We evaluated the cardioprotective effects of ginsenoside Rg1 in a diabetic rat model induced with high-fat diet and intraperitoneal injection of streptozotocin. Ginsenoside Rg1 was injected intraperitoneal y for 12 we...We evaluated the cardioprotective effects of ginsenoside Rg1 in a diabetic rat model induced with high-fat diet and intraperitoneal injection of streptozotocin. Ginsenoside Rg1 was injected intraperitoneal y for 12 weeks. Myocardial injury indices and oxidative stress markers were determined. Changes in cardiac ultrastructure were evaluated with transmission electron microscopy. Myocardial apoptosis was assessed via terminal deoxynucleotidyl transferase （TDT）-mediated DNA nick-end labeling （TUNEL） and immunohistochemistry. Ginsenoside Rg1 was as-sociated with a significant dose-dependent reduction in serum levels of creatinine kinase MB and cardiac troponin I, and lessened ultrastructural disorders in diabetic myocardium, relative to the untreated diabetic model rats. Also, compared with the untreated diabetic rats, significant reductions in serum and myocardial levels of malondialdehyde were noted in the ginsenoside Rg1-treated groups, and increased levels of the antioxidants （superoxide dismutase, catalase, and glutathione peroxidase） were detected. TUNEL staining indicated reduced myocardial apoptosis in ginsenoside Rg1-treated rats, which may be associated with reduced levels of caspase-3 （CASP3） and increased levels of B-cell lymphoma-extra-large （Bcl-xL） in the diabetic myocardium. Ginsenoside Rg1 treatment of diabetic rats was associated with reduced oxidative stress and attenuated myocardial apoptosis, suggesting that ginsenoside Rg1 may be of potential preventative and therapeutic value for cardiovascular injury in diabetic patients.展开更多
目的采用网络药理学与分子对接技术探讨生脉注射液的活性成分和治疗新型冠状病毒肺炎(COVID-19)的潜在作用机制。方法利用TCMSP及BATMAN-TCM数据库筛选生脉注射液的活性化合物,通过TCMSP及Targetnet在线数据库预测作用靶点,通过Cytoscap...目的采用网络药理学与分子对接技术探讨生脉注射液的活性成分和治疗新型冠状病毒肺炎(COVID-19)的潜在作用机制。方法利用TCMSP及BATMAN-TCM数据库筛选生脉注射液的活性化合物,通过TCMSP及Targetnet在线数据库预测作用靶点,通过Cytoscape3.7.1构建活性成分-作用靶点网络图;在GeneCards及OMIM数据库中以"coronavirus pneumonia"为关键词搜索冠状病毒肺炎相关疾病靶点,与生脉注射液化合物靶点进行交集筛选出共同靶点作为研究靶点,将共同靶点导入STRING数据库获取数据后在Cytoscape 3.7.1软件中构建蛋白质-蛋白质相互作用网络图;利用R语言进行GO(gene ontology)功能、KEGG(Kyoto encyclopedia of genes and genomes)通路富集分析,预测其作用机制,并构建"成分-靶点-通路"网络图;通过DiscoveryStudio 2.5软件对关键靶点进行分子对接分析。结果生脉注射液筛选得到22个活性化合物,分别为邻苯二甲酸二辛酯、β-谷甾醇、当归酰基戈米辛O、戈米辛A、戈米辛R、五味子丙素、内南五味子酯乙、长南酸、南五味子内酯、香蒲木脂素B、新杜松烷酸A、新杜松烷酸B、新杜松烷酸C、新南五味子木脂宁、五味子内酯A、五味子内酯E、五味子酸、尿苷、薯蓣皂苷元、鸟嘌呤核苷、N-反式阿魏酰酪胺、豆甾醇。相应作用靶点224个,与COVID-19的共同靶点16个,分别为CASP3、CASP8、PTGS2、BCL2、BAX、PRKCA、PTGS1、PIK3CG、F10、NOS3、DPP4、NOS2、TLR9、ACE、ICAM1、PRKCE,关键靶点涉及CASP3、PTGS2、NOS2、NOS3、ICAM1。GO功能富集分析得到生物过程(BP)条目771个,细胞组成(CC)条目11个,分子功能(MF)条目79个。KEGG通路富集分析筛选得到67条(P<0.05)信号通路,主要涉及糖尿病并发症AGE-RAGE信号通路、凋亡通路、P53信号通路、小细胞肺癌通路等。分子对接结果显示与关键靶点对接较好的成分有五味子内酯E、豆甾醇、N-反式阿魏酰酪胺。结论生脉注射�展开更多
