BACKGROUND: The fluidity of cell membrane can be affected by various factors. Many experiments have confirmed that the ischemia/reperfusion of organic tissue can increase the contents of free radicals, which lead to h...BACKGROUND: The fluidity of cell membrane can be affected by various factors. Many experiments have confirmed that the ischemia/reperfusion of organic tissue can increase the contents of free radicals, which lead to high rigidity and low fluidity of cell membrane, and the conditions can be changed by Chuanxiongqin. OBJECTIVE: To observe the effect and mechanism of Chuanxiongqin hydrochloride on the fluidity of brain cell membrane in rat models of ischemia/reperfusion. DESIGN: A completely randomized controlled animal trial. SETTINGS: Institute of Brain Sciences; Department of Physiology, Medical College, Datong University. MATERIALS: Twenty male grade Ⅰ Wistar rats of 170-220 g were randomly divided into model group (n =10) and control group (n =10). Chuanxiongqin hydrochloride (molecular mass was 172.2) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (batch number: 0817-9803); Spin labelers: 5-doxyl-stearlic acid methylester (5DS), 16-doxyl-stearlic acid methylester (16DS), xanthine, xanthine oxidase (XOD) and 5,5-dimeth-1-pyrroline- N-oxide (DMPO) from Sigma Company; Bruker ESP 300 electron paramagnetic resonance (EPR) spectrometer by Bruker Company (Germany). METHODS: The experiments were carried out in the State Key Laboratory of Natural and Biomimetic Drugs, Peking University from June 2001 to July 2002. In the model group, rats were made into models of cerebral ischemia by 30-minute ligation and 2-hour reperfusion of common carotid arteries; The rats in the control group were not made into models. The order parameter (S) and rotational correlation time (τc) were detected with the ESR spectrometer by means of spin labeling. The greater the S and τc, the smaller the fluidity. Meanwhile, the clearance rate of free radicals was detected with ESR spin trapping. The measurement data were compared using the t test. MAIN OUTCOME MEASURES: The S, τc and clearance rates of O2 · and OH· free radicals were compared between the model group and control group. RES展开更多
Objective:To study the characteristics and rules of medication regimen for patients with Liver insufficiency treated with Danshen Chuanxiongqin Injection.Methods:The inpatient information of Danshen Chuanxiongqin Inje...Objective:To study the characteristics and rules of medication regimen for patients with Liver insufficiency treated with Danshen Chuanxiongqin Injection.Methods:The inpatient information of Danshen Chuanxiongqin Injection with Liver insufficiency was extracted from the hospital information system(HIS)database of 12 Class A tertiary hospitals in China.Tabu search(TS)algorithm was used for obtaining sub-network and density matrix,matrix method or sociogram was used to show the relationship between different sub-networks,searching for core prescriptions of treatment options,associations and strong correlations between multiple drugs.Results:The treatment plan of integrated Chinese and Western medicine of Danshen Chuanxiongqin Injection on Liver insufficiency mainly includes of cerebrovascular disease in acute stress state:Danshen Chuanxiongqin Injection combined with alanyl glutamine;Treatment of acute ischemic cardiovascular and cerebrovascular diseases:Danshen Chuanxiongqin Injection combined with Levocarnitine+acetylsalicylic acid+inosine tablets+brain protein hydrolysate+glutathione+furosemide+Magnesium aspartate+metoprolol;Treatment of ischemic cardiomyopathy angina pectoris with traditional Chinese medicine:Danshen Chuanxiongqin Injection combined with Shexiang Baoxin Pills+Suxiao Jiuxin Pills+Fufang Danshen Drops;Treatment of ischemic cardiovascular disease complicated with Liver insufficiency