The purpose of this study was to determine the relationship between hypermethylation of DACT1 gene pro-moter and lower mRNA expression in bladder urothelial carcinoma tissue.The methylation status of 29 urothelial car...The purpose of this study was to determine the relationship between hypermethylation of DACT1 gene pro-moter and lower mRNA expression in bladder urothelial carcinoma tissue.The methylation status of 29 urothelial carcinoma samples and 29 normal tissue samples were examined by methylation-specific polymerase chain reac-tion（MSP）.The DACT1 mRNA transcript levels and DACT1 protein levels in all samples were then evaluated to define the relationship between the methylation status of the DACT1 promoter and its expression at the transcrip-tional and translational levels.Decreased expression of DACT1 was detected in 89.66% of urothelial carcinomas（26/29;P 〈 0.005）.Promoter hypermethylation was found in 58.62%（17/29） urothelial carcinomas and 25%（7/29） normal tissues,respectively（P 〈 0.05）.DACT1 expression was lower in tissues where the DACT1 gene promoter was hypermethylated than in unmethylated tissues（0.25±0.17 vs 0.69±0.30,P 〈 0.05）.DACT1 gene hyper-methylation was closely related to tumor size,grade and stage（P 〈 0.05）.Our results indicate that silencing and downregulation of DACT1 mRNA may be implicated in carcinogenesis and the progression of bladder urothelial carcinoma,and may be a potential prognostic factor.展开更多
目的探讨DACT1在结肠癌中的表达及其对结肠癌细胞SW480增殖、迁移和侵袭的影响。方法 Western blot检测DACT1在结肠癌组织中的表达;CCK-8进行细胞增殖实验;细胞划痕实验检测细胞迁移能力;用Transwell小室法检测细胞转染前后侵袭力改变...目的探讨DACT1在结肠癌中的表达及其对结肠癌细胞SW480增殖、迁移和侵袭的影响。方法 Western blot检测DACT1在结肠癌组织中的表达;CCK-8进行细胞增殖实验;细胞划痕实验检测细胞迁移能力;用Transwell小室法检测细胞转染前后侵袭力改变。结果 21例结肠癌组织中有14例DACT1表达增高,DACT1高表达的SW480增殖能力明显增强(P<0.05),细胞划痕后24h,DACT1高表达的细胞划痕宽度恢复70%,而对照组划痕宽度恢复20%(P<0.05)。实验组SW480细胞侵袭数量明显高于对照组(P<0.05)。结论 DACT1在结肠癌中高表达并促进结肠癌细胞的增殖、迁移和侵袭。展开更多
目的:探讨肾透明细胞癌中β-环连蛋白抑制基因1(dapper homolog 1,antagonist of beta-catenin,Dact1)表达与其启动子CpG岛甲基化状态的关系,并探究他们之间的关系及在肾透明细胞癌发生中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)...目的:探讨肾透明细胞癌中β-环连蛋白抑制基因1(dapper homolog 1,antagonist of beta-catenin,Dact1)表达与其启动子CpG岛甲基化状态的关系,并探究他们之间的关系及在肾透明细胞癌发生中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)及甲基化特异性PCR(methylation specific PCR,MSP)分别检测30例癌组织及相应的癌旁对照组织中Dact1基因mRNA的表达和其启动子甲基化状态。结果:癌组织组中,19例Dact1基因mRNA失表达(63%),7例表达低于癌旁对照组(23%);癌旁对照组织组中,2例Dact1基因mRNA低表达(6%)。Dactl基因在癌组织、癌旁对照组织中的甲基化阳性率分别为70%、6%,2组比较差异有统计学意义(P<O.001),Dactl基因mRNA的失表达或低表达与其启动子甲基化状态的相关性分析有统计学意义(P<0.05)。结论:Dact1基因mRNA的失表达或低表达可能参与了肾透明细胞癌的发生发展,其失表达可能与Dactl启动子区异常甲基化相关。展开更多
文摘The purpose of this study was to determine the relationship between hypermethylation of DACT1 gene pro-moter and lower mRNA expression in bladder urothelial carcinoma tissue.The methylation status of 29 urothelial carcinoma samples and 29 normal tissue samples were examined by methylation-specific polymerase chain reac-tion（MSP）.The DACT1 mRNA transcript levels and DACT1 protein levels in all samples were then evaluated to define the relationship between the methylation status of the DACT1 promoter and its expression at the transcrip-tional and translational levels.Decreased expression of DACT1 was detected in 89.66% of urothelial carcinomas（26/29;P 〈 0.005）.Promoter hypermethylation was found in 58.62%（17/29） urothelial carcinomas and 25%（7/29） normal tissues,respectively（P 〈 0.05）.DACT1 expression was lower in tissues where the DACT1 gene promoter was hypermethylated than in unmethylated tissues（0.25±0.17 vs 0.69±0.30,P 〈 0.05）.DACT1 gene hyper-methylation was closely related to tumor size,grade and stage（P 〈 0.05）.Our results indicate that silencing and downregulation of DACT1 mRNA may be implicated in carcinogenesis and the progression of bladder urothelial carcinoma,and may be a potential prognostic factor.
