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Towards a global One Health index: a potential assessment tool for One Health performance 被引量:12
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作者 Xiao-Xi Zhang Jing-Shu Liu +35 位作者 Le-Fei Han Shang Xia Shi-Zhu Li Odel YLi Kokouvi Kassegne Min Li Kun Yin Qin-Qin Hu Le-Shan Xiu Yong-Zhang Zhu Liang-Yu Huang Xiang-Cheng Wang Yi Zhang Han-Qing Zhao Jing-Xian Yin Tian-Ge Jiang Qin Li Si-Wei Fei Si-Yu Gu Fu-Min Chen Nan Zhou Zi-Le Cheng Yi Xie Hui-Min Li Jin Chen Zhao-Yu Guo Jia-Xin Feng Lin Ai Jing-Bo Xue Qian Ye Liz Grant Jun-Xia Song Geoff Simm Jürg Utzinger Xiao-Kui Guo Xiao-Nong Zhou 《Infectious Diseases of Poverty》 SCIE 2022年第3期12-26,共15页
Background:A One Health approach has been increasingly mainstreamed by the international community, as it provides for holistic thinking in recognizing the close links and inter-dependence of the health of humans, ani... Background:A One Health approach has been increasingly mainstreamed by the international community, as it provides for holistic thinking in recognizing the close links and inter-dependence of the health of humans, animals and the environment. However, the dearth of real-world evidence has hampered application of a One Health approach in shaping policies and practice. This study proposes the development of a potential evaluation tool for One Health performance, in order to contribute to the scientific measurement of One Health approach and the identification of gaps where One Health capacity building is most urgently needed.Methods:We describe five steps towards a global One Health index (GOHI), including (i) framework formulation;(ii) indicator selection;(iii) database building;(iv) weight determination;and (v) GOHI scores calculation. A cell-like framework for GOHI is proposed, which comprises an external drivers index (EDI), an intrinsic drivers index (IDI) and a core drivers index (CDI). We construct the indicator scheme for GOHI based on this framework after multiple rounds of panel discussions with our expert advisory committee. A fuzzy analytical hierarchy process is adopted to determine the weights for each of the indicators.Results:The weighted indicator scheme of GOHI comprises three first-level indicators, 13 second-level indicators, and 57 third-level indicators. According to the pilot analysis based on the data from more than 200 countries/territories the GOHI scores overall are far from ideal (the highest score of 65.0 out of a maximum score of 100), and we found considerable variations among different countries/territories (31.8–65.0). The results from the pilot analysis are consistent with the results from a literature review, which suggests that a GOHI as a potential tool for the assessment of One Health performance might be feasible.Conclusions:GOHI—subject to rigorous validation—would represent the world’s first evaluation tool that constructs the conceptual framework from a holistic per 展开更多
关键词 Antimicrobial resistance cell-like framework Climate change Food security Global One Health index(GOHI) Global performance assessment GOVERNANCE Zoonotic diseases
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Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats 被引量:6
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作者 Taotao Hu Shusen Chang +8 位作者 Fang Qi Zhonghui Zhang Jiayin Chen Lingli Jiang Dali Wang Chengliang Deng Kaiyu Nie Guangchao Xu Zairong Wei 《Burns & Trauma》 SCIE 2023年第1期375-392,共18页
Background:Schwann cell-like cells(SCLCs),differentiated from mesenchymal stem cells,have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical studies.However,certain clinical obstacle... Background:Schwann cell-like cells(SCLCs),differentiated from mesenchymal stem cells,have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical studies.However,certain clinical obstacles limit their application.Hence,the primary aim of this study was to investigate the role of exosomes derived from SCLCs(SCLCs-exo)in peripheral nerve regeneration.Methods:SCLCs were differentiated from human amniotic mesenchymal stem cells(hAMSCs)in vitro and validated by immunofluorescence,real-time quantitative PCR and western blot analysis.Exosomes derived from hAMSCs(hAMSCs-exo)and SCLCs were isolated by ultracentrifugation and validated by nanoparticle