17 indices are selected,such as the growth rate of total regional output value,the proportion of tertiary industry in GDP,per capita financial expenditure,and soil erosion rate of Guizhou Province in 2009.According to...17 indices are selected,such as the growth rate of total regional output value,the proportion of tertiary industry in GDP,per capita financial expenditure,and soil erosion rate of Guizhou Province in 2009.According to the relevant indices data of statistical yearbook and governmental website,by using the method of factor analysis and the method of cluster analysis,we assess the competitiveness of county economy in 88 counties of Guizhou Province.The results show that the competitiveness of county economy in Guizhou Province is impacted by factors of location and economic foundation.In addition,the resources environment,economic structure,economic developmental speed and other factors also impact the competitiveness of county economy in Guizhou Province.Based on these,in the light of the developmental characteristics of different counties in conjunction with different developmental advantages in different regions,we should adopt different developmental strategies according to local conditions,which is significant to rapid,healthy and sustainable development of county economy in Guizhou Province.展开更多
●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in spe...●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing展开更多
We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was sho...We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.展开更多
以大豆油(33种)、花生油(39种)、葵花籽油(17种)、玉米油(10种)、棕榈油(28种)、芝麻油(37种)和菜籽油(58种)为样品,采用反相高效液相色谱法(RP-HPLC)分别测定其甘三酯组成.以乙腈和二氯甲烷为流动相,蒸发光散射检测器检测,通过JUPITER ...以大豆油(33种)、花生油(39种)、葵花籽油(17种)、玉米油(10种)、棕榈油(28种)、芝麻油(37种)和菜籽油(58种)为样品,采用反相高效液相色谱法(RP-HPLC)分别测定其甘三酯组成.以乙腈和二氯甲烷为流动相,蒸发光散射检测器检测,通过JUPITER C18色谱柱将油脂分离为多簇峰(38个);随机取2/3样品数量作为定标集,使用NIRCal-5.2软件的数据处理向导,以甘三酯相对含量为变量,对其进行log(logarithm)对数处理和ncl(Normalization by Closure)标准化处理,使用聚类分析-主成分分析(CLU-PCA)方法,经过优化识别模型,7种植物纯油被正确识别为7类;其余1/3样品为验证集,除玉米油和菜籽油的识别率为90%和92%外,其他识别模型的准确率和验证准确率均为100%.展开更多
基金Supported by Soft Science United Fund of Technology Department of Guizhou Province([2010]2LKC2015)Special Program of Humanities and Social Sciences of Technology Department of Guizhou Province(09ZX119)
文摘17 indices are selected,such as the growth rate of total regional output value,the proportion of tertiary industry in GDP,per capita financial expenditure,and soil erosion rate of Guizhou Province in 2009.According to the relevant indices data of statistical yearbook and governmental website,by using the method of factor analysis and the method of cluster analysis,we assess the competitiveness of county economy in 88 counties of Guizhou Province.The results show that the competitiveness of county economy in Guizhou Province is impacted by factors of location and economic foundation.In addition,the resources environment,economic structure,economic developmental speed and other factors also impact the competitiveness of county economy in Guizhou Province.Based on these,in the light of the developmental characteristics of different counties in conjunction with different developmental advantages in different regions,we should adopt different developmental strategies according to local conditions,which is significant to rapid,healthy and sustainable development of county economy in Guizhou Province.
基金Supported by Regional Science Foundation Project of the National Natural Science Foundation of China(No.82060827,No.82260891)The Key Discipline of Universities in the“14th Five-Year Plan”Autonomous Region-Traditional Chinese Medicine at Xinjiang Medical University.
文摘●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing
文摘We have studied the expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well as their hypoxic regulation. It was shown that the expression levels of activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase?2, C polypeptide (TGM2), leukemia inhibitory factor (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13) are significantly down-regulated in glioma cells with the knockdown of IRE1 signaling enzyme. It was also shown that in glioma cells subjected to hypoxia, the expression levels of PSAT1, TSPAN13, EIF2AK3, and TGM2 genes were up-regulated, whereas the expression of ATF6 gene was down-regulated. At the same time, the expression levels of LIF, CLU, and ADGRE5 genes did not change in response to hypoxic treatment.?Furthermore, inhibition of IRE1, a key effector of an unfolded protein response pathway, modified the effect of hypoxia on the expression of most studied genes. Present study demonstrates that IRE1 knockdown down-regulated the expression of most studied genes and modified their hypoxic regulation and that these changes possibly contributed to the suppression of glioma growth in cells without IRE1 signaling enzyme function.
文摘以大豆油(33种)、花生油(39种)、葵花籽油(17种)、玉米油(10种)、棕榈油(28种)、芝麻油(37种)和菜籽油(58种)为样品,采用反相高效液相色谱法(RP-HPLC)分别测定其甘三酯组成.以乙腈和二氯甲烷为流动相,蒸发光散射检测器检测,通过JUPITER C18色谱柱将油脂分离为多簇峰(38个);随机取2/3样品数量作为定标集,使用NIRCal-5.2软件的数据处理向导,以甘三酯相对含量为变量,对其进行log(logarithm)对数处理和ncl(Normalization by Closure)标准化处理,使用聚类分析-主成分分析(CLU-PCA)方法,经过优化识别模型,7种植物纯油被正确识别为7类;其余1/3样品为验证集,除玉米油和菜籽油的识别率为90%和92%外,其他识别模型的准确率和验证准确率均为100%.