目的探讨细胞凋亡通路相关基因CASP3和CASP7多态性与中国汉族人群噪声性听力损失(NIHL)易感性之间的关联。方法研究对象来自2014年杭州市1 549名噪声接触工人听力损失的横断面调查,采用1:1配对病例对照研究,病例组为电测听双耳高频平均...目的探讨细胞凋亡通路相关基因CASP3和CASP7多态性与中国汉族人群噪声性听力损失(NIHL)易感性之间的关联。方法研究对象来自2014年杭州市1 549名噪声接触工人听力损失的横断面调查,采用1:1配对病例对照研究,病例组为电测听双耳高频平均听阈>25 d B(A)的工人,对照组为性别、年龄、接噪工龄、工作岗位与病例匹配且双耳所有频段听阈均≤25 d B(A)的工人,共272对。PCR-LDR法检测2个SNP位点的基因型。采用多因素条件logistic回归模型分析SNP位点与NIHL的关联,并以叉生分析计算基因-环境交互作用。结果χ~2检验分析发现,CASP3基因rs1049216等位基因(C、T)频率组间分布有统计学差异(OR=0.68,95%CI=0.50~0.93),而基因型频率组间差异无统计学意义(P>0.05);CASP7基因rs10787498等位基因及基因型频率组间分布均无统计学意义(P>0.05);多因素条件logistic回归显示,CASP3基因rs1049216中,与野生基因型CC相比,突变基因型(CT+TT)为NIHL的保护因素(调整OR=0.65,95%CI=0.43~0.97);叉生分析表明rs1049216位点与文化程度、睡眠时间存在交互作用(P≤0.001),NIHL的危险性降低(OR值变小)。结论 CASP3基因rs1049216位点可能与中国汉族人群NIHL易感性有关,且可能与文化程度、睡眠时间存在交互作用。展开更多
文摘We evaluated the cardioprotective effects of ginsenoside Rg1 in a diabetic rat model induced with high-fat diet and intraperitoneal injection of streptozotocin. Ginsenoside Rg1 was injected intraperitoneal y for 12 weeks. Myocardial injury indices and oxidative stress markers were determined. Changes in cardiac ultrastructure were evaluated with transmission electron microscopy. Myocardial apoptosis was assessed via terminal deoxynucleotidyl transferase （TDT）-mediated DNA nick-end labeling （TUNEL） and immunohistochemistry. Ginsenoside Rg1 was as-sociated with a significant dose-dependent reduction in serum levels of creatinine kinase MB and cardiac troponin I, and lessened ultrastructural disorders in diabetic myocardium, relative to the untreated diabetic model rats. Also, compared with the untreated diabetic rats, significant reductions in serum and myocardial levels of malondialdehyde were noted in the ginsenoside Rg1-treated groups, and increased levels of the antioxidants （superoxide dismutase, catalase, and glutathione peroxidase） were detected. TUNEL staining indicated reduced myocardial apoptosis in ginsenoside Rg1-treated rats, which may be associated with reduced levels of caspase-3 （CASP3） and increased levels of B-cell lymphoma-extra-large （Bcl-xL） in the diabetic myocardium. Ginsenoside Rg1 treatment of diabetic rats was associated with reduced oxidative stress and attenuated myocardial apoptosis, suggesting that ginsenoside Rg1 may be of potential preventative and therapeutic value for cardiovascular injury in diabetic patients.