tracking analysis,WB analysis and electron microscopy.A prefab-ricated nerve graft was used to deliver hAMSCs-exo or SCLCs-exo in an injured sciatic nerve rat model.The effects of hAMSCs-exo or SCLCs-exo on rat peripheral nerve injury(PNI)regeneration were determined based on the recovery of neurological function and histomorphometric variation.The effects of hAMSCs-exo or SCLCs-exo on Schwann cells were also determined via cell prolifer-ation and migration assessment.Results:SCLCs significantly expressed the Schwann cell markers glial fibrillary acidic protein and S100.Compared to hAMSCs-exo,SCLCs-exo significantly enhanced motor function recov-ery,attenuated gastrocnemius muscle atrophy and facilitated axonal regrowth,myelin forma-tion and angiogenesis in the rat model.Furthermore,hAMSCs-exo and SCLCs-exo were effi-ciently absorbed by Schwann cells.However,compared to hAMSCs-exo,SCLCs-exo signifi-cantly promoted the proliferation and migration of Schwann cells.SCLCs-exo also significantly upregulated the expression of a glial cell-derived neurotrophic factor,myelin positive regulators(SRY-box transcription factor 10,early growth response protein 2 and organic cation/carnitine transporter 6)and myelin proteins(myelin basic protein and myelin protein zero)in Schwann cells.Conclusions:These findings suggest that SCLCs-exo can more efficiently prom 展开更多
关键词 Schwann cell-like cells EXOSOMES Peripheral nerve injury Mesenchymal stem cells Nerve regeneration
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PHF20 collaborates with PARP1 to promote stemness and aggressiveness of neuroblastoma cells through activation of SOX2 and OCT4 被引量:6
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作者 Wenyong Long Wei Zhao +8 位作者 Bo Ning Jing Huang Junjun Chu Linfeng Li Qianquan Ma Changsheng Xing Helen Y. Wang Qing Liu Rong-Fu Wang 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2018年第2期147-160,共14页
The differentiation status of neuroblastoma (NB) strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of sternness and differentiation remain poorly understood. Here,... The differentiation status of neuroblastoma (NB) strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of sternness and differentiation remain poorly understood. Here, we show that plant homeodomain finger-containing protein 20 (PHF20) functions as a critical epigenetic regulator in sustaining stem cell-like phenotype of NB by using CRISPR/Casg-based targeted knockout (KO) for high-throughput screening of gene function in NB cell differentiation. The expression of PHF20 in NB was significantly associated with high aggressiveness of the tumor and poor outcomes for NB patients. Deletion of PHF20 inhibited NB cell proliferation, invasive migration, and stem ceU-Uke traits. Mechanistically, PHF20 interacts with poly(ADP-ribose) polymerase 1 (PARP1) and directly binds to promoter regions of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) to modulate a histone mark associated with active transcription, trimethylation of lysine 4 on histone H3 protein subunit (H3K4me3). Overexpression of OCT4 and SOX2 restored growth and progression of PHF20 KO tumor cells. Consistently, OCT4 and SOX2 protein levels in clinical NB specimens were positively correlated with PHF20 expression. Our results establish PHF20 as a key driver of NB stem cell-like properties and aggressive behaviors, with implications for prognosis and therapy. 展开更多
关键词 PHF20 NEUROBLASTOMA PARP1 cancer stem cell-like traits epigenetic regulation
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Deposition of bio-mimicking graphene sheets with lotus leaf-like and cell-like structures on the nickel substrate 被引量:2
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作者 YANG Juan YAN XlngBin WANG Ying LUO BaoMin WANG LiPing XUE QunJi 《Chinese Science Bulletin》 SCIE EI CAS 2012年第23期3036-3039,共4页
Bio-mimicking graphene films,deposited on textured nickel substrates,were synthesized by the following method:replicating the surface textures of the lotus leaf by polymer duplication,fabricating textured nickel subst... Bio-mimicking graphene films,deposited on textured nickel substrates,were synthesized by the following method:replicating the surface textures of the lotus leaf by polymer duplication,fabricating textured nickel substrates by electroplating on the polymer coated with a Au film,preparing bio-mimicking graphene oxide films on the nickel substrates by vacuum filtration,and electrochemical reduction.By controlling the vacuum filtration,this replica method can not only replicate the lotus leaf structure by a graphene film,but also can achieve a novel cell-like graphene film. 展开更多
