目的探讨心肌肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)中的表达及其临床意义。方法选取2011年1月至2013年12月临朐县人民医院收治的50例AMI患者作为AMI组,其中发病时间<4 h 21例,发病时间≥4 h 29例。另外选择同期健康体检...目的探讨心肌肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)中的表达及其临床意义。方法选取2011年1月至2013年12月临朐县人民医院收治的50例AMI患者作为AMI组,其中发病时间<4 h 21例,发病时间≥4 h 29例。另外选择同期健康体检者50例作为健康对照组。统计受试者血清c My BP-C、心脏肌钙蛋白I(c Tn I)、肌红蛋白(Myo)水平,并分析AMI患者血清c My BP-C、c Tn I、Myo与年龄、发病时间、体质指数的相关性。结果 AMI组血清c My BP-C、c Tn I、Myo水平均高于健康对照组[(106±16)μg/L比(18±6)μg/L、(10.76±3.32)μg/L比(0.031±0.003)μg/L、(122±33)μg/L比(23±5)μg/L],差异有统计学意义(P<0.01)。发病时间≥4 h组患者血c My BP-C、Myo、c Tn I水平高于发病时间<4 h组[(109±17)μg/L比(76±9)μg/L,(11.67±3.72)μg/L比(0.042±0.006)μg/L,(130±33)μg/L比(93±16)μg/L,P<0.01]。PCI术后患者血c My BP-C水平低于入院时[(59±11)μg/L比(107±16)μg/L,P<0.01],PCI术后患者血c Tn I、Myo水平高于入院时[(33±6)μg/L比(11±3)μg/L,(136±35)μg/L比(122±33)μg/L,P<0.01];血c My BP-C、c Tn I、Myo与AMI发病时间均呈正相关(P<0.05);血c My BP-C、c Tn I、Myo之间均呈正相关(P<0.05)。结论 c My BP-C可以作为AMI早期诊断的指标之一,还可作为评价PCI术早期效果的指标。展开更多
TNNI3K is a cardiac-specific and cardiac troponin I(cT n I)-interacting MAP kinase, known to play important roles in promoting cardiac differentiation, maintenance of beating rhythm and contractual force. The molecula...TNNI3K is a cardiac-specific and cardiac troponin I(cT n I)-interacting MAP kinase, known to play important roles in promoting cardiac differentiation, maintenance of beating rhythm and contractual force. The molecular structure of TNNI3 K contains three kinds of domain: a seven or ten NH2-terminal ankyrin repeat domain followed by a protein kinase domain and a COOH-terminal serine-rich domain. There are many binding sites in the structure of TNNI3 K for binding to ATP, magnesium, nucleotide, protein kinase C, antioxidant protein 1(AOP-1) and cT n I, indicating TNNI3 K has many interacting partners. This review summarizes the evidence, hypothesis and significance of TNNI3 K interacting with TNNI3 and its other putative interaction partners. From the literature, the interaction partners of TNNI3 K are divided into 2 types following their phenotypic pattern of functions, positive interaction(to increase the cardiac performance) or negative interaction(to suppress the cardiac performance). Following their binding sites, it also can be divided into other 2 types: binding to C-terminal domain(e.g., cT n I) or binding to both ankyrin repeat domain and C-terminal domains(AOP-1).To date, a well understood partner of TNNI3 K is cT nI, from the molecular structure, physiological function, mechanisms and its significance in some physiological and pathophysiological conditions. There are many reasons to believe that, with more understanding on the TNNI3 K interacting with its partners, we can understand more roles of TNNI3 K in some cardiac diseases.展开更多
