Soil animal communities of Pinus koraiensis broad_leaved mixed forest were studied.The purpose was to discuss the dynamic characteristic of soil animals and relationship with environmental factors.The ecosystem geogra...Soil animal communities of Pinus koraiensis broad_leaved mixed forest were studied.The purpose was to discuss the dynamic characteristic of soil animals and relationship with environmental factors.The ecosystem geography distribution law of soil animals, soil animals’role and function in ecological system were revealed.In June,August,October each year,three plots were selected. In each plot, four layers were sampled(litter layer,0~10 cm,10~20 cm & 20~30 cm),adding up to 72 soil sample.The area for large_sized soil animals was 50 cm×50 cm,and the area for middle_small_sized soil animals was 10 cm × 10 cm.Separated soil animals through adopting hand_picking method and Tullgren method respectively.Sampled the soil animals in two continuing years.Shannon_Wiener index was adopted to analyze the diversity of soil animals.Monad liner regression was used to search the relationship between soil animals and environmental factors.The dominant groups were Oribatida, Isotomidae.There were relatively great changes in groups and individual numbers of dominant groups and rare groups when the reason changed.The individual numbers of dominant groups and the groups of rare groups changed.The number of individual and group was the most in August.Obviously it correlated with climatic factors in middle temperate zone.The evenness was low and dominance was high. The diversity index was not most.Among the annual fluctuating there was a increasing trend. The evenness was low and dominance was high.The diversity index was low.It conformed with the law of reason change.The correlation between soil animals and atmospheric temperature,rainfall,ground temperature and sunlight showed the correlation with rainfall and soil temperature was the most significant.展开更多
Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfe...Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfected laboratory bred MF (BM F) to albinao mice (AM) was performed to observe the acquired protection Sodiu m dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and enzymelin ked immunoblotting (ELIB) methods were used to recognize 4 different lifecycle stage antigens of S japonicum by sera from wild MF (WMF), BMF, BMFi3 and BMFi9 Tests were also performed on in vitro killing effect of sera and/or lymphocytes from BMF an d WMF to schistosomulae; quantitative determination of C3 and C4 by immuno turb idometry, and interleukin4 (IL4) and antibodies to the 4 stage antigens in s era from WMF, BMF and infected BMF by ELISA Results Compared with the control group, stool eggs per gram (EPG) of A M in the test group was significantly reduced by 8154%, miracidium hatching rate, by 5 0 67%, liver egg counts, by 7207%, the diameter of hepatic egg granuloma, by 7 0 39?m Western blotting probed with the 4 MF sera all revealed 7 specific ban d s for SSA, 3 for AWA and SEA, but none for CA antigens The sera and/or lymphoc yte s from WMF and BMF gave obvious killing effects on schistosomulae with an adjust ed death rate of 6412%-7883% The levels ofnatural antibodiesproduced b y MF to S japonicum were in the following order: antiSSA>antiAWA>antiS EA>an tiCA, all of which increased significantly after the infection Serum levels o f C3, C4 and IL4 in uninfected BMF were significantly higher than those in AM After infection, levels of C3 and C4 were further increased respective ly by 7283% and 29549% in the 4th week and IL4 by 30383% in the 9th day Conclusions Immunological characteristics of innate resistance in M f o rtis to S japonicum infection were existed with no significant difference betw een WMF and BMF展开更多
文摘Soil animal communities of Pinus koraiensis broad_leaved mixed forest were studied.The purpose was to discuss the dynamic characteristic of soil animals and relationship with environmental factors.The ecosystem geography distribution law of soil animals, soil animals’role and function in ecological system were revealed.In June,August,October each year,three plots were selected. In each plot, four layers were sampled(litter layer,0~10 cm,10~20 cm & 20~30 cm),adding up to 72 soil sample.The area for large_sized soil animals was 50 cm×50 cm,and the area for middle_small_sized soil animals was 10 cm × 10 cm.Separated soil animals through adopting hand_picking method and Tullgren method respectively.Sampled the soil animals in two continuing years.Shannon_Wiener index was adopted to analyze the diversity of soil animals.Monad liner regression was used to search the relationship between soil animals and environmental factors.The dominant groups were Oribatida, Isotomidae.There were relatively great changes in groups and individual numbers of dominant groups and rare groups when the reason changed.The individual numbers of dominant groups and the groups of rare groups changed.The number of individual and group was the most in August.Obviously it correlated with climatic factors in middle temperate zone.The evenness was low and dominance was high. The diversity index was not most.Among the annual fluctuating there was a increasing trend. The evenness was low and dominance was high.The diversity index was low.It conformed with the law of reason change.The correlation between soil animals and atmospheric temperature,rainfall,ground temperature and sunlight showed the correlation with rainfall and soil temperature was the most significant.
文摘Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfected laboratory bred MF (BM F) to albinao mice (AM) was performed to observe the acquired protection Sodiu m dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and enzymelin ked immunoblotting (ELIB) methods were used to recognize 4 different lifecycle stage antigens of S japonicum by sera from wild MF (WMF), BMF, BMFi3 and BMFi9 Tests were also performed on in vitro killing effect of sera and/or lymphocytes from BMF an d WMF to schistosomulae; quantitative determination of C3 and C4 by immuno turb idometry, and interleukin4 (IL4) and antibodies to the 4 stage antigens in s era from WMF, BMF and infected BMF by ELISA Results Compared with the control group, stool eggs per gram (EPG) of A M in the test group was significantly reduced by 8154%, miracidium hatching rate, by 5 0 67%, liver egg counts, by 7207%, the diameter of hepatic egg granuloma, by 7 0 39?m Western blotting probed with the 4 MF sera all revealed 7 specific ban d s for SSA, 3 for AWA and SEA, but none for CA antigens The sera and/or lymphoc yte s from WMF and BMF gave obvious killing effects on schistosomulae with an adjust ed death rate of 6412%-7883% The levels ofnatural antibodiesproduced b y MF to S japonicum were in the following order: antiSSA>antiAWA>antiS EA>an tiCA, all of which increased significantly after the infection Serum levels o f C3, C4 and IL4 in uninfected BMF were significantly higher than those in AM After infection, levels of C3 and C4 were further increased respective ly by 7283% and 29549% in the 4th week and IL4 by 30383% in the 9th day Conclusions Immunological characteristics of innate resistance in M f o rtis to S japonicum infection were existed with no significant difference betw een WMF and BMF