moderate accuracy for rapid 3D optical tomography in small volumes,radiative transport in the delta-P1 approximation combined with the approximation of the reciprocity was examined.Perturbations of optical signals caused by absorption and fluorescence heterogeneities submerged in a resin-based liquid phantom with background parameters close to rat brain tissues were measured using a recently constructed laminar optical tomography system.These measured perturbations were used to examine the theoretically calculated fluence perturbations based on the delta-P1 approximation and the reciprocity approximation.Results show that the errors between the predicted and measured data are acceptable,especially for fluorescence perturbations.展开更多
文摘目的采用RNA干扰(RNAi)方法,研究当Delta N p63基因的表达沉默后,对人膀胱癌裸鼠皮下移植模型肿瘤的抑制作用,并检测对细胞周期素依赖性激酶4(CDK4)基因和蛋白表达的影响,进一步揭示Delta N p63基因在膀胱肿瘤中的作用机制。方法首先构建Delta N p63基因特异性短发卡RNA(shRNA)表达质粒,将表达质粒转染至裸鼠皮下移植模型肿瘤瘤体内,比较各组肿瘤体积,光镜下检测肿瘤组织病理学改变,RT-PCR方法检测CKD4基因的表达变化,Western blot和SP免疫组化检测CKD4蛋白表达变化。结果质粒注射到肿瘤8周后,治疗组、对照组与生理盐水组比较,治疗组与生理盐水组瘤体积比较差异有统计学意义(P<0.01),抑瘤率74.62%。光镜结果显示治疗组肿瘤生长受到明显抑制,细胞核分裂象明显减少,可见坏死和凋亡肿瘤细胞、局部炎症细胞浸润。RT-PCR结果显示治疗组CKD4基因表达降低,蛋白检测结果显示CKD4蛋白表达下降。结论沉默Delta N p63表达后,可显著抑制人膀胱癌裸鼠肿瘤模型中肿瘤的生长,Delta N p63可能通过抑制CKD4的表达而抑制肿瘤的细胞生长。
文摘To provide a computational efficient forward model with moderate accuracy for rapid 3D optical tomography in small volumes,radiative transport in the delta-P1 approximation combined with the approximation of the reciprocity was examined.Perturbations of optical signals caused by absorption and fluorescence heterogeneities submerged in a resin-based liquid phantom with background parameters close to rat brain tissues were measured using a recently constructed laminar optical tomography system.These measured perturbations were used to examine the theoretically calculated fluence perturbations based on the delta-P1 approximation and the reciprocity approximation.Results show that the errors between the predicted and measured data are acceptable,especially for fluorescence perturbations.