目的分析血清心脏型肌球蛋白结合蛋白C(cMyBP-C)、肌红蛋白(Myo)表达与老年急性心肌梗死(AMI)患者经皮冠脉介入术(PCI)后心肌无复流的关系。方法回顾性分析,收集医院2018年3月至2020年5月接受PCI手术治疗的53例术后心肌无复流老年AMI患...目的分析血清心脏型肌球蛋白结合蛋白C(cMyBP-C)、肌红蛋白(Myo)表达与老年急性心肌梗死(AMI)患者经皮冠脉介入术(PCI)后心肌无复流的关系。方法回顾性分析,收集医院2018年3月至2020年5月接受PCI手术治疗的53例术后心肌无复流老年AMI患者资料,纳为心肌无复流组,另收集同期接受PCI术治疗的53例术后心肌复流老年AMI患者资料,纳为心肌复流组。全部老年AMI患者在入院时、PCI术后48 h均接受血清cMyBP-C、Myo检测,相关实验室检测结果、病例资料等完整;统计并比较两组相关基线资料及血清cMyBP-C、Myo水平,分析血清cMyBP-C、Myo表达与老年AMI患者PCI术后心肌无复流的关系。结果 106例老年AMI患者PCI术后48 h cMyBP-C、Myo水平均较入院时降低,差异有统计学意义(P<0.05);心肌无复流老年AMI患者入院时心肌肌钙蛋白I(cTnI)、cMyBP-C、Myo水平均高于心肌复流患者,差异有统计学意义(P<0.05);组间其他基线资料比较差异无统计学意义(P>0.05);经Logistic回归分析结果显示,cMyBP-C、Myo异常表达可能与老年AMI患者PCI术后心肌无复流有关,cMyBP-C、Myo过表达可能是老年AMI患者PCI术后心肌无复流的风险因子(OR>1,P<0.05);绘制ROC曲线发现,血清cMyBP-C、Myo单一及联合预测老年AMI患者PCI术后心肌无复流风险的AUC>0.80,均有一定预测价值。结论老年AMI患者PCI术后心肌无复流可能与血清cMyBP-C、Myo过表达有关,未来可考虑检测患者入院时血清cMyBP-C、Myo表达,辅助评估老年AMI患者PCI术后心肌无复流风险。展开更多
Since its discovery, myosin-binding protein C (cMyBP-C) has become a protein of interest clinically. With emergence of new methodologies and technologies, the structure and functions of cMyBP-C from different aspects ...Since its discovery, myosin-binding protein C (cMyBP-C) has become a protein of interest clinically. With emergence of new methodologies and technologies, the structure and functions of cMyBP-C from different aspects can be studied, enabling us to better understand its involvement in certain cardiac conditions. Studying its kinetics of release and clearance from the circulation and by comparing to other conventional biomarkers, it has been reported that cMyBP-C is eligible to be a novel biomarker for several cardiac conditions. Moreover, studying the genetics and their involvement in pathogenic mechanisms has opened the ideas for potential therapeutic strategies. More and more researches are constantly being done to better understand the role of cMyBP-C in dilated cardiomyopathy (DCM). The importance of cMyBP-C to the heart is still actively being investigated. Its presence is however crucial for sarcomere organization and proper regulation of cardiac contraction during systole and complete relaxation during diastole. Genetic mutation in cMyBP-C has been linked to cardiac conditions including hypertrophic and dilated cardiomyopathies. Around 350 types of mutations have already been documented leading to various cardiac conditions and abnormalities. Analyzing human heart samples has enabled us to better understand the importance of cMyBP-C and how its mutations lead to inherited cardiomyopathies. It is therefore necessary to have an update about the research progress of cMyBP-C in relation to DCM and other cardiac conditions.展开更多
目的探讨血清心脏肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)患者诊断中的价值。方法选择2014年3月至2015年11月心血管内科收治的AMI患者62例作为病例组,选取正常体检者60例作为对照组。采用双抗体夹心酶联免疫吸附试验(ELISA)...目的探讨血清心脏肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)患者诊断中的价值。方法选择2014年3月至2015年11月心血管内科收治的AMI患者62例作为病例组,选取正常体检者60例作为对照组。采用双抗体夹心酶联免疫吸附试验(ELISA)法检测血清c My BP-C浓度。比较AMI组与对照组间c My BP-C、肌钙蛋白Ⅰ(c TnⅠ)、肌酸激酶同工酶(CK-MB)和肌红蛋白(Myo)浓度的差异,分析AMI组c My BP-C与c TnⅠ、CKMB和Myo的相关性,同时分析发病时间小于4 h的AMI患者入院时血清c My BP-C和c TnⅠ浓度与对照组的差异,比较急诊经皮冠状动脉介入(PCI)术后12 h与入院时血清c My BP-C和c TnⅠ的差异。