目的:分析讨论使用益气散聚方治疗方法对肥胖相关性肾病进行治疗后患者的临床表现和和疗效情况并探索内在的腺苷酸活化蛋白激酶(AMP-Activated Protein Kinase,AMPK)诱发炎症因素在患者体内是如何依据其作用联系进行表达的。方法:纳入...目的:分析讨论使用益气散聚方治疗方法对肥胖相关性肾病进行治疗后患者的临床表现和和疗效情况并探索内在的腺苷酸活化蛋白激酶(AMP-Activated Protein Kinase,AMPK)诱发炎症因素在患者体内是如何依据其作用联系进行表达的。方法:纳入从中医医学角度认为属于脾气亏虚、实邪积聚类型的患者,这些患者具备肥胖相关性肾病,按照分组原则,纳入患病情况和病史相近的60位患者,按照随机抽选分组的原则,将这些患者分为药物治疗组与对照组,每一组均有30位随机抽取的患者。治疗组患者使用益气散聚方进行药物治疗,而对照组,只需给以一定剂量的安慰剂(1/10剂量的益气散聚方)进行基本的治疗,实际治疗过程所需时间为6个月。在治疗过程完成之后,仔细观察两组患者在治疗前后在临床上的病证表现以及尿微量白蛋白指标、尿微量白蛋白/肌酐指标、24 h时间内尿蛋白定量指标、肝肾功能变化情况,而后检查其体内血清中AMPK因子的活性情况以体现体内的AMPK表达情况、白细胞介素-6、C反应蛋白、肿瘤坏死因子-α等多种不同种类的炎症因子的具体表达数据。结果:入组60例,有7位患者因为某些原因脱落,因此只有53位患者的最终数据进入到数据统计与分析阶段,最终的分析结果显示为两个组别在完成相应的治疗疗程之后与治疗前的临床证候积分数据相比,均有不同程度的降低,即病情有不同程度的好转(P<0.01),而尿液中指数,如微量白蛋白指数、微量白蛋白/肌酐指数和标准的24 h时间尿蛋白定量指数均有一定程度下降(P<0.01),AMPK活性升高(P<0.01),C反应蛋白、肿瘤坏死因子-α、白细胞介素-6表达下降(P<0.05);因此可以认为,在治疗组中的患者经过治疗后病情好转程度要显著好于对照组的患者(P<0.05)。结论:适当剂量的益气散聚方能改善脾气亏虚的患者所患有的与肥胖相关性肾病�展开更多
Objective： To investigate the effects and mechanisms of Gong-tone music on the immunological function in rats with the Chinese medicine syndrome of Liver （Gan）-qi stagnation and Spleen （Pi）-qi deficiency （LSSD）...Objective： To investigate the effects and mechanisms of Gong-tone music on the immunological function in rats with the Chinese medicine syndrome of Liver （Gan）-qi stagnation and Spleen （Pi）-qi deficiency （LSSD）. Methods： Twenty five male Wistar rats of SPF grade were randomly divided into 5 groups： normal group, model group, Xiaoyao Powder （逍遥散） group, Gong-tone group and combined group （the combination of Gong-tone and Xiaoyao Powder）, with 5 rats in each group. The rat model for the Chinese medicine syndrome of LSSD was induced by chronic bandage and irregular diet. The course of treatment was 21 days. After the treatment, the levels of serum gastrin and IgG were detected by enzyme-linked immunoabsorbent assay （ELISA）. Phagocytosis of macrophages was detected by the neutral red uptake assay and T cell proliferation was investigated by 3-（4,5-dimethylthiazolyl）-2,5-diphenyltetrazolium bromide （MTT） assay. Results： The serum gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in the model group were significantly decreased compared with those in the normal group （P〈0.05）. Compared with those in the model group, the serum levels of gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in Gong-tone, Xiaoyao Powder, and combined groups were significantly increased （P〈0.05）. The combined group was superior to either Gong-tone group or Xiaoyao Powder group. Conclusion： Gong-tone music may upregulate the immunological function and play a role in adjuvant therapy in the Chinese syndrome of LSSD.展开更多