关键词 bio-mimicking graphene film lotus leaf-like STRUCTURE cell-like STRUCTURE
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Phenotype-Directed DNA Nanomachine for in Situ Analysis of Stem Cell-like Subpopulation in Breast Cancer
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作者 Ya Cao Huiru Mao +4 位作者 Lingjun Sha Qi Liu Bing Han Jing Zhao Genxi Li 《CCS Chemistry》 CSCD 2024年第4期1034-1046,共13页
The cancer stem cell hypothesis provides a basis for prediction of the recurrence and risk of metastasis in breast cancer.However,the unique expression pattern of stemness markers and the presence of nonstem-like canc... The cancer stem cell hypothesis provides a basis for prediction of the recurrence and risk of metastasis in breast cancer.However,the unique expression pattern of stemness markers and the presence of nonstem-like cancer cells with varied phenotypes have brought great challenges to the characterization of breast cancer stem cells.To address these challenges,a phenotype-directed DNA nanomachine has been designed for high-accuracy labeling and in situ analysis of the stem cell-like subpopulation in breast cancer.The key for the design is to use cell surfaceanchored inputs to activate the nanomachine,which undergoes different branch migration pathways such that the signal strand can only be brought onto the cancer cells having the stem cell-like phenotype.Highly sensitive determination and single-step isolation of the stem cell-like subpopulation were achieved by incorporating functional groups into the signal strand such that the nanomachine was successfully applied in a tumor-bearing mouse model.Overall,the approach provides for a substantial improvement in capability for the analysis of the breast cancer stem cell-like subpopulation,and it is expected that the new approach will advance the use of DNA nanomachines in cancer-related studies. 展开更多
关键词 breast cancer DNA nanomachine stem cell-like subpopulation electrochemical determination single-step isolation phenotypic analysis
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Self-assembly with varying hydrophobic centers:Synthesis of red blood cell-like basic magnesium carbonate microspheres 被引量:2
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作者 Xinlong Ma Guoqing Ning +3 位作者 Bing Chen Chuanlei Qi Xingying Lan Jinsen Gao 《Particuology》 SCIE EI CAS CSCD 2015年第5期145-150,共6页
Basic magnesium carbonate microspheres with a red blood cell (RBC)-like appearance and diameters of ~3μm were synthesized by amphiphilic molecule-participated self-assembly under hydrothermal conditions, In the sel... Basic magnesium carbonate microspheres with a red blood cell (RBC)-like appearance and diameters of ~3μm were synthesized by amphiphilic molecule-participated self-assembly under hydrothermal conditions, In the self-assembly, sodium dodecyl benzene sulfonate served as a template for the formation of Mg(OH)2 spherical micelles and also as a reactant precursor that releases CO2 to react with Mg(OH)2. The growth of the microspheres is driven by the continuous generation of new hydrophobic centers because of the consumption of hydrophilic poles (--SO3-). The surfactant-directed self-assembly can be applied to the synthesis of other carbonate or metallic oxide self-assemblies, indicating that it is a universal self-assembly method for amphiphilic molecules. 展开更多
关键词 SELF-ASSEMBLY Red blood cell-like Basic magnesium carbonate MICROSPHERES
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S100 protein expression during induced Schwann cell-like cell differentiation of rat bone marrow mesenchymal cells in vitro 被引量:1
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作者 Wenting Li Zenglu Xu +1 位作者 Fei Ding Xiaosong Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第3期178-184,共7页