结果 AMI组患者血清c My BP-C、c TnⅠ、CK-MB和Myo浓度较对照组均明显升高(P<0.05)。相关性分析显示,AMI组患者c My BP-C浓度与c TnⅠ、CK-MB和Myo浓度均存在正相关(分别为r=0.876、P<0.05;r=0.632、P<0.05和r=0.903、P<0.05)。发病时间小于4 h的AMI患者入院时血清c My BP-C浓度较对照组明显升高(P<0.05),而血清c TnⅠ浓度与对照组比较差异无统计学意义(P>0.05)。行急诊PCI术后12 h血清c My BP-C浓度较入院时明显下降,而c TnⅠ浓度较入院时明显升高(P<0.05)。结论 AMI患者入院时血清c My BP-C、c TnⅠ、CK-MB和Myo浓度较对照组均显著升高且c My BP-C浓度与c TnⅠ、CK-MB和Myo浓度均存在正相关;c My BP-C在发病4 h内即开始升高,提示c My BP-C可以作为诊断AMI的早期生化标志物。AMI患者行急诊PCI术后12 h血清c My BP-C浓度较入院时明显下降,表明c My BP-C可以作为评估PCI术效果的早期指标。展开更多
文摘目的探讨心肌肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)中的表达及其临床意义。方法选取2011年1月至2013年12月临朐县人民医院收治的50例AMI患者作为AMI组,其中发病时间<4 h 21例,发病时间≥4 h 29例。另外选择同期健康体检者50例作为健康对照组。统计受试者血清c My BP-C、心脏肌钙蛋白I(c Tn I)、肌红蛋白(Myo)水平,并分析AMI患者血清c My BP-C、c Tn I、Myo与年龄、发病时间、体质指数的相关性。结果 AMI组血清c My BP-C、c Tn I、Myo水平均高于健康对照组[(106±16)μg/L比(18±6)μg/L、(10.76±3.32)μg/L比(0.031±0.003)μg/L、(122±33)μg/L比(23±5)μg/L],差异有统计学意义(P<0.01)。发病时间≥4 h组患者血c My BP-C、Myo、c Tn I水平高于发病时间<4 h组[(109±17)μg/L比(76±9)μg/L,(11.67±3.72)μg/L比(0.042±0.006)μg/L,(130±33)μg/L比(93±16)μg/L,P<0.01]。PCI术后患者血c My BP-C水平低于入院时[(59±11)μg/L比(107±16)μg/L,P<0.01],PCI术后患者血c Tn I、Myo水平高于入院时[(33±6)μg/L比(11±3)μg/L,(136±35)μg/L比(122±33)μg/L,P<0.01];血c My BP-C、c Tn I、Myo与AMI发病时间均呈正相关(P<0.05);血c My BP-C、c Tn I、Myo之间均呈正相关(P<0.05)。结论 c My BP-C可以作为AMI早期诊断的指标之一,还可作为评价PCI术早期效果的指标。
文摘TNNI3K is a cardiac-specific and cardiac troponin I(cT n I)-interacting MAP kinase, known to play important roles in promoting cardiac differentiation, maintenance of beating rhythm and contractual force. The molecular structure of TNNI3 K contains three kinds of domain: a seven or ten NH2-terminal ankyrin repeat domain followed by a protein kinase domain and a COOH-terminal serine-rich domain. There are many binding sites in the structure of TNNI3 K for binding to ATP, magnesium, nucleotide, protein kinase C, antioxidant protein 1(AOP-1) and cT n I, indicating TNNI3 K has many interacting partners. This review summarizes the evidence, hypothesis and significance of TNNI3 K interacting with TNNI3 and its other putative interaction partners. From the literature, the interaction partners of TNNI3 K are divided into 2 types following their phenotypic pattern of functions, positive interaction(to increase the cardiac performance) or negative interaction(to suppress the cardiac performance). Following their binding sites, it also can be divided into other 2 types: binding to C-terminal domain(e.g., cT n I) or binding to both ankyrin repeat domain and C-terminal domains(AOP-1).To date, a well understood partner of TNNI3 K is cT nI, from the molecular structure, physiological function, mechanisms and its significance in some physiological and pathophysiological conditions. There are many reasons to believe that, with more understanding on the TNNI3 K interacting with its partners, we can understand more roles of TNNI3 K in some cardiac diseases.