文摘目的:分析讨论使用益气散聚方治疗方法对肥胖相关性肾病进行治疗后患者的临床表现和和疗效情况并探索内在的腺苷酸活化蛋白激酶(AMP-Activated Protein Kinase,AMPK)诱发炎症因素在患者体内是如何依据其作用联系进行表达的。方法:纳入从中医医学角度认为属于脾气亏虚、实邪积聚类型的患者,这些患者具备肥胖相关性肾病,按照分组原则,纳入患病情况和病史相近的60位患者,按照随机抽选分组的原则,将这些患者分为药物治疗组与对照组,每一组均有30位随机抽取的患者。治疗组患者使用益气散聚方进行药物治疗,而对照组,只需给以一定剂量的安慰剂(1/10剂量的益气散聚方)进行基本的治疗,实际治疗过程所需时间为6个月。在治疗过程完成之后,仔细观察两组患者在治疗前后在临床上的病证表现以及尿微量白蛋白指标、尿微量白蛋白/肌酐指标、24 h时间内尿蛋白定量指标、肝肾功能变化情况,而后检查其体内血清中AMPK因子的活性情况以体现体内的AMPK表达情况、白细胞介素-6、C反应蛋白、肿瘤坏死因子-α等多种不同种类的炎症因子的具体表达数据。结果:入组60例,有7位患者因为某些原因脱落,因此只有53位患者的最终数据进入到数据统计与分析阶段,最终的分析结果显示为两个组别在完成相应的治疗疗程之后与治疗前的临床证候积分数据相比,均有不同程度的降低,即病情有不同程度的好转(P<0.01),而尿液中指数,如微量白蛋白指数、微量白蛋白/肌酐指数和标准的24 h时间尿蛋白定量指数均有一定程度下降(P<0.01),AMPK活性升高(P<0.01),C反应蛋白、肿瘤坏死因子-α、白细胞介素-6表达下降(P<0.05);因此可以认为,在治疗组中的患者经过治疗后病情好转程度要显著好于对照组的患者(P<0.05)。结论:适当剂量的益气散聚方能改善脾气亏虚的患者所患有的与肥胖相关性肾病�
基金Supported by the National Natural Science Foundation of China(No.30701105)Beijing Municipal Educational Fund[(2007) No.20]
文摘Objective： To investigate the effects and mechanisms of Gong-tone music on the immunological function in rats with the Chinese medicine syndrome of Liver （Gan）-qi stagnation and Spleen （Pi）-qi deficiency （LSSD）. Methods： Twenty five male Wistar rats of SPF grade were randomly divided into 5 groups： normal group, model group, Xiaoyao Powder （逍遥散） group, Gong-tone group and combined group （the combination of Gong-tone and Xiaoyao Powder）, with 5 rats in each group. The rat model for the Chinese medicine syndrome of LSSD was induced by chronic bandage and irregular diet. The course of treatment was 21 days. After the treatment, the levels of serum gastrin and IgG were detected by enzyme-linked immunoabsorbent assay （ELISA）. Phagocytosis of macrophages was detected by the neutral red uptake assay and T cell proliferation was investigated by 3-（4,5-dimethylthiazolyl）-2,5-diphenyltetrazolium bromide （MTT） assay. Results： The serum gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in the model group were significantly decreased compared with those in the normal group （P〈0.05）. Compared with those in the model group, the serum levels of gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in Gong-tone, Xiaoyao Powder, and combined groups were significantly increased （P〈0.05）. The combined group was superior to either Gong-tone group or Xiaoyao Powder group. Conclusion： Gong-tone music may upregulate the immunological function and play a role in adjuvant therapy in the Chinese syndrome of LSSD.
