Juniperus procera Endl. is economically important timber species, but its populations are extremely small and fragmented in its natural habitat, thus, calling for immediate ex situ conservation. Here we examined the e...Juniperus procera Endl. is economically important timber species, but its populations are extremely small and fragmented in its natural habitat, thus, calling for immediate ex situ conservation. Here we examined the effects of seed sources and storage temperature on the longevity of Juniperus procera seed lots through collection and preservation of seeds in seed banks. Seeds were collected from nine sites across the species natural distribution in Ethiopia and stored in four warehouses： modern cold room （5℃）, mud house （15℃）, concrete block house （17℃ or corrugated iron house （20℃） for 42 months. Every three months, a random sample of stored seeds were drawn and tested for germination. A highly significant variation （p 0.01） in germination of stored seeds was observed among different storage environments, seed lots, and duration of storage. Over the storage period, seeds stored in the cold room had the highest mean percentage germination, followed by the mud house, corrugated house and blocket house. The cold room （41%） and the mud house （38%） maintained the same level of germination as the intitial germination of the seedlotds （42%）. The variation in longevity of stored seeds was significnatly correlated with the initial germination of seed lots （r 0.80; p 0.01）. Cold storage also resulted in enhancement of germination through its stratification effect that terminated the non-deepphysiological dormancy of juniper seeds. In conclusion, seed lots with good initial germination can be effectively stored in cold room （5℃） up to four years. In the absence of modern cold stores, mud houses can be used as a good alternative to store seeds at local level.展开更多
This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach（Lake Erie,Canada）.Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beac...This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach（Lake Erie,Canada）.Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beach showed that magnetite（～150μm） is the dominant magnetic mineral.Surficial magnetic susceptibility values defined three zones：a lakeward region close to the water line（Zone 1）,the upper swash zone（Zone 2） and the region landwards of the upper swash zone （Zone 3）.Zone 2 showed the highest bulk and mass susceptibility（κ,χ） and the highest mass percentage of smaller grain-size（250μm） fractions in the bulk sand sample.Susceptibility（i.e.κandχ） values decreased and grain size coarsened from Zone 2 lakewards（into Zone 1） and landwards （into Zone 3）,and correlated with the distribution of the heavy mineral assemblage,most probably reflecting preferential separation of large,less dense particles by waves and currents both along and across the beach.The eroded western section of Cedar Beach showed much higher concentrations of heavy minerals including magnetite,and finer sand grain sizes than the accreting eastern section, suggesting that magnetic techniques could be used as a rapid,cost-effective way of examining erosion along sensitive coastline areas.展开更多
Perfuorinated compounds(PFCs)are toxic and widely present in the environment,and therefore effective adsorbents are required to remove PFCs from environmental water.In the present study,a new type of fuorinated biomas...Perfuorinated compounds(PFCs)are toxic and widely present in the environment,and therefore effective adsorbents are required to remove PFCs from environmental water.In the present study,a new type of fuorinated biomass materials was synthesized via an ingenious fuorosilanization reaction.These adsorbents were applied for the adsorption of 13typical PFCs,including perfuorocarboxylic acids(PFCAs)and perfuorosulfonic acids(PFSAs).By comparing their adsorption performance,Fluorinated cedar slag(FCS)was discovered to have the best absorption efficiency and enabled highly efficient enrichment of PFCs.The adsorption recovery of FCS with the investigated PFCs is greater than 90%under the optimal adsorption condition.Ascribed to the high affinity of F-F sorbent-sorbate interaction,FCS had good adsorption capacities of PFCs from aqueous solution,with the maximum adsorption capacity of 15.80 mg/g for PFOS and 10.71 mg/g for PFOA,respectively.Moreover,the adsorption time could be achieved in a short time(8 min).Using the FCS absorbent,an innovative FCS-solid phase extraction assisted with high performance liquid chromatography-electrospray-tandem mass spectrometry(FCS-SPE-HPLC-ESI-MS/MS)method was first developed to sensitively detect PFCs in the environmental water samples.The intra-day and inter-day recovery rates of the 13 compounds ranged from 90.7%-104.3%,with the RSD of 2.1%-4.7%(intra-day)and 2.5%-8.5%(inter-day),respectively.This research demonstrates the potential of the newly fuoro-functionalized plant biomass to adsorb PFCs from environmental water,with the advantages of high adsorption efficiencies,high antiinterference,easy operation and low economic cost.展开更多
The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase...The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction(qRT-PCR)assay is pivotal for the early warning of the potential of zoonotic infectious diseases.Specific primers and probes were designed for the relatively conserved regions based on whole genome sequences of Langya virus(LayV),Mojiang virus(MojV),Nipah virus(NiV),and Cedar virus(CedV),followed by the establishment of a quadruple real-time fluorescence-based qRT-PCR detection method.No cross-reactivity was observed with other viral nucleic acids.The optimal linear detection range for LayV,MojV,NiV,and CedV was 10^(1)-10^(8)copies/μL,and the lower limit of detection was 10 copies/μL.Three different DNA concentrations of LayV,MojV,NiV,and CedV(10^(4),10^(5),and 10^(6)copies/μL)were tested 14 times,achieving good repeatability.The standard deviation of the cycle threshold values for each concentration was<0.5 and the coefficient of variation was<3%.Furthermore,the amplification efficiency of quadruple real-time fluorescence-based qRT-PCR was>90%,and the correlation coefficient was>0.99.The established quadru-ple real-time fluorescence-based qRT-PCR assay for the detection of LayV,MojV,NiV,and CedV exhibits good sensitivity,specificity,and repeatability.Therefore,it can be used to detect Henipavirus and other related clinical specimens.展开更多