文摘目的分析血清心脏型肌球蛋白结合蛋白C(cMyBP-C)、肌红蛋白(Myo)表达与老年急性心肌梗死(AMI)患者经皮冠脉介入术(PCI)后心肌无复流的关系。方法回顾性分析,收集医院2018年3月至2020年5月接受PCI手术治疗的53例术后心肌无复流老年AMI患者资料,纳为心肌无复流组,另收集同期接受PCI术治疗的53例术后心肌复流老年AMI患者资料,纳为心肌复流组。全部老年AMI患者在入院时、PCI术后48 h均接受血清cMyBP-C、Myo检测,相关实验室检测结果、病例资料等完整;统计并比较两组相关基线资料及血清cMyBP-C、Myo水平,分析血清cMyBP-C、Myo表达与老年AMI患者PCI术后心肌无复流的关系。结果 106例老年AMI患者PCI术后48 h cMyBP-C、Myo水平均较入院时降低,差异有统计学意义(P<0.05);心肌无复流老年AMI患者入院时心肌肌钙蛋白I(cTnI)、cMyBP-C、Myo水平均高于心肌复流患者,差异有统计学意义(P<0.05);组间其他基线资料比较差异无统计学意义(P>0.05);经Logistic回归分析结果显示,cMyBP-C、Myo异常表达可能与老年AMI患者PCI术后心肌无复流有关,cMyBP-C、Myo过表达可能是老年AMI患者PCI术后心肌无复流的风险因子(OR>1,P<0.05);绘制ROC曲线发现,血清cMyBP-C、Myo单一及联合预测老年AMI患者PCI术后心肌无复流风险的AUC>0.80,均有一定预测价值。结论老年AMI患者PCI术后心肌无复流可能与血清cMyBP-C、Myo过表达有关,未来可考虑检测患者入院时血清cMyBP-C、Myo表达,辅助评估老年AMI患者PCI术后心肌无复流风险。
文摘Since its discovery, myosin-binding protein C (cMyBP-C) has become a protein of interest clinically. With emergence of new methodologies and technologies, the structure and functions of cMyBP-C from different aspects can be studied, enabling us to better understand its involvement in certain cardiac conditions. Studying its kinetics of release and clearance from the circulation and by comparing to other conventional biomarkers, it has been reported that cMyBP-C is eligible to be a novel biomarker for several cardiac conditions. Moreover, studying the genetics and their involvement in pathogenic mechanisms has opened the ideas for potential therapeutic strategies. More and more researches are constantly being done to better understand the role of cMyBP-C in dilated cardiomyopathy (DCM). The importance of cMyBP-C to the heart is still actively being investigated. Its presence is however crucial for sarcomere organization and proper regulation of cardiac contraction during systole and complete relaxation during diastole. Genetic mutation in cMyBP-C has been linked to cardiac conditions including hypertrophic and dilated cardiomyopathies. Around 350 types of mutations have already been documented leading to various cardiac conditions and abnormalities. Analyzing human heart samples has enabled us to better understand the importance of cMyBP-C and how its mutations lead to inherited cardiomyopathies. It is therefore necessary to have an update about the research progress of cMyBP-C in relation to DCM and other cardiac conditions.
