Accumulating evidences have suggested that Treg have an active role in the regulation of immunity to infection. Treg suppress not only autoimmune responses but also other immune responses for instance, during acute in...Accumulating evidences have suggested that Treg have an active role in the regulation of immunity to infection. Treg suppress not only autoimmune responses but also other immune responses for instance, during acute infections, against commensal microbes in inflammatory diseases or during chronic illness. Treg have been shown to limit exacerbated inflammation to avoid collateral tissue damage. Treg are also suggested to provide early protective responses in some viral infections as the permitting timely entry of effector cells in infected tissue. Furthermore, Treg have been shown to contribute to form memory pool after resolution of infection. In this review, we survey and analysis our current knowledge and relative dynamics of Treg in a wide range of infection settings and elaborate the examples in which these cells are of critical importance in conferring tolerance, suppressing pathogenesis, inducing protection and optimizing immunity to eliminate infection.展开更多
目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机...目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机分为电针治疗组、艾灸治疗组、肿瘤对照组、正常对照组。采用H_(22)肿瘤细胞移植性实体瘤模型,电针和艾灸"大椎"治疗后,磁珠分离CD4^+CD25^+Treg细胞,氚标记胸腺嘧啶核苷掺入法观察不同组小鼠CD4^+CD25^+Treg细胞体外增殖能力和针灸血清对CD4^+CD25^+ Treg细胞体外增殖的影响。结果:肿瘤对照组CD4^+CD25^+Treg细胞增殖水平比正常对照组明显增高(P<0.05);电针治疗组和艾灸治疗组CD4^+CD25^+Treg细胞增殖水平与肿瘤对照组比较均明显降低(P<0.01)。电针治疗组和艾灸治疗组1:1、1:8稀释度血清刺激正常小鼠CD4^+CD25^+Treg细胞体外增殖,较正常对照、肿瘤对照组血清显著增高(P<0.05),各组1:16、1:32稀释度血清对Treg细胞作用的差别无统计学意义(P>0.05)。结论:针灸治疗能下调荷瘤小鼠CD4^+CD25^+Treg细胞体外增殖能力。不同浓度针灸血清体外刺激CD4^+CD25^+Treg细胞增殖表现出不一致的效应。展开更多
目的研究薏苡仁酯对胶原诱导性关节炎(collagen induced arthritis,CIA)小鼠外周血中Foxp3+CD4+CD25+调节性T细胞(Treg)的影响,探讨薏苡仁酯类风湿关节炎治疗中的可能机制。方法从25只小鼠随机选取5只为正常组,剩余20只小鼠进行CIA造模...目的研究薏苡仁酯对胶原诱导性关节炎(collagen induced arthritis,CIA)小鼠外周血中Foxp3+CD4+CD25+调节性T细胞(Treg)的影响,探讨薏苡仁酯类风湿关节炎治疗中的可能机制。方法从25只小鼠随机选取5只为正常组,剩余20只小鼠进行CIA造模。造模成功后随机分为造模对照组10只及薏苡仁酯组10只。薏苡仁酯组予薏苡仁酯注射液25 m L/kg,正常组及造模对照组注射生理盐水25 m L/kg,均每天腹腔内注射1次。共用药21天。用药后对各组关节炎进行评分,计算关节炎指数,并应用流式细胞术测定小鼠外周血Foxp3+CD4+CD25+Treg比例。结果与正常组比较,造模对照组关节炎指数明显升高(P<0.01),薏苡仁酯组较造模对照组小鼠关节炎指数明显下降(P<0.01)。与正常组比较,造模对照组Foxp3+CD4+CD25+Treg水平均明显下降(P<0.01),薏苡仁酯组较造模对照组用药后Foxp3+CD4+CD25+Treg水平明显升高,差异有统计学意义(P<0.01)。结论薏苡仁酯能上调CIA小鼠Foxp3+CD4+CD25+Treg比例,可能在CIA的发病中具有一定的免疫调节作用。展开更多
文摘Accumulating evidences have suggested that Treg have an active role in the regulation of immunity to infection. Treg suppress not only autoimmune responses but also other immune responses for instance, during acute infections, against commensal microbes in inflammatory diseases or during chronic illness. Treg have been shown to limit exacerbated inflammation to avoid collateral tissue damage. Treg are also suggested to provide early protective responses in some viral infections as the permitting timely entry of effector cells in infected tissue. Furthermore, Treg have been shown to contribute to form memory pool after resolution of infection. In this review, we survey and analysis our current knowledge and relative dynamics of Treg in a wide range of infection settings and elaborate the examples in which these cells are of critical importance in conferring tolerance, suppressing pathogenesis, inducing protection and optimizing immunity to eliminate infection.
文摘目的:观察针灸治疗对H_(22)荷瘤小鼠CD4^+CD25^+Treg细胞(CD4^+CD25^+regulatory T cells)体外增殖的影响和针灸血清刺激下的CD4^+CD25^+Treg细胞的体外增殖变化,探讨针灸及其血清对荷瘤小鼠免疫调节细胞的干预作用。方法:48只小鼠随机分为电针治疗组、艾灸治疗组、肿瘤对照组、正常对照组。采用H_(22)肿瘤细胞移植性实体瘤模型,电针和艾灸"大椎"治疗后,磁珠分离CD4^+CD25^+Treg细胞,氚标记胸腺嘧啶核苷掺入法观察不同组小鼠CD4^+CD25^+Treg细胞体外增殖能力和针灸血清对CD4^+CD25^+ Treg细胞体外增殖的影响。结果:肿瘤对照组CD4^+CD25^+Treg细胞增殖水平比正常对照组明显增高(P<0.05);电针治疗组和艾灸治疗组CD4^+CD25^+Treg细胞增殖水平与肿瘤对照组比较均明显降低(P<0.01)。电针治疗组和艾灸治疗组1:1、1:8稀释度血清刺激正常小鼠CD4^+CD25^+Treg细胞体外增殖,较正常对照、肿瘤对照组血清显著增高(P<0.05),各组1:16、1:32稀释度血清对Treg细胞作用的差别无统计学意义(P>0.05)。结论:针灸治疗能下调荷瘤小鼠CD4^+CD25^+Treg细胞体外增殖能力。不同浓度针灸血清体外刺激CD4^+CD25^+Treg细胞增殖表现出不一致的效应。
文摘目的研究薏苡仁酯对胶原诱导性关节炎(collagen induced arthritis,CIA)小鼠外周血中Foxp3+CD4+CD25+调节性T细胞(Treg)的影响,探讨薏苡仁酯类风湿关节炎治疗中的可能机制。方法从25只小鼠随机选取5只为正常组,剩余20只小鼠进行CIA造模。造模成功后随机分为造模对照组10只及薏苡仁酯组10只。薏苡仁酯组予薏苡仁酯注射液25 m L/kg,正常组及造模对照组注射生理盐水25 m L/kg,均每天腹腔内注射1次。共用药21天。用药后对各组关节炎进行评分,计算关节炎指数,并应用流式细胞术测定小鼠外周血Foxp3+CD4+CD25+Treg比例。结果与正常组比较,造模对照组关节炎指数明显升高(P<0.01),薏苡仁酯组较造模对照组小鼠关节炎指数明显下降(P<0.01)。与正常组比较,造模对照组Foxp3+CD4+CD25+Treg水平均明显下降(P<0.01),薏苡仁酯组较造模对照组用药后Foxp3+CD4+CD25+Treg水平明显升高,差异有统计学意义(P<0.01)。结论薏苡仁酯能上调CIA小鼠Foxp3+CD4+CD25+Treg比例,可能在CIA的发病中具有一定的免疫调节作用。