BACKGROUND: S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells (MSCs) that can secrete S100 may provide a beneficial microenvironment for neural... BACKGROUND: S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells (MSCs) that can secrete S100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE: To explore the changes in S100 expression during rat MSCs differentiation into Schwann ceils in vitro. DESIGN, TIME AND SETTING: This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALS: The rabbit anti-S100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat S100 monoclonal antibody was purchased from Sigma, USA. METHODS: MSCs were cultured from adult Sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was measured by flow cytometry. MSCs were induced to differentiate into SC cells. SC cells were stained for S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. S100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURES: S100 protein and mRNA expression. RESULTS: MSCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MSCs were at the G0/G1 phase of the cell cycle. After induction, MSCs displayed morphology changes similar to Schwann cells. Moreover, induction increased S100 mRNA levels. Immunofluorescence showed that MSCs expressed S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased S100 protein levels compared with untreated MSCs. CONCLUSION: MSCs are capable of differentiating into Schwann cells-like cells under conditional induction in vitro, with increasing S100 mRNA and protein expression. 展开更多
关键词 bone marrow mesenchymal stem cells INDUCTION Schwann cell-like cells S100 protein in vitro stem cells neural regeneration
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Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells 被引量:1
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作者 LI WenTing1,SUN HuaLin2,XU ZengLu1,DING Fei2 & GU XiaoSong2 1 Department of Anatomy,Histology and Embryology,Peking Union Medical College,Institute of Basic Medical Science,Chinese Academy of Medical Sciences,Beijing 100005,China 2 Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China 《Science China(Life Sciences)》 SCIE CAS 2009年第3期267-277,共11页
During the last decade,increasing evidence suggested that bone marrow stromal cells(MSCs) have the potential to differentiate into neural lineages.Many studies have reported that MSCs showed morphological changes and ... During the last decade,increasing evidence suggested that bone marrow stromal cells(MSCs) have the potential to differentiate into neural lineages.Many studies have reported that MSCs showed morphological changes and expressed a limited number of neural proteins under experimental conditions.However,no proteomic studies on MSCs differentiated into Schwann cell-like cells have been reported.In this study,we isolated MSCs from adult Sprague-Dawley rat femur and tibia bone marrows and induced the cells in vitro under specific conditions.By using two-dimensional gel electrophoresis(2-DE),we compared the protein profiles of MSCs before and after induced differentiation.We obtained 792 protein spots in the protein profile by 2-DE,and found that 74 spots changed significantly before and after the differentiation using PDQuest software,with 43 up-regulated and 31 down-regulated.We analyzed these 74 spots by a matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS) and by database searching,and found that they could be grouped into various classes,including cytoskeleton and structure proteins,growth factors,metabolic proteins,chaperone proteins,receptor proteins,cell cycle proteins,calcium binding proteins,and other proteins.These proteins also include neural and glial proteins,such as BDNF,CNTF and GFAP.The results may provide valuable proteomic information about the differentiation of MSCs into Schwann cell-like cells. 展开更多
关键词 one marrow STROMAL cells(MSCs) DIFFERENTIATION Schwann cell-like cellS proteomics
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Generation of developmentally competent oocytes and fertile mice from parthenogenetic embryonic stem cells 被引量:2
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作者 Chenglei Tian Linlin Liu +6 位作者 Ming Zeng Xiaoyan Sheng Dai Heng Lingling Wang Xiaoying Ye David L.Keefe Lin Liu 《Protein & Cell》 SCIE CSCD 2021年第12期947-964,共18页