文摘目的探讨血清心脏肌球蛋白结合蛋白C(c My BP-C)在急性心肌梗死(AMI)患者诊断中的价值。方法选择2014年3月至2015年11月心血管内科收治的AMI患者62例作为病例组,选取正常体检者60例作为对照组。采用双抗体夹心酶联免疫吸附试验(ELISA)法检测血清c My BP-C浓度。比较AMI组与对照组间c My BP-C、肌钙蛋白Ⅰ(c TnⅠ)、肌酸激酶同工酶(CK-MB)和肌红蛋白(Myo)浓度的差异,分析AMI组c My BP-C与c TnⅠ、CKMB和Myo的相关性,同时分析发病时间小于4 h的AMI患者入院时血清c My BP-C和c TnⅠ浓度与对照组的差异,比较急诊经皮冠状动脉介入(PCI)术后12 h与入院时血清c My BP-C和c TnⅠ的差异。结果 AMI组患者血清c My BP-C、c TnⅠ、CK-MB和Myo浓度较对照组均明显升高(P<0.05)。相关性分析显示,AMI组患者c My BP-C浓度与c TnⅠ、CK-MB和Myo浓度均存在正相关(分别为r=0.876、P<0.05;r=0.632、P<0.05和r=0.903、P<0.05)。发病时间小于4 h的AMI患者入院时血清c My BP-C浓度较对照组明显升高(P<0.05),而血清c TnⅠ浓度与对照组比较差异无统计学意义(P>0.05)。行急诊PCI术后12 h血清c My BP-C浓度较入院时明显下降,而c TnⅠ浓度较入院时明显升高(P<0.05)。结论 AMI患者入院时血清c My BP-C、c TnⅠ、CK-MB和Myo浓度较对照组均显著升高且c My BP-C浓度与c TnⅠ、CK-MB和Myo浓度均存在正相关;c My BP-C在发病4 h内即开始升高,提示c My BP-C可以作为诊断AMI的早期生化标志物。AMI患者行急诊PCI术后12 h血清c My BP-C浓度较入院时明显下降,表明c My BP-C可以作为评估PCI术效果的早期指标。
文摘目的 分析心肌肌球蛋白结合蛋白C(cMyBP-C)在急性心肌梗死(AMI)、心肌缺血再灌注损伤模型猪中的表达及意义。方法 本实验时间为2020年6-12月。将9头试验用滇南小耳猪随机分为假手术组(开胸后打开心包,不结扎冠状动脉,n=1)、AMI组〔开胸后打开心包,结扎左前降支(LAD)中远段血管,n=4〕、心肌缺血再灌注损伤组(开胸后打开心包,结扎LAD中远段血管3 h后再开放,n=4)。观察各组滇南小耳猪手术前后心率及心电图表现、术后心脏组织病理切片,检测假手术组、AMI组术前及术后0.5、1.0、2.0、3.0、4.0、6.0、8.0、10.0、12.0、18.0、24.0 h心肌肌钙蛋白Ⅰ(cTnⅠ)、cMyBP-C水平,心肌缺血再灌注损伤组术前及术后0.5、1.0、2.0、3.0、3.5、4.5、5.5、6.5、7.5、9.5、11.5、13.5、15.5、21.5、27.5 h cTnⅠ、cMyBP-C水平。结果 假手术组无死亡发生;AMI组有1头滇南小耳猪死亡;心肌缺血再灌注损伤组有2头滇南小耳猪死亡。假手术组滇南小耳猪术前心率正常,节律规整,无ST段抬高,术后心率较术前增快;AMI组、心肌缺血再灌注损伤组滇南小耳猪结扎LAD中远段血管后开始出现心率加快,T波高尖、倒置,ST段抬高等ST-T段动态改变;心肌缺血再灌注损伤组滇南小耳猪开放LAD中远段血管后心率仍较快,ST段回落,T波倒置。术后心脏组织病理切片显示,假手术组心肌组织结构完整、心肌纤维条路清晰,细胞排列整齐规则、形态正常;AMI组及心肌缺血再灌注损伤组部分心肌纤维断裂及心肌细胞核溶解消失,细胞空泡样改变,大量炎症细胞浸润。假手术组手术前后cTnⅠ、cMyBP-C水平无明显变化。AMI组滇南小耳猪术后6.0、8.0、10.0、12.0、18.0、24.0 h cTnⅠ水平高于术前,术后1.0、2.0、3.0、4.0、6.0、8.0、10.0、12.0 h cMyBP-C水平高于术前(P<0.05)。AMI组滇南小耳猪cTnⅠ水平术前到术后4.0 h基本稳定,术后6.0 h开始逐渐升高,直至术后24.0 h;AMI组