with traditional Chinese medicine:Danshen Chuanxiongqin Injection combined with Dazhu Hongjingtian Capsule+Shuganning Injection.Conclusion:For Danshen Chuanxiongqin Injection in patients with Liver insufficiency,on the basis of conventional symptomatic treatment,attention should be paid to the liver function of the patients,integrated therapy can be more selective,can improve the efficacy in different degrees.Danshen Chuanxiongqin Injection combination scheme of Liver insufficiency in routine clinical diagnosis and treatment provides a more effective reference for clinical individualized treatment.展开更多
Objective:To study the effects of Danshen Chuanxiongqin therapy on TXA2/PGI2, bone metabolism and stress levels after proximal femoral fracture surgery.Methods:A total of 80 patients with proximal femoral fractures wh...Objective:To study the effects of Danshen Chuanxiongqin therapy on TXA2/PGI2, bone metabolism and stress levels after proximal femoral fracture surgery.Methods:A total of 80 patients with proximal femoral fractures who received open reduction and internal fixation treatment in the First Affiliated Hospital of the Fourth Military Medical University between January 2015 and December 2016 were selected as the research subjects and randomly divided into the experimental group who received postoperative adjuvant Danshen Chuanxiongqin therapy and the control group who received postoperative conventional therapy. The levels of TXA2/PGI2 balance-related indicators, bone metabolism indicators and stress hormones in serum as well as the expression levels of stress molecules in peripheral blood were measured before surgery and 3 d after surgery.Results:Compared with those of same group before surgery, serum 6-keto-PGF1α, t-PA, PICP, BALP and OCN levels of both groups of patients were significantly lower whereas serum TXB2, PAI-1, D-D,β-CTX, RANKL, NE, COR and ANG-Ⅱ levels as well as peripheral blood NF-κB and HSP70 expression were significantly higher after surgery, and serum 6-keto-PGF1α, t-PA, PICP, BALP and OCN levels of experimental group after surgery were higher than those of control group whereas serum TXB2, PAI-1, D-D,β-CTX, RANKL, NE, COR and ANG-Ⅱ levels as well as peripheral blood NF-κB and HSP70 expression were lower than those of control group.Conclusion:Danshen Chuanxiongqin therapy after proximal femoral fracture surgery can adjust the TXA2/PGI2 balance, improve the bone metabolism and reduce the stress level.展开更多
目的观察丹参川芎嗪注射液联合长春西汀治疗急性脑梗死的临床疗效,为临床合理联合用药提供参考。方法选取十堰市太和医院2012年11月—2014年10月收治的急性脑梗死患者326例,随机分为对照组和治疗组,各163例。两组患者均给予常规治疗,对...目的观察丹参川芎嗪注射液联合长春西汀治疗急性脑梗死的临床疗效,为临床合理联合用药提供参考。方法选取十堰市太和医院2012年11月—2014年10月收治的急性脑梗死患者326例,随机分为对照组和治疗组,各163例。两组患者均给予常规治疗,对照组在常规治疗的基础上静滴长春西汀注射液30 mg,以500 m L 0.9%生理盐水稀释,1次/d;治疗组在对照组的基础上静滴丹参川芎嗪注射液40 mg,以500 m L 0.9%生理盐水稀释,1次/d,14 d为1个疗程,两组均治疗1个疗程。计算治疗前后患者日常生活能力(ADL)及欧洲卒中量表(ESS)评分,观察两组的临床疗效,并记录两组治疗前及治疗1、5、9、14 d的脑电图棘波波幅(EEG)和局部脑血流(r CBF),并测定两组治疗前后的血液流变学指标。结果经14 d治疗后,治疗组的ESS评分降低、ADL提高,总有效率达92%,与对照组比较差异有统计学意义(P<0.05);两组的EEG及r CBF均逐步恢复和增高,同组治疗前后差异有统计学意义(P<0.05),且治疗组的EEG及r CBF改善程度均优于对照组,两组比较差异有统计学意义(P<0.05);两组血液流变学指标在治疗后有不同程度的降低,同组治疗前后比较差异均有统计学意义(P<0.05),治疗组的总胆固醇、低密度脂蛋白胆固醇、纤维蛋白、全血高切黏度、全血低切黏度、血小板集聚率等血液流变学指标降低更加明显,与对照组比较差异有统计学意义(P<0.05)。结论丹参川芎嗪注射液联合长春西汀治疗急性脑梗死能显著增加患者r CBF、缩短EEG恢复至正常水平的时间,明显降低ESS评分,改善患者血液流变学效应,对促进患者神经功能恢复及提高生活质量有确切的临床疗效,有一定的临床推广价值。展开更多