This research examines the impact of wettability alteration on the end points of relative permeability,a crucial property of fluids and porous media that influences the displacement processes of immiscible fluids thro...This research examines the impact of wettability alteration on the end points of relative permeability,a crucial property of fluids and porous media that influences the displacement processes of immiscible fluids through such media.The estimation of the mobility ratio for oil recovery relies on these end points,which are influenced by connate water saturation and residual oil saturation.To investigate this relationship,carbonate rock is generally subjected to wettability alteration using surfactant agents,and core flooding is employed to determine the relative permeability before and after the alteration.The wettability of the rock is commonly assessed through contact angle measurements.Two surfactants,TritonX-100(Tx-100)and Cedar,were tested in reducing the wettability of the porous media for oil.The contact angle measurements revealed that Tx-100 was more effective for this purpose than Cedar.Furthermore,the relative permeability tests indicated that both surfactants decreased residual oil saturation,but Tx-100 also improved system pressure.In contrast,Cedar reduced residual oil saturation but increased system pressure,possibly because of its high viscosity.The results also demonstrate that injecting Tx-100 leads to a 14%increase in ultimate oil recovery compared with water injection,while Cedar injection increased the recovery factor by 5%.This difference may be attributed to the incomplete coverage of the pore wall by Cedar or its weaker chemical structure than Tx-100.Notably,in carbonate cores,neither non-ionic surfactant enhanced oil recovery.展开更多
Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carri...Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carried out among 1 660 primary and middle school students and 1 620 university students, including questionnaire investigation, nasal inspection by the same doctor and scratch test with the allergen extract of Cj. The pollen of Cf and Cj were observed by optical and electron microscope. The genes of chloroplast of them were analysed. Results ①The total positive rate of cedar pollen scratch test was 4.2%. 55 cases of nasal allergy were confirmed according to the criteria of diagnosis, in which 8 cases showed positive reaction to Cj and only 1 case was hypersentive to it. ②No significant distinction between Cf and Cj was found by optical and electron microscope and no difference in gene analysis was found. Conclusion\ ①Cedar pollinosis does exist in China. It′s low prevbalence is related to the low quantity of cedar pollen.②Cf and Cj are of the same family and genus. ③There may be an increasing tendency of cedar pollinosis in China.\;展开更多
Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have...Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have a long history and are still used in Yamagata Prefecture today. The purpose of this study was to determine the impact of a low severity controlled fire underneath Japanese cedar (Cryptomeria japonica) on brown forest soil (Cambisol). Japanese Cedar is the dominant species among plantations in Japan. We measured organic carbon and nitrogen content as well as changes in carbon (δ13C) and nitrogen (δ15N) stable isotope composition in a steep west facing slope under heavy precipitation (~2600 mm/a) and heavy snowfall (~3 to 4 m/a). The accumulation of Ctotal and Ntotal at the bottom of the slopes was remarkably higher at the slash and burned site than in the control forest site. After slash and burn δ15N isotopes in the slope in general became significantly lighter than in the control forest while the δ13C did not show any significant difference between the two sites except at the bottom of the slopes where δ13C was heavier in the forest. The results show that Ctotal and Ntotal values as well as the isotopes ratios of C and N change with decreasing elevation in the forest as well as in the burned site being consistent with leaching and erosion. The changes in soil nitrogen and carbon isotopes at the bottom of the slope appear to be related to the transport of material with different isotopic composition from the upper slope. The effect of the low severity fire (as part of the slash and burn practice) on soil organic carbon and nitrogen movement was enhanced by the steepness of the slopes and the high precipitation of Shonai region.展开更多
基金supported by the Ethiopian Institute of Agricultural Research Organization
文摘Juniperus procera Endl. is economically important timber species, but its populations are extremely small and fragmented in its natural habitat, thus, calling for immediate ex situ conservation. Here we examined the effects of seed sources and storage temperature on the longevity of Juniperus procera seed lots through collection and preservation of seeds in seed banks. Seeds were collected from nine sites across the species natural distribution in Ethiopia and stored in four warehouses： modern cold room （5℃）, mud house （15℃）, concrete block house （17℃ or corrugated iron house （20℃） for 42 months. Every three months, a random sample of stored seeds were drawn and tested for germination. A highly significant variation （p 0.01） in germination of stored seeds was observed among different storage environments, seed lots, and duration of storage. Over the storage period, seeds stored in the cold room had the highest mean percentage germination, followed by the mud house, corrugated house and blocket house. The cold room （41%） and the mud house （38%） maintained the same level of germination as the intitial germination of the seedlotds （42%）. The variation in longevity of stored seeds was significnatly correlated with the initial germination of seed lots （r 0.80; p 0.01）. Cold storage also resulted in enhancement of germination through its stratification effect that terminated the non-deepphysiological dormancy of juniper seeds. In conclusion, seed lots with good initial germination can be effectively stored in cold room （5℃） up to four years. In the absence of modern cold stores, mud houses can be used as a good alternative to store seeds at local level.
