The complex and rapidly progressing nature of sepsis calls for the development of multifunctional and rapid-acting therapeutic agents,instead of single-modal treatments.To address this challenge,a biodegradable,easily...The complex and rapidly progressing nature of sepsis calls for the development of multifunctional and rapid-acting therapeutic agents,instead of single-modal treatments.To address this challenge,a biodegradable,easily synthesized,and antibiotic-free multifunctional nanoparticle has been created for sepsis therapy.The nanoparticle was formed by the electrostatic interaction between two endogenous small molecule-derived polymers,poly(lipoic acid)and poly-lysine,and possessed various functions such as antibacterial activity,adsorption of cell-free DNA,scavenging of reactive oxygen and nitrogen species,providing a comprehensive approach to combating sepsis.Treatment using the cecal ligation and puncture(CLP)model confirmed the therapeutic benefits of the nanoparticles,demonstrating reduced levels of reactive oxygen across multiple organs,diminished levels of M1 proinflammatory macrophages,and elevated levels of M2 anti-inflammatory macrophages post-treatment.These findings emphasized the effectiveness of the nanoparticles in sepsis therapy,and properties of degradation,easy preparation,and swift therapeutic response made them promising for the future clinical applications.展开更多
Breast cancer(BC) is the most common cancer and the second cause of death in women worldwide. Therapeutic options are increasing, but the response to treatments is not always efficient and the risk of recurrence cover...Breast cancer(BC) is the most common cancer and the second cause of death in women worldwide. Therapeutic options are increasing, but the response to treatments is not always efficient and the risk of recurrence covers decades. In this perspective, the need to have a proper follow-up for the therapeutic responses and for anticipating recurrence it is urgent in the clinical setting. Liquid biopsy provides the basic principle for a non-invasive method for the routinely monitoring of BC. However, due to the heterogeneity of tumors during onset and progression, the search for tumor DNA mutations of targeted genes in plasma/serum is a limiting factor. A possible approach overtaking this problem comes from the measurement of cell-free DNA integrity, which is an independent factor from the mutational status and theoretically is representative of all tumors. This review summarizes the state-of-the-art of cell-free DNA integrity researches in BC, the controversies and the future perspective.展开更多
基金supported by the National Key Research and Development Program of China(2021YFB3800900)National Natural Science Foundation of China(51925305,52173115,52073278)Research Foundation for Advanced Talents of Xiamen University of Technology(5010423019,YKJ22052R)。
文摘The complex and rapidly progressing nature of sepsis calls for the development of multifunctional and rapid-acting therapeutic agents,instead of single-modal treatments.To address this challenge,a biodegradable,easily synthesized,and antibiotic-free multifunctional nanoparticle has been created for sepsis therapy.The nanoparticle was formed by the electrostatic interaction between two endogenous small molecule-derived polymers,poly(lipoic acid)and poly-lysine,and possessed various functions such as antibacterial activity,adsorption of cell-free DNA,scavenging of reactive oxygen and nitrogen species,providing a comprehensive approach to combating sepsis.Treatment using the cecal ligation and puncture(CLP)model confirmed the therapeutic benefits of the nanoparticles,demonstrating reduced levels of reactive oxygen across multiple organs,diminished levels of M1 proinflammatory macrophages,and elevated levels of M2 anti-inflammatory macrophages post-treatment.These findings emphasized the effectiveness of the nanoparticles in sepsis therapy,and properties of degradation,easy preparation,and swift therapeutic response made them promising for the future clinical applications.
基金Supported by Ricerca Sanitaria 2015 LILT and Beneficentia Foundation Stiftung,No.BEN2016/16
文摘Breast cancer(BC) is the most common cancer and the second cause of death in women worldwide. Therapeutic options are increasing, but the response to treatments is not always efficient and the risk of recurrence covers decades. In this perspective, the need to have a proper follow-up for the therapeutic responses and for anticipating recurrence it is urgent in the clinical setting. Liquid biopsy provides the basic principle for a non-invasive method for the routinely monitoring of BC. However, due to the heterogeneity of tumors during onset and progression, the search for tumor DNA mutations of targeted genes in plasma/serum is a limiting factor. A possible approach overtaking this problem comes from the measurement of cell-free DNA integrity, which is an independent factor from the mutational status and theoretically is representative of all tumors. This review summarizes the state-of-the-art of cell-free DNA integrity researches in BC, the controversies and the future perspective.