文摘BACKGROUND: The fluidity of cell membrane can be affected by various factors. Many experiments have confirmed that the ischemia/reperfusion of organic tissue can increase the contents of free radicals, which lead to high rigidity and low fluidity of cell membrane, and the conditions can be changed by Chuanxiongqin. OBJECTIVE: To observe the effect and mechanism of Chuanxiongqin hydrochloride on the fluidity of brain cell membrane in rat models of ischemia/reperfusion. DESIGN: A completely randomized controlled animal trial. SETTINGS: Institute of Brain Sciences; Department of Physiology, Medical College, Datong University. MATERIALS: Twenty male grade Ⅰ Wistar rats of 170-220 g were randomly divided into model group (n =10) and control group (n =10). Chuanxiongqin hydrochloride (molecular mass was 172.2) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (batch number: 0817-9803); Spin labelers: 5-doxyl-stearlic acid methylester (5DS), 16-doxyl-stearlic acid methylester (16DS), xanthine, xanthine oxidase (XOD) and 5,5-dimeth-1-pyrroline- N-oxide (DMPO) from Sigma Company; Bruker ESP 300 electron paramagnetic resonance (EPR) spectrometer by Bruker Company (Germany). METHODS: The experiments were carried out in the State Key Laboratory of Natural and Biomimetic Drugs, Peking University from June 2001 to July 2002. In the model group, rats were made into models of cerebral ischemia by 30-minute ligation and 2-hour reperfusion of common carotid arteries; The rats in the control group were not made into models. The order parameter (S) and rotational correlation time (τc) were detected with the ESR spectrometer by means of spin labeling. The greater the S and τc, the smaller the fluidity. Meanwhile, the clearance rate of free radicals was detected with ESR spin trapping. The measurement data were compared using the t test. MAIN OUTCOME MEASURES: The S, τc and clearance rates of O2 · and OH· free radicals were compared between the model group and control group. RES
基金Science and Technology Innovation Project of Chinese Academy of Traditional Chinese Medicine(Project No.CI2021A00702)National Key R&D Program"Evidence-based Evaluation of Ten Major Varieties of Chinese Medicines and Classical Formulas for the Treatment of Major Diseases and Demonstration of Their Effect Mechanisms after Marketing"(2018YFC1707400)。
文摘Objective:To study the characteristics and rules of medication regimen for patients with Liver insufficiency treated with Danshen Chuanxiongqin Injection.Methods:The inpatient information of Danshen Chuanxiongqin Injection with Liver insufficiency was extracted from the hospital information system(HIS)database of 12 Class A tertiary hospitals in China.Tabu search(TS)algorithm was used for obtaining sub-network and density matrix,matrix method or sociogram was used to show the relationship between different sub-networks,searching for core prescriptions of treatment options,associations and strong correlations between multiple drugs.Results:The treatment plan of integrated Chinese and Western medicine of Danshen Chuanxiongqin Injection on Liver insufficiency mainly includes of cerebrovascular disease in acute stress state:Danshen Chuanxiongqin Injection combined with alanyl glutamine;Treatment of acute ischemic cardiovascular and cerebrovascular diseases:Danshen Chuanxiongqin Injection combined with Levocarnitine+acetylsalicylic acid+inosine tablets+brain protein hydrolysate+glutathione+furosemide+Magnesium aspartate+metoprolol;Treatment of ischemic cardiomyopathy angina pectoris with traditional Chinese medicine:Danshen Chuanxiongqin Injection combined with Shexiang Baoxin Pills+Suxiao Jiuxin Pills+Fufang Danshen Drops;Treatment of ischemic cardiovascular disease complicated with Liver insufficiency with traditional Chinese medicine:Danshen Chuanxiongqin Injection combined with Dazhu Hongjingtian Capsule+Shuganning Injection.Conclusion:For Danshen Chuanxiongqin Injection in patients with Liver insufficiency,on the basis of conventional symptomatic treatment,attention should be paid to the liver function of the patients,integrated therapy can be more selective,can improve the efficacy in different degrees.Danshen Chuanxiongqin Injection combination scheme of Liver insufficiency in routine clinical diagnosis and treatment provides a more effective reference for clinical individualized treatment.