基金supported by funding from the 111 Project B07011 of Ministry of Education of China,the China Scholarship Council(CSC) to SWZ (NCIS No.2007103928)an NSERC grant to MTC. D.Chevalier is thanked for her help in sampling. Laboratory assistance was provided bv K.Kawasaki and S.Joshi
文摘This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach（Lake Erie,Canada）.Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beach showed that magnetite（～150μm） is the dominant magnetic mineral.Surficial magnetic susceptibility values defined three zones：a lakeward region close to the water line（Zone 1）,the upper swash zone（Zone 2） and the region landwards of the upper swash zone （Zone 3）.Zone 2 showed the highest bulk and mass susceptibility（κ,χ） and the highest mass percentage of smaller grain-size（250μm） fractions in the bulk sand sample.Susceptibility（i.e.κandχ） values decreased and grain size coarsened from Zone 2 lakewards（into Zone 1） and landwards （into Zone 3）,and correlated with the distribution of the heavy mineral assemblage,most probably reflecting preferential separation of large,less dense particles by waves and currents both along and across the beach.The eroded western section of Cedar Beach showed much higher concentrations of heavy minerals including magnetite,and finer sand grain sizes than the accreting eastern section, suggesting that magnetic techniques could be used as a rapid,cost-effective way of examining erosion along sensitive coastline areas.
基金supported by the National Natural Science Foundation of China (No.22276221,and 21675176)the Fundamental Research Funds for the Central Universities,and South-Central Minzu University (No.CZP21002)。
文摘Perfuorinated compounds(PFCs)are toxic and widely present in the environment,and therefore effective adsorbents are required to remove PFCs from environmental water.In the present study,a new type of fuorinated biomass materials was synthesized via an ingenious fuorosilanization reaction.These adsorbents were applied for the adsorption of 13typical PFCs,including perfuorocarboxylic acids(PFCAs)and perfuorosulfonic acids(PFSAs).By comparing their adsorption performance,Fluorinated cedar slag(FCS)was discovered to have the best absorption efficiency and enabled highly efficient enrichment of PFCs.The adsorption recovery of FCS with the investigated PFCs is greater than 90%under the optimal adsorption condition.Ascribed to the high affinity of F-F sorbent-sorbate interaction,FCS had good adsorption capacities of PFCs from aqueous solution,with the maximum adsorption capacity of 15.80 mg/g for PFOS and 10.71 mg/g for PFOA,respectively.Moreover,the adsorption time could be achieved in a short time(8 min).Using the FCS absorbent,an innovative FCS-solid phase extraction assisted with high performance liquid chromatography-electrospray-tandem mass spectrometry(FCS-SPE-HPLC-ESI-MS/MS)method was first developed to sensitively detect PFCs in the environmental water samples.The intra-day and inter-day recovery rates of the 13 compounds ranged from 90.7%-104.3%,with the RSD of 2.1%-4.7%(intra-day)and 2.5%-8.5%(inter-day),respectively.This research demonstrates the potential of the newly fuoro-functionalized plant biomass to adsorb PFCs from environmental water,with the advantages of high adsorption efficiencies,high antiinterference,easy operation and low economic cost.
基金supported by the National Key R&D Program of China(Grant No.2022YFC2601200).