Parthenogenetic embryos,created by activation and diploidization of oocytes,arrest at mid-gestation for defective paternal imprints,which impair placental development.Also,viable offspring has not been obtained withou... Parthenogenetic embryos,created by activation and diploidization of oocytes,arrest at mid-gestation for defective paternal imprints,which impair placental development.Also,viable offspring has not been obtained without genetic manipulation from parthenogenetic embryonic stem cells(pESCs)derived from parthenogenetic embryos,presumably attributable to their aberrant imprinting.We show that an unlimited number of oocytes can be derived from pESCs and produce healthy offspring.Moreover,normal expression of imprinted genes is found in the germ cells and the mice.pESCs exhibited imprinting consistent with exclusively maternal lineage,and higher X-chromosome activation compared to female ESCs derived from the same mouse genetic background.pESCs differentiated into primordial germ cell-like cells(PGCLCs)and formed oocytes following in vivo transplantation into kidney capsule that produced fertile pups and reconstituted ovarian endocrine function.The transcriptome and methylation of imprinted and X-linked genes in pESC-PGCLCs closely resembled those of in vivo produced PGCs,consistent with efficient reprogramming of methylation and genomic imprinting.These results demonstrate that amplification of germ cells through parthenogenesis faithfully maintains maternal imprinting,offering a promising route for deriving functional oocytes and having potential in rebuilding ovarian endocrine function. 展开更多
关键词 parthenogenetic embryonic stem cells primordial germ cell-like cells IMPRINTING MEIOSIS OOCYTES
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Modelling in vitro gametogenesis using induced pluripotent stem cells:a review
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作者 Maria Victoria Romualdez‑Tan 《Cell Regeneration》 CAS 2023年第1期53-66,共14页
In vitro gametogenesis(IVG)has been a topic of great interest in recent years not only because it allows for further exploration of mechanisms of germ cell development,but also because of its prospect for innovative m... In vitro gametogenesis(IVG)has been a topic of great interest in recent years not only because it allows for further exploration of mechanisms of germ cell development,but also because of its prospect for innovative medical applications especially for the treatment of infertility.Elucidation of the mechanisms underlying gamete development in vivo has inspired scientists to attempt to recapitulate the entire process of gametogenesis in vitro.While earlier studies have established IVG methods largely using pluripotent stem cells of embryonic origin,the scarcity of sources for these cells and the ethical issues involved in their use are serious limitations to the progress of IVG research especially in humans.However,with the emergence of induced pluripotent stem cells(iPSCs)due to the revolutionary discovery of dedifferentiation and reprogramming factors,IVG research has progressed remarkably in the last decade.This paper extensively reviews developments in IVG using iPSCs.First,the paper presents key concepts from groundwork studies on IVG including earlier researches demonstrating that IVG methods using embryonic stem cells(ESCs)also apply when using iPSCs.Techniques for the derivation of iPSCs are briefly discussed,highlighting the importance of generating transgene-free iPSCs with a high capacity for germline transmission to improve efficacy when used for IVG.The main part of the paper discusses recent advances in IVG research using iPSCs in various stages of gametogenesis.In addition,current clinical applications of IVG are presented,and potential future applications are discussed.Although IVG is still faced with many challenges in terms of technical issues,as well as efficacy and safety,novel IVG methodologies are emerging,and IVG using iPSCs may usher in the next era of reproductive medicine sooner than expected.This raises both ethical and social concerns and calls for the scientific community to cautiously develop IVG technology to ensure it is not only efficacious but also safe and adheres to social and et 展开更多
关键词 In-vitro gametogenesis Artificial gametes Germ cell derivation Primordial germ cell-like cells Induced pluripotent stem cells
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Division and k-th root theorems for Q-manifolds
