T cell immunoglobulin- and mucin-domain-containing molecule-3 （Tim-3） has been reported to participate in the pathogenesis of inflammatory diseases. However, whether Tim-3 is involved in hepatitis B virus （HBV） in...T cell immunoglobulin- and mucin-domain-containing molecule-3 （Tim-3） has been reported to participate in the pathogenesis of inflammatory diseases. However, whether Tim-3 is involved in hepatitis B virus （HBV） infection remains unknown. Here, we studied the expression and function of Tim-3 in a hydrodynamics-based mouse model of HBV infection. A significant increase of Tim-3 expression on hepatic T lymphocytes, especially on CD8^＋ T cells, was demonstrated in HBV model mice from day 7 to day 18. After Tim-3 knockdown by specific shRNAs, significantly increased IFN-γ production from hepatic CD8^＋ T cells in HBV model mice was observed. Very interestingly, we found Tim-3 expression on CD8^＋ T cells was higher in HBV model mice with higher serum anti-HBs production. Moreover, Tim-3 knockdown influenced anti-HBs production in vivo. Collectively, our data suggested that Tim-3 might act as a potent regulator of antiviral T-cell responses in HBV infection. Cellular ＆ Molecular Immunology.展开更多
目的探讨外周血淋巴细胞在局部晚期鼻咽癌中的预后价值。方法通过流式细胞术检测88例局部晚期鼻咽癌患者的外周血淋巴细胞亚群比例,比较淋巴细胞亚群在鼻咽癌不同临床特征中的差异性,采用Kaplan-Meier法和Log-rank法单因素分析淋巴细胞...目的探讨外周血淋巴细胞在局部晚期鼻咽癌中的预后价值。方法通过流式细胞术检测88例局部晚期鼻咽癌患者的外周血淋巴细胞亚群比例,比较淋巴细胞亚群在鼻咽癌不同临床特征中的差异性,采用Kaplan-Meier法和Log-rank法单因素分析淋巴细胞亚群比例对预后的影响,采用COX回归模型进行多因素预后相关分析。结果外周血CD3^+T细胞比例在鼻咽癌不同年龄组(P=0.007)、不同N分期组(P=0.043)间差异有统计学意义;外周血NK细胞比例在鼻咽癌不同年龄组(P=0.026)间差异有统计学意义。全组5 a总生存率(OS)为81.78%,5 a无进展生存率(PFS)为76.51%,5 a无局部复发生存率(LRFS)为90.51%,5 a无远处转移生存率(MDFS)为80.09%。低CD8^+T细胞比例组5aLRFS率为84.93%,低于高CD8^+T细胞比例组的100.00%,HR=5.082(1.221～21.150),差异有统计学意义(P=0.026)。年龄(P=0.023)、CD8^+T细胞比例(P=0.013)是局部晚期鼻咽癌5 a OS的影响因素;CD8^+T细胞比例是局部晚期鼻咽癌5 a PFS的独立影响因素(P=0.030)。结论外周血淋巴细胞亚群对局部晚期鼻咽的预后有一定预测价值,尤其是CD8^+T细胞比例是局部晚期鼻咽癌5 a OS和5 a PFS的影响因素。展开更多
基金supported in part by grants from the National Nature Science Foundation of China (No. 30670966)the National Basic Research Program (No. 2009CB521900)+1 种基金the Taishan Scholar Programthe Scientific Foundation of Innovative Research Team in Shandong University.
文摘T cell immunoglobulin- and mucin-domain-containing molecule-3 （Tim-3） has been reported to participate in the pathogenesis of inflammatory diseases. However, whether Tim-3 is involved in hepatitis B virus （HBV） infection remains unknown. Here, we studied the expression and function of Tim-3 in a hydrodynamics-based mouse model of HBV infection. A significant increase of Tim-3 expression on hepatic T lymphocytes, especially on CD8^＋ T cells, was demonstrated in HBV model mice from day 7 to day 18. After Tim-3 knockdown by specific shRNAs, significantly increased IFN-γ production from hepatic CD8^＋ T cells in HBV model mice was observed. Very interestingly, we found Tim-3 expression on CD8^＋ T cells was higher in HBV model mice with higher serum anti-HBs production. Moreover, Tim-3 knockdown influenced anti-HBs production in vivo. Collectively, our data suggested that Tim-3 might act as a potent regulator of antiviral T-cell responses in HBV infection. Cellular ＆ Molecular Immunology.
文摘目的探讨外周血淋巴细胞在局部晚期鼻咽癌中的预后价值。方法通过流式细胞术检测88例局部晚期鼻咽癌患者的外周血淋巴细胞亚群比例,比较淋巴细胞亚群在鼻咽癌不同临床特征中的差异性,采用Kaplan-Meier法和Log-rank法单因素分析淋巴细胞亚群比例对预后的影响,采用COX回归模型进行多因素预后相关分析。结果外周血CD3^+T细胞比例在鼻咽癌不同年龄组(P=0.007)、不同N分期组(P=0.043)间差异有统计学意义;外周血NK细胞比例在鼻咽癌不同年龄组(P=0.026)间差异有统计学意义。全组5 a总生存率(OS)为81.78%,5 a无进展生存率(PFS)为76.51%,5 a无局部复发生存率(LRFS)为90.51%,5 a无远处转移生存率(MDFS)为80.09%。低CD8^+T细胞比例组5aLRFS率为84.93%,低于高CD8^+T细胞比例组的100.00%,HR=5.082(1.221～21.150),差异有统计学意义(P=0.026)。年龄(P=0.023)、CD8^+T细胞比例(P=0.013)是局部晚期鼻咽癌5 a OS的影响因素;CD8^+T细胞比例是局部晚期鼻咽癌5 a PFS的独立影响因素(P=0.030)。结论外周血淋巴细胞亚群对局部晚期鼻咽的预后有一定预测价值,尤其是CD8^+T细胞比例是局部晚期鼻咽癌5 a OS和5 a PFS的影响因素。
