摘要
目的：观察针刺和神经生长因子（NGF）对脑瘫模型幼鼠智力恢复以及脑海马组织BCL-2、BAX、Casp3基因mRNA表达的影响。方法：采用缺血缺氧（HIBD）的方法制备幼鼠脑瘫的模型，幼鼠出生后7d称重、实验分组、造模。实验分为5组：正常组、假手术组、模型组、单纯针刺组、针刺＋NGF组。术后3～15d按计划给予相应干预。术后18d取材，用荧光定量PCR（SYBRGreenI染料法）检测其脑海马组织BCL-2、BAX、Casp3基因mRNA表达：用TUNEL法检测其脑皮质神经细胞凋亡情况。结果：与模型组相比，单纯针刺组和针刺＋NGF组幼鼠的脑细胞抗凋亡BCL-2基因mRNA表达A值均数更高；BAX、Casp3基因mRNA表达A值均数更低：脑皮质组织细胞凋亡指数均数更低，且针刺＋NGF组凋亡指数比单纯针刺组更低。结论：在抗脑神经细胞凋亡方面．针刺对HIBD幼鼠都有明显的治疗作用，加用NGF后效果更好。
Objective To observe the effects of acupuncture and nerve growth factor （NGF） on intelligence recovery and BCL-2,BAX,Casp3 gene mRNA of hippocampus tissues of rats model with cerebral palsy （CP）. Methods Using hypoxic ischemic method to make the model of newborn rats with CP. All the rats 7 days ＂,ffter born were weighed, modeled and grouped, divided into five groups： normal control group, sham operated control group, model control group （hypoxic ischemic brain damage group）, acupuncture group, acupuncture ＋ NGF group. Related intervention was given on scheme 3-15 days after operation, drawn at 18th day, expression of brain nerve cell BCL-2, BAX, Casp3 mRNA were detected by fluorescence quantitative PCR （SYBRGreen I） ; nerve cell apoptosis were detected by TUNEL. Results Compared with model control group, mRNA expression of BCL-2 in acupuncture group and acupuncture ＋ NGF group were higher; mRNA expression of BAX, Casp3 were lower; the apoptosis index of acupuncture group and acupuncture ＋ NGF group were lower, and acupuncture group was lower than and acupuncture ＋ NGF group. Conclusion Acupuncture is valid in anti-apoptosis, acupuncture adding NGF is better than acupuncture only.
出处
《实用医学杂志》
CAS
北大核心
2014年第1期33-36,共4页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:30740074)