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作者 Taras BANAKH Duan REPOV 《Science China Mathematics》 SCIE 2007年第3期313-324,共12页
We prove that a locally compact ANR-space X is a Q-manifold if and only if it has the Disjoint Disk Property (DDP), all points of X are homological Z∞-points and X has the countable-dimensional approximation property... We prove that a locally compact ANR-space X is a Q-manifold if and only if it has the Disjoint Disk Property (DDP), all points of X are homological Z∞-points and X has the countable-dimensional approximation property (cd-AP), which means that each map f:K→X of a compact polyhedron can be approximated by a map with the countable-dimensional image. As an application we prove that a space X with DDP and cd-AP is a Q-manifold if some finite power of X is a Q-manifold. If some finite power of a space X with cd-AP is a Q-manifold, then X2 and X×[0,1] are Q-manifolds as well. We construct a countable familyχof spaces with DDP and cd-AP such that no space X∈χis homeomorphic to the Hilbert cube Q whereas the product X×Y of any different spaces X, Y∈χis homeomorphic to Q. We also show that no uncountable familyχwith such properties exists. 展开更多
关键词 Hilbert cube Cantor cube Tychonov cube ANR infinite-dimensional manifold Disjoint Disk Property cell-like map 57N20 54F65 55N10 58B05 57N60
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Stem cell-like memory T cells:Role in viral infections and autoimmunity
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作者 Meenakshi Sachdeva Shivangi Taneja Naresh Sachdeva 《World Journal of Immunology》 2023年第2期11-22,共12页
Stem cell-like memory T(TSCM)cells possess stem cell properties including multipotency and self-renewal and are being recognized as emerging players in various human diseases.Advanced technologies such as multiparamet... Stem cell-like memory T(TSCM)cells possess stem cell properties including multipotency and self-renewal and are being recognized as emerging players in various human diseases.Advanced technologies such as multiparametric flowcytometry and single cell sequencing have enabled their identification and molecular characterization.In case of chronic viral diseases such as human immunodeficiency virus-1,CD4+T_(SCM) cells,serve as major reservoirs of the latent virus.However,during immune activation and functional exhaustion of effector T cells,these cells also possess the potential to replenish the pool of functional effector cells to curtail the infection.More recently,these cells are speculated to play important role in protective immunity following acute viral infections such as coronavirus disease 2019 and might be amenable for therapeutics by ex vivo expansion.Similarly,studies are also investigating their pathological role in driving autoimmune responses.However,there are several gaps in the understanding of the role of T_(SCM) cells in viral and autoimmune diseases to make them potential therapeutic targets.In this minireview,we have attempted an updated compilation of the dyadic role of these complex T_(SCM) cells during such human diseases along with their biology and transcriptional programs. 展开更多
关键词 Stem cell-like memory T cells Viral infections Autoimmune diseases Effector T cells Memory T cells
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Immune phenotypes of prostate cancer cells: Evidence of epithelial immune cell-like transition? 被引量:1
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作者 Dong Lin Xinya Wang +3 位作者 Stephen Yiu Chuen Choi Xinpei Ci Xin Dong Yuzhuo Wang 《Asian Journal of Urology》 2016年第4期195-202,共8页
Prostate cancers(PCa)have been reported to actively suppress antitumor immune responses by creating an immune-suppressive microenvironment.There is mounting evidence that PCas may undergo an‘‘Epithelial Immune Cell-... Prostate cancers(PCa)have been reported to actively suppress antitumor immune responses by creating an immune-suppressive microenvironment.There is mounting evidence that PCas may undergo an‘‘Epithelial Immune Cell-like Transition’’(EIT)by expressing molecules conventionally associated with immune cells(e.g.,a variety of cytokines/receptors,immune transcription factors,Ig motifs,and immune checkpoint molecules),which subsequently results in the suppression of anti-cancer immune activity within the tumor microenvironment.Recent progress within the field of immune therapy has underscored the importance of immune checkpoint molecules in cancer development,thus leading to the development of novel immunotherapeutic approaches.Here,we review the expression of select immune checkpoint molecules in PCa epithelial and associated immune cells,with particular emphasis on clinical data supporting the concept of an EIT-mediated phenotype in PCa.Furthermore,we summarize current advances in anti-immune checkpoint therapies,and provide perspectives on their potential applicability. 展开更多