文摘目的探讨DACT1在结肠癌中的表达及其对结肠癌细胞SW480增殖、迁移和侵袭的影响。方法 Western blot检测DACT1在结肠癌组织中的表达;CCK-8进行细胞增殖实验;细胞划痕实验检测细胞迁移能力;用Transwell小室法检测细胞转染前后侵袭力改变。结果 21例结肠癌组织中有14例DACT1表达增高,DACT1高表达的SW480增殖能力明显增强(P<0.05),细胞划痕后24h,DACT1高表达的细胞划痕宽度恢复70%,而对照组划痕宽度恢复20%(P<0.05)。实验组SW480细胞侵袭数量明显高于对照组(P<0.05)。结论 DACT1在结肠癌中高表达并促进结肠癌细胞的增殖、迁移和侵袭。
文摘目的:探讨肾透明细胞癌中β-环连蛋白抑制基因1(dapper homolog 1,antagonist of beta-catenin,Dact1)表达与其启动子CpG岛甲基化状态的关系,并探究他们之间的关系及在肾透明细胞癌发生中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)及甲基化特异性PCR(methylation specific PCR,MSP)分别检测30例癌组织及相应的癌旁对照组织中Dact1基因mRNA的表达和其启动子甲基化状态。结果:癌组织组中,19例Dact1基因mRNA失表达(63%),7例表达低于癌旁对照组(23%);癌旁对照组织组中,2例Dact1基因mRNA低表达(6%)。Dactl基因在癌组织、癌旁对照组织中的甲基化阳性率分别为70%、6%,2组比较差异有统计学意义(P<O.001),Dactl基因mRNA的失表达或低表达与其启动子甲基化状态的相关性分析有统计学意义(P<0.05)。结论:Dact1基因mRNA的失表达或低表达可能参与了肾透明细胞癌的发生发展,其失表达可能与Dactl启动子区异常甲基化相关。
文摘目的:检测贲门腺癌(gastric cardia adenocarcinoma,GCA)及相应癌旁非肿瘤组织中β-环连蛋白抑制基因1(dishevelled-binding antagonist of beta-catenin 1,DACT-1)的甲基化状态,并探讨其临床意义。方法:应用甲基化特异性PCR(methylation specific PCR,MSP)、定量RT-PCR的方法分别检测112例贲门腺癌(河北医科大学第四医院外科和磁县肿瘤医院胸外科于2006-2014年收治)及相应癌旁非肿瘤组织中DACT-1基因的甲基化状态及其mRNA表达情况。结果:在贲门腺癌组织中,DACT-1基因的甲基化率为51.8%(58/112),癌旁非肿瘤组织中该基因的甲基化率为17.6%(20/112),癌组织中DACT-1基因发生甲基化的频率明显高于癌旁非肿瘤组织(P<0.01);癌组织中DACT-1基因mRNA的表达量为0.580±0.143,明显低于癌旁非肿瘤组织(0.654±0.110,P<0.01);在DACT-1基因甲基化的贲门癌组织中该基因mRNA的表达量为0.488±0.097,明显低于该基因未甲基化的贲门癌组织(0.675±0.120),且该基因甲基化状态与其mRNA表达量相关(P<0.01)。癌组织中DACT-1基因的高甲基化状态与肿瘤患者的淋巴结转移情况及上消化道肿瘤家族史有关(P<0.05),而与肿瘤患者的年龄、性别及肿瘤组织的病理分级、临床分期均无关(P>0.05)。结论:贲门腺癌中基因Cp G岛的高甲基化可能是DACT-1基因表达下调的机制之一;DACT-1基因启动子区的甲基化状态有望为贲门腺癌临床辅助诊断和预后评估提供新的指标。