文摘The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction(qRT-PCR)assay is pivotal for the early warning of the potential of zoonotic infectious diseases.Specific primers and probes were designed for the relatively conserved regions based on whole genome sequences of Langya virus(LayV),Mojiang virus(MojV),Nipah virus(NiV),and Cedar virus(CedV),followed by the establishment of a quadruple real-time fluorescence-based qRT-PCR detection method.No cross-reactivity was observed with other viral nucleic acids.The optimal linear detection range for LayV,MojV,NiV,and CedV was 10^(1)-10^(8)copies/μL,and the lower limit of detection was 10 copies/μL.Three different DNA concentrations of LayV,MojV,NiV,and CedV(10^(4),10^(5),and 10^(6)copies/μL)were tested 14 times,achieving good repeatability.The standard deviation of the cycle threshold values for each concentration was<0.5 and the coefficient of variation was<3%.Furthermore,the amplification efficiency of quadruple real-time fluorescence-based qRT-PCR was>90%,and the correlation coefficient was>0.99.The established quadru-ple real-time fluorescence-based qRT-PCR assay for the detection of LayV,MojV,NiV,and CedV exhibits good sensitivity,specificity,and repeatability.Therefore,it can be used to detect Henipavirus and other related clinical specimens.
文摘This research examines the impact of wettability alteration on the end points of relative permeability,a crucial property of fluids and porous media that influences the displacement processes of immiscible fluids through such media.The estimation of the mobility ratio for oil recovery relies on these end points,which are influenced by connate water saturation and residual oil saturation.To investigate this relationship,carbonate rock is generally subjected to wettability alteration using surfactant agents,and core flooding is employed to determine the relative permeability before and after the alteration.The wettability of the rock is commonly assessed through contact angle measurements.Two surfactants,TritonX-100(Tx-100)and Cedar,were tested in reducing the wettability of the porous media for oil.The contact angle measurements revealed that Tx-100 was more effective for this purpose than Cedar.Furthermore,the relative permeability tests indicated that both surfactants decreased residual oil saturation,but Tx-100 also improved system pressure.In contrast,Cedar reduced residual oil saturation but increased system pressure,possibly because of its high viscosity.The results also demonstrate that injecting Tx-100 leads to a 14%increase in ultimate oil recovery compared with water injection,while Cedar injection increased the recovery factor by 5%.This difference may be attributed to the incomplete coverage of the pore wall by Cedar or its weaker chemical structure than Tx-100.Notably,in carbonate cores,neither non-ionic surfactant enhanced oil recovery.
基金This paper is granted by the fund of Japanese-ChineseMedical A ssociation!( N o110 )
文摘Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carried out among 1 660 primary and middle school students and 1 620 university students, including questionnaire investigation, nasal inspection by the same doctor and scratch test with the allergen extract of Cj. The pollen of Cf and Cj were observed by optical and electron microscope. The genes of chloroplast of them were analysed. Results ①The total positive rate of cedar pollen scratch test was 4.2%. 55 cases of nasal allergy were confirmed according to the criteria of diagnosis, in which 8 cases showed positive reaction to Cj and only 1 case was hypersentive to it. ②No significant distinction between Cf and Cj was found by optical and electron microscope and no difference in gene analysis was found. Conclusion\ ①Cedar pollinosis does exist in China. It′s low prevbalence is related to the low quantity of cedar pollen.②Cf and Cj are of the same family and genus. ③There may be an increasing tendency of cedar pollinosis in China.\;
文摘Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have a long history and are still used in Yamagata Prefecture today. The purpose of this study was to determine the impact of a low severity controlled fire underneath Japanese cedar (Cryptomeria japonica) on brown forest soil (Cambisol). Japanese Cedar is the dominant species among plantations in Japan. We measured organic carbon and nitrogen content as well as changes in carbon (δ13C) and nitrogen (δ15N) stable isotope composition in a steep west facing slope under heavy precipitation (~2600 mm/a) and heavy snowfall (~3 to 4 m/a). The accumulation of Ctotal and Ntotal at the bottom of the slopes was remarkably higher at the slash and burned site than in the control forest site. After slash and burn δ15N isotopes in the slope in general became significantly lighter than in the control forest while the δ13C did not show any significant difference between the two sites except at the bottom of the slopes where δ13C was heavier in the forest. The results show that Ctotal and Ntotal values as well as the isotopes ratios of C and N change with decreasing elevation in the forest as well as in the burned site being consistent with leaching and erosion. The changes in soil nitrogen and carbon isotopes at the bottom of the slope appear to be related to the transport of material with different isotopic composition from the upper slope. The effect of the low severity fire (as part of the slash and burn practice) on soil organic carbon and nitrogen movement was enhanced by the steepness of the slopes and the high precipitation of Shonai region.
