Two rice genotypes with different drought tolerance, namely Jin 23B （drought tolerant） and Zhenshan 97B （drought sensitive）, were used to study the antioxidant enzyme activities, soluble sugar and starch contents ...Two rice genotypes with different drought tolerance, namely Jin 23B （drought tolerant） and Zhenshan 97B （drought sensitive）, were used to study the antioxidant enzyme activities, soluble sugar and starch contents in spikelets, pistil water potential and pollen number on a stigma under water-deficit stress at the flowering stage, which were involved in the spikelet sterility. Compared with respective controls, drought stress induced more serious decreases of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） activities and more significant increase of malonaldehyde （MDA） content in spikekets of Zhenshan 97B than in Jin 23B on 9 and 12 days after water stress （DAWS）. The soluble sugar and starch contents increased significantly in spikelets of Jin 23B, but decreased significantly in spikelets of Zhenshan 97B during 9-12 DAWS. The pistil maintained higher water potential in Jin 23B than in Zhenshan 97B during 3-6 DAWS and 9-12 DAWS. In addition, water stress induced more significant decrease in the pollen number on a stigma as well as the percentage of unfilled grains in Zhenshan 97B than in Jin 23B. Thus, it is suggested that water stress induced spikelet sterility by damaging antioxidant enzyme activities, reducing carbohydrate content in spikelets and decreasing pistil water potential at the flowering stage in rice.展开更多
Flooding timing and frequency may have different effects on the growth and carbon metabolism of plants in wetlands. We tested this hypothesis using three marsh plants in Sanjiang Plain, which were growing in different...Flooding timing and frequency may have different effects on the growth and carbon metabolism of plants in wetlands. We tested this hypothesis using three marsh plants in Sanjiang Plain, which were growing in different flooding timing and frequency environments for 24 days. The three marsh plants in different elevations along a water-level gradient, high-elevation species Calamagrostis angustifolia, mid-elevation species Carex limosa and low-elevation species Carex lasiocarpa. Our experiment included 6 kinds of treatments: control, longer flooding timing, medial flooding timing and frequency(2 treatments), and higher flooding frequency(2 treatments). Longer flooding timing and higher flooding frequency treatments led to similar reductions in biomass accumulation and relative growth rate of Calamagrostis angustifolia, whereas growth of Carex limosa was not affected by either flooding timing or frequency. However, biomass accumulation and relative growth rate of Carex lasiocarpa was only reduced by the higher flooding frequency. Starch and soluble sugar contents of Calamagrostis angustifolia were negatively affected by flooding frequency. On Carex limosa, higher flooding frequency resulted in higher starch content, but longer flooding timing led to accumulated starch content in Carex lasiocarpa. Soluble sugar contents of Carex lasiocarpa and Carex li mosa were not affected by either flooding timing or flooding frequency. These results indicated that growth responses of the three marsh plants to flooding timing and frequency was closely related to the different physiology of carbohydrate metabolism.展开更多
基金supported by the National Natural Science Foundation of China(GrantNo.30871473)the project from the China National Rice Research Institute(Grant No.2009RG003-5)the projects from local government of Zhejiang Province,China(Grant Nos.2008C22073,2009C32048 and SN200806)
文摘Two rice genotypes with different drought tolerance, namely Jin 23B （drought tolerant） and Zhenshan 97B （drought sensitive）, were used to study the antioxidant enzyme activities, soluble sugar and starch contents in spikelets, pistil water potential and pollen number on a stigma under water-deficit stress at the flowering stage, which were involved in the spikelet sterility. Compared with respective controls, drought stress induced more serious decreases of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） activities and more significant increase of malonaldehyde （MDA） content in spikekets of Zhenshan 97B than in Jin 23B on 9 and 12 days after water stress （DAWS）. The soluble sugar and starch contents increased significantly in spikelets of Jin 23B, but decreased significantly in spikelets of Zhenshan 97B during 9-12 DAWS. The pistil maintained higher water potential in Jin 23B than in Zhenshan 97B during 3-6 DAWS and 9-12 DAWS. In addition, water stress induced more significant decrease in the pollen number on a stigma as well as the percentage of unfilled grains in Zhenshan 97B than in Jin 23B. Thus, it is suggested that water stress induced spikelet sterility by damaging antioxidant enzyme activities, reducing carbohydrate content in spikelets and decreasing pistil water potential at the flowering stage in rice.
基金Under the auspices of the National Natural Science Foundation of China(31000184)Environmental Public Welfare Sector Special Research Project(2011467032)National Innovation Experiment Program for University Students:Study on the Effects of Water Regime on Primary Succession on Plant Community in Wetlands
文摘Flooding timing and frequency may have different effects on the growth and carbon metabolism of plants in wetlands. We tested this hypothesis using three marsh plants in Sanjiang Plain, which were growing in different flooding timing and frequency environments for 24 days. The three marsh plants in different elevations along a water-level gradient, high-elevation species Calamagrostis angustifolia, mid-elevation species Carex limosa and low-elevation species Carex lasiocarpa. Our experiment included 6 kinds of treatments: control, longer flooding timing, medial flooding timing and frequency(2 treatments), and higher flooding frequency(2 treatments). Longer flooding timing and higher flooding frequency treatments led to similar reductions in biomass accumulation and relative growth rate of Calamagrostis angustifolia, whereas growth of Carex limosa was not affected by either flooding timing or frequency. However, biomass accumulation and relative growth rate of Carex lasiocarpa was only reduced by the higher flooding frequency. Starch and soluble sugar contents of Calamagrostis angustifolia were negatively affected by flooding frequency. On Carex limosa, higher flooding frequency resulted in higher starch content, but longer flooding timing led to accumulated starch content in Carex lasiocarpa. Soluble sugar contents of Carex lasiocarpa and Carex li mosa were not affected by either flooding timing or flooding frequency. These results indicated that growth responses of the three marsh plants to flooding timing and frequency was closely related to the different physiology of carbohydrate metabolism.