关键词 Prostate cancer Immune checkpoint Epithelial immune cell-like transition Immune suppression Immune therapy
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Screening the Optimal Patterned Surfaces Consisting of Cell Morphology Mimicking Micro-pillars and Nanotube Arrays for the Design of Titanium Implants#br# 被引量:1
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作者 Ping Zhou Hongjiao Li +5 位作者 Feifei Mao Hongxin Huang Siqi Long Fei He Jing Chen Shicheng Wei 《Journal of Bionic Engineering》 SCIE EI CSCD 2021年第2期361-374,共14页
Micron/nano scale topographic modification has been a significant focus of interest in current titanium(Ti)surface design.However,the influence of micron/nano structured surface on cell or bacterium behavior on the Ti... Micron/nano scale topographic modification has been a significant focus of interest in current titanium(Ti)surface design.However,the influence of micron/nano structured surface on cell or bacterium behavior on the Ti implant has rarely been systematically evaluated.Moreover,except for popular microgrooves,little work has been carried out on the reaction of cells to the bionic structure.In this study,several micro-pillars mimicking cell morphology were prepared on Ti surfaces by lithography and contact printing(ICP)method,and they were further decorated with nanotube arrays by anodization technology.These surface modifications remarkablly increased the surface roughness of pristine Ti surface from 91.17 nm±5.57 nm to be more than 1000 nm,and reduced their water contact angles from 68.3°±0.7°to be 16.9°±2.4°.Then,the effects of these hierarchical micron/nano scale patterns on the behaviors of MG63 osteoblasts,L929 fibroblasts,SCC epithelial cells and P.gingivalis were studied,aiming to evaluate their performance in osseointegration,gingival epithelial sealing and antibacterial ability.Through an innovative scoring strategy,our findings showed that square micro-pillars with 6μm width and 2μm height combined with 85 nm diameter nanotubes was suitable for implant neck design,while square micro-pillars with 3μm width and 3.6μm height combined with 55 nm diameter nanotubes was the best for implant body design.Our study reveals the synergistic effect of the hierarchical micron/nano scale patterns on MG63 osteoblasts,L929 fibroblasts,SCC epithelial cells and P.gingivalis functions.It provides insight into the design of biomedical implant surfaces. 展开更多
关键词 hierarchical micron/nano design cell-like patterns nanotube arrays titanium implants implant osseointegration
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人体位改变对32项生化指标影响的研究 被引量:78
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作者 李素珍 林福禧 +4 位作者 沈波 樊锦秀 李伯利 罗凌飞 李学信 《中华检验医学杂志》 CAS CSCD 北大核心 2003年第2期107-109,共3页
目的 探索不同体位对生化指标的影响。方法  10 7名志愿者 ,经交叉同体配对设计 ,分冬、春、夏 3批 ,抽坐、卧两种姿势同一部位的血液 ,其中 19名分别抽站、坐 15min、卧 15min、30min、再坐 15min标本 ,在自动生化分析仪上配对分析 3... 目的 探索不同体位对生化指标的影响。方法  10 7名志愿者 ,经交叉同体配对设计 ,分冬、春、夏 3批 ,抽坐、卧两种姿势同一部位的血液 ,其中 19名分别抽站、坐 15min、卧 15min、30min、再坐 15min标本 ,在自动生化分析仪上配对分析 32项指标。结果 以卧位为基线与坐位相比 ,2 5项生化检测结果差异有非常显著意义 (P <0 0 0 1) ,3项差异有显著意义 (P <0 0 5 ) ,从卧位到立位结果逐渐升高 ,依次为卧位 <坐位 <立位 ,平均升高 11 49% ,最高升 5 5 39%。卧位后坐 15min ,各指标可回复到原坐位的 94%以上 ,季节、年龄与体位改变无关 ,与性别有关 ,无机磷 (IP)、尿酸 (UA)、肌酐(Cre)、葡萄糖 (Glu)差异无显著意义 (P >0 0 5 )。结论 体位改变能引起 2 8项生化指标的显著生理变异 ,与蛋白质相关的项目变异尤为显著 ,致使正常边缘数值出现异常结果。提示在分析实验数据时不要忽视体位因素 ,以免引起误导。 展开更多
关键词 体位 生物化学指标 实验数据
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银杏二萜内酯葡胺注射液联合曲克芦丁脑蛋白水解物注射液治疗急性脑梗死的疗效观察 被引量:22
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作者 李洪君 张广丽 《广西医学》 CAS 2021年第17期2062-2066,共5页