文摘目的采用网络药理学与分子对接技术探讨生脉注射液的活性成分和治疗新型冠状病毒肺炎(COVID-19)的潜在作用机制。方法利用TCMSP及BATMAN-TCM数据库筛选生脉注射液的活性化合物,通过TCMSP及Targetnet在线数据库预测作用靶点,通过Cytoscape3.7.1构建活性成分-作用靶点网络图;在GeneCards及OMIM数据库中以"coronavirus pneumonia"为关键词搜索冠状病毒肺炎相关疾病靶点,与生脉注射液化合物靶点进行交集筛选出共同靶点作为研究靶点,将共同靶点导入STRING数据库获取数据后在Cytoscape 3.7.1软件中构建蛋白质-蛋白质相互作用网络图;利用R语言进行GO(gene ontology)功能、KEGG(Kyoto encyclopedia of genes and genomes)通路富集分析,预测其作用机制,并构建"成分-靶点-通路"网络图;通过DiscoveryStudio 2.5软件对关键靶点进行分子对接分析。结果生脉注射液筛选得到22个活性化合物,分别为邻苯二甲酸二辛酯、β-谷甾醇、当归酰基戈米辛O、戈米辛A、戈米辛R、五味子丙素、内南五味子酯乙、长南酸、南五味子内酯、香蒲木脂素B、新杜松烷酸A、新杜松烷酸B、新杜松烷酸C、新南五味子木脂宁、五味子内酯A、五味子内酯E、五味子酸、尿苷、薯蓣皂苷元、鸟嘌呤核苷、N-反式阿魏酰酪胺、豆甾醇。相应作用靶点224个,与COVID-19的共同靶点16个,分别为CASP3、CASP8、PTGS2、BCL2、BAX、PRKCA、PTGS1、PIK3CG、F10、NOS3、DPP4、NOS2、TLR9、ACE、ICAM1、PRKCE,关键靶点涉及CASP3、PTGS2、NOS2、NOS3、ICAM1。GO功能富集分析得到生物过程(BP)条目771个,细胞组成(CC)条目11个,分子功能(MF)条目79个。KEGG通路富集分析筛选得到67条(P<0.05)信号通路,主要涉及糖尿病并发症AGE-RAGE信号通路、凋亡通路、P53信号通路、小细胞肺癌通路等。分子对接结果显示与关键靶点对接较好的成分有五味子内酯E、豆甾醇、N-反式阿魏酰酪胺。结论生脉注射�
文摘目的探讨细胞凋亡通路相关基因CASP3和CASP7多态性与中国汉族人群噪声性听力损失(NIHL)易感性之间的关联。方法研究对象来自2014年杭州市1 549名噪声接触工人听力损失的横断面调查,采用1:1配对病例对照研究,病例组为电测听双耳高频平均听阈>25 d B(A)的工人,对照组为性别、年龄、接噪工龄、工作岗位与病例匹配且双耳所有频段听阈均≤25 d B(A)的工人,共272对。PCR-LDR法检测2个SNP位点的基因型。采用多因素条件logistic回归模型分析SNP位点与NIHL的关联,并以叉生分析计算基因-环境交互作用。结果χ~2检验分析发现,CASP3基因rs1049216等位基因(C、T)频率组间分布有统计学差异(OR=0.68,95%CI=0.50~0.93),而基因型频率组间差异无统计学意义(P>0.05);CASP7基因rs10787498等位基因及基因型频率组间分布均无统计学意义(P>0.05);多因素条件logistic回归显示,CASP3基因rs1049216中,与野生基因型CC相比,突变基因型(CT+TT)为NIHL的保护因素(调整OR=0.65,95%CI=0.43~0.97);叉生分析表明rs1049216位点与文化程度、睡眠时间存在交互作用(P≤0.001),NIHL的危险性降低(OR值变小)。结论 CASP3基因rs1049216位点可能与中国汉族人群NIHL易感性有关,且可能与文化程度、睡眠时间存在交互作用。