文摘Objective:To study the effects of Danshen Chuanxiongqin therapy on TXA2/PGI2, bone metabolism and stress levels after proximal femoral fracture surgery.Methods:A total of 80 patients with proximal femoral fractures who received open reduction and internal fixation treatment in the First Affiliated Hospital of the Fourth Military Medical University between January 2015 and December 2016 were selected as the research subjects and randomly divided into the experimental group who received postoperative adjuvant Danshen Chuanxiongqin therapy and the control group who received postoperative conventional therapy. The levels of TXA2/PGI2 balance-related indicators, bone metabolism indicators and stress hormones in serum as well as the expression levels of stress molecules in peripheral blood were measured before surgery and 3 d after surgery.Results:Compared with those of same group before surgery, serum 6-keto-PGF1α, t-PA, PICP, BALP and OCN levels of both groups of patients were significantly lower whereas serum TXB2, PAI-1, D-D,β-CTX, RANKL, NE, COR and ANG-Ⅱ levels as well as peripheral blood NF-κB and HSP70 expression were significantly higher after surgery, and serum 6-keto-PGF1α, t-PA, PICP, BALP and OCN levels of experimental group after surgery were higher than those of control group whereas serum TXB2, PAI-1, D-D,β-CTX, RANKL, NE, COR and ANG-Ⅱ levels as well as peripheral blood NF-κB and HSP70 expression were lower than those of control group.Conclusion:Danshen Chuanxiongqin therapy after proximal femoral fracture surgery can adjust the TXA2/PGI2 balance, improve the bone metabolism and reduce the stress level.
文摘目的观察丹参川芎嗪注射液联合长春西汀治疗急性脑梗死的临床疗效,为临床合理联合用药提供参考。方法选取十堰市太和医院2012年11月—2014年10月收治的急性脑梗死患者326例,随机分为对照组和治疗组,各163例。两组患者均给予常规治疗,对照组在常规治疗的基础上静滴长春西汀注射液30 mg,以500 m L 0.9%生理盐水稀释,1次/d;治疗组在对照组的基础上静滴丹参川芎嗪注射液40 mg,以500 m L 0.9%生理盐水稀释,1次/d,14 d为1个疗程,两组均治疗1个疗程。计算治疗前后患者日常生活能力(ADL)及欧洲卒中量表(ESS)评分,观察两组的临床疗效,并记录两组治疗前及治疗1、5、9、14 d的脑电图棘波波幅(EEG)和局部脑血流(r CBF),并测定两组治疗前后的血液流变学指标。结果经14 d治疗后,治疗组的ESS评分降低、ADL提高,总有效率达92%,与对照组比较差异有统计学意义(P<0.05);两组的EEG及r CBF均逐步恢复和增高,同组治疗前后差异有统计学意义(P<0.05),且治疗组的EEG及r CBF改善程度均优于对照组,两组比较差异有统计学意义(P<0.05);两组血液流变学指标在治疗后有不同程度的降低,同组治疗前后比较差异均有统计学意义(P<0.05),治疗组的总胆固醇、低密度脂蛋白胆固醇、纤维蛋白、全血高切黏度、全血低切黏度、血小板集聚率等血液流变学指标降低更加明显,与对照组比较差异有统计学意义(P<0.05)。结论丹参川芎嗪注射液联合长春西汀治疗急性脑梗死能显著增加患者r CBF、缩短EEG恢复至正常水平的时间,明显降低ESS评分,改善患者血液流变学效应,对促进患者神经功能恢复及提高生活质量有确切的临床疗效,有一定的临床推广价值。