目的观察银杏二萜内酯葡胺注射液联合曲克芦丁脑蛋白水解物注射液治疗急性脑梗死的疗效。方法将80例急性脑梗死患者随机分为单药治疗组、联合治疗组,每组40例,两组患者均给予常规治疗及曲克芦丁脑蛋白水解物注射液治疗,联合治疗组加用... 目的观察银杏二萜内酯葡胺注射液联合曲克芦丁脑蛋白水解物注射液治疗急性脑梗死的疗效。方法将80例急性脑梗死患者随机分为单药治疗组、联合治疗组,每组40例,两组患者均给予常规治疗及曲克芦丁脑蛋白水解物注射液治疗,联合治疗组加用银杏二萜内酯葡胺注射液治疗,两组疗程均为14 d。观察两组患者治疗前后血清D-二聚体、纤维蛋白原(FIB)、肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(hs-CRP)、白细胞介素6(IL-6)、血小板反应蛋白1(TSP-1)、可溶性髓样细胞触发受体样转录因子1(sTLT-1)、神经营养因子(NTF)、神经生长因子(NGF)、神经元特异性烯醇化酶(NSE)水平及美国国立卫生研究院卒中量表(NIHSS)、Barthel指数评分,比较两组临床疗效。结果治疗后,两组患者血清D-二聚体、FIB、TNF-α、hs-CRP、IL-6、TSP-1、sTLT-1、NSE水平及NIHSS评分均低于治疗前,NTF、NGF水平及Barthel指数评分均高于治疗前,并且联合治疗组以上各指标改善优于单药治疗组(均P<0.05);联合治疗组治疗总有效率高于单药治疗组(P<0.05)。结论采用银杏二萜内酯葡胺注射液联合曲克芦丁脑蛋白水解物注射液治疗急性脑梗死,能改善患者凝血功能、神经功能,促进血液循环,降低机体炎症反应的发生,提高患者日常生活能力,疗效显著。 展开更多
关键词 急性脑梗死 银杏二萜内酯葡胺 曲克芦丁脑蛋白水解物 神经细胞 血小板反应蛋白1 可溶性髓样细胞触发受体样转录因子1
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类胰岛素生长因子-1及其受体mRNA在培养肌腱细胞内的表达 被引量:10
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作者 项舟 杨志明 +2 位作者 夏庆杰 张朝良 彭文珍 《中国修复重建外科杂志》 CAS CSCD 1997年第6期369-371,共3页
为了进一步探讨类胰岛素生长因子-1(IGF-1)受体系统在培养肌腱细胞群体生命活动中的变化,采用地高辛标记的IGF-1及其受体基因的寡核苷酸探针,对原代、第6代及第13代肌腱细胞RNA进行杂交,探测IGF-1及其受体... 为了进一步探讨类胰岛素生长因子-1(IGF-1)受体系统在培养肌腱细胞群体生命活动中的变化,采用地高辛标记的IGF-1及其受体基因的寡核苷酸探针,对原代、第6代及第13代肌腱细胞RNA进行杂交,探测IGF-1及其受体在上述细胞中的mRNA表达。结果发现,上述三种细胞均表达IGF-1受体mRNA;只有原代和第6代细胞表达IGF-1mRNA,而第13代细胞不表达IGF-1mRNA。提示,IGF-1mRNA不表达可能是导致第13代肌腱细胞增殖能力下降的原因之一。 展开更多
关键词 IGF-1 肌腱细胞 受体 MRNA 培养
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非血缘关系HLA全相合造血干细胞移植中供者-受者NK细胞KIR的研究 被引量:9
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作者 鲍晓晶 何军 +10 位作者 陈子兴 吴德沛 姚利 袁晓妮 岑建农 邱桥成 狄文英 张辉 张健 周晓华 徐惠新 《中华血液学杂志》 CAS CSCD 北大核心 2007年第8期510-513,共4页
目的研究自然杀伤细胞免疫球蛋白样受体(KIR)的生物学功能及供者抑制性 KIR 和受者 HLA 遗传背景在无关供者全相合造血干细胞移植(HSCT)中的作用。方法采用序列特异性引物聚合酶链反应(SSP-PCR)和序列特异性寡核苷酸探针聚合酶链反应(SS... 目的研究自然杀伤细胞免疫球蛋白样受体(KIR)的生物学功能及供者抑制性 KIR 和受者 HLA 遗传背景在无关供者全相合造血干细胞移植(HSCT)中的作用。方法采用序列特异性引物聚合酶链反应(SSP-PCR)和序列特异性寡核苷酸探针聚合酶链反应(SSOP-PCR)的方法,对中国造血干细胞捐献者资料库中提供的51对 HLA 全相合供、受者进行 KIR 及 HLA 分型,患者中急性淋巴细胞白血病 ALL 18例,慢性粒细胞白血病(CML)15例,急性髓系白血病(AML)10例及其他患者8例。结果 51对供者-受者均存在 2DL1、2DL2/L3、2DLA、3DL2和3DL3,基因频率均为1,96.7%的个体表达 KIR3DL1。供-受者中21.57%KIR 完全相同;78.43%KIR 不完全相同,而 KIR 不相同又分为受者KIR 基因型包含供者 KIR 为25.49%,供者 KIR 基因型包含受者 KIR 为27.45%。74.62%的供者KIR2DL1无受者 C2配体,5.91%的供者 KIR2DL2/L3无受者 C1配体,19.74%的供者 KIR3DL1无受者 Bw4配体,54.91%的供者 KIR3D12无受者 A3、A11配体。结论 KIR 独特型和 HLA-Ⅰ类抗原是独立遗传的。供者 KIR2DL1和 KIR3DL2是主要引起 NK 细胞异源活性的受体,供、受者的 KIR/HLA 不匹配可能在全相合的无关供者异基因 HSCT 中起着十分重要的作用。KIR 受体-配体模型可以更好地提示 HSCT 的预后。 展开更多
关键词 杀伤细胞免疫球蛋白样受体 全相合无关供者 造血干细胞移植 杀伤细胞 天然
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胃肠道透明细胞肉瘤临床病理分析并文献复习 被引量:10
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作者 黄会粉 刘倩 +4 位作者 步宏 陈敏 陈卉娇 林英英 张红英 《临床与实验病理学杂志》 CAS CSCD 北大核心 2014年第4期383-388,共6页
目的探讨胃肠道透明细胞肉瘤(clear cell sarcoma of the gastrointestinal tract,CCS-GI)的临床病理特征及遗传学特点。方法对1例CCS-GI进行组织学观察、免疫组化染色、荧光原位杂交(fluorescence in situ hybridization,FISH)检测,并... 目的探讨胃肠道透明细胞肉瘤(clear cell sarcoma of the gastrointestinal tract,CCS-GI)的临床病理特征及遗传学特点。方法对1例CCS-GI进行组织学观察、免疫组化染色、荧光原位杂交(fluorescence in situ hybridization,FISH)检测,并复习相关文献。结果患者女性,因"腹痛1周"入院,CT检查示右半结肠肿瘤,肉眼观察结肠黏膜面可见一溃疡型肿块,切面灰白、实性、质嫩,侵及全层。镜下见中等大小的圆形或卵圆形肿瘤细胞呈片状排列,肿瘤细胞间可见散在分布的破骨细胞样多核巨细胞。肿瘤细胞S-100蛋白弥漫阳性,HMB-45、Melan-A、CD117、CD1a及PCK均阴性。FISH检测结果示74%的肿瘤细胞存在EWSR1基因易位。结论 CCS-GI是一种特殊类型的胃肠道肿瘤,具有独特的组织学、免疫表型、超微结构及遗传学特征,该类肿瘤中的胃肠道透明细胞肉瘤样肿瘤亚型是否为一个独立的病变实体,尚需增加病例量进一步研究,包括细胞遗传学和分子生物学的相关研究。 展开更多
关键词 胃肠道肿瘤 胃肠道透明细胞肉瘤 胃肠道透明细胞肉瘤样肿瘤 软组织透明细胞肉瘤 遗传学
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紫草素通过调节PI3K/AKT途径抑制骨肉瘤生长和作用机制研究 被引量:9
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作者 杨志强 陈路 +1 位作者 张雅茜 夏先学 《中国比较医学杂志》 CAS 北大核心 2022年第1期68-74,96,共8页
目的研究紫草素(shikonin,SK)的抗骨肉瘤作用及其机制。方法不同浓度的SK处理人骨肉瘤U2OS和MG63细胞及人成骨细胞HFOB1.1924 h或用SK 1μmol/L分别处理24、48和72 h后,CCK-8检测细胞活性;SK 0、0.01、0.1、1μmol/L处理U2OS细胞,克隆... 目的研究紫草素(shikonin,SK)的抗骨肉瘤作用及其机制。方法不同浓度的SK处理人骨肉瘤U2OS和MG63细胞及人成骨细胞HFOB1.1924 h或用SK 1μmol/L分别处理24、48和72 h后,CCK-8检测细胞活性;SK 0、0.01、0.1、1μmol/L处理U2OS细胞,克隆形成实验检测细胞增殖;流式检测细胞凋亡,Western blot检测凋亡相关蛋白的表达;U2OS细胞分为对照组,SK1μmol/L组,PI3K激活剂胰岛素样生长因子1(IGF-1)组和SK1μmol/L+IGF-1组,Western blot检测磷脂酰肌醇3-激酶(PI3K)和蛋白激酶B(AKT)磷酸化、胱天蛋白酶(caspase-3,cas3)、cleaved cas3、Ki67及干细胞标记物SOX-2、OCT-4和Nanog的表达;细胞成球实验检测细胞成球能力。右侧腹皮下注射U2OS细胞构建移植瘤裸鼠模型并进行体内验证。结果不同浓度的SK对HFOB1.19细胞无明显毒性作用,而对U2OS和MG63细胞有显著毒性作用(P<0.05),且具有浓度和时间依赖性。紫草素0.1和1μmol/L可显著抑制U2OS细胞增殖和干细胞样特征,诱导细胞凋亡(P<0.05),并抑制PI3K和AKT蛋白的磷酸化(P<0.05)。IGF-1明显逆转紫草素对PI3K/AKT信号通路的抑制作用,以及对U2OS细胞增殖、凋亡,干细胞样特征的影响(P<0.05)。体内实验表明,紫草素能显著抑制肿瘤的生长(P<0.05),并下调瘤组织中p-AKT的表达(P<0.05)。结论紫草素对骨肉瘤U2OS细胞生长和干细胞样特征的抑制作用可能与抑制PI3K/AKT信号通路的激活有关。 展开更多
关键词 骨肉瘤 紫草素 增殖 细胞凋亡 干细胞样特征 磷酸肌醇3-激酶/蛋白激酶B
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