目的建立口服溶液剂洋葱伯克霍尔德菌群检测方法以及产品自身抗污染能力的评价方法。方法采用4种洋葱伯克霍尔德菌模式菌株,分别进行6种口服溶液剂的方法适用性试验,并接种至6种口服液中,持续监测挑战样品中洋葱伯克霍尔德菌的存活情况...目的建立口服溶液剂洋葱伯克霍尔德菌群检测方法以及产品自身抗污染能力的评价方法。方法采用4种洋葱伯克霍尔德菌模式菌株,分别进行6种口服溶液剂的方法适用性试验,并接种至6种口服液中,持续监测挑战样品中洋葱伯克霍尔德菌的存活情况,评价产品的抗污染能力。结果6个品种的口服溶液剂,采用接种至100 mL TSB培养基和1/10 TSB培养基的方法,适用性试验均可检出洋葱伯克霍尔德菌,方法可行,产品对洋葱伯克霍尔德菌群没有明显的抗菌活性;挑战试验结果显示,接种洋葱伯克霍尔德菌放置6个月,部分产品仍可检出存活菌,不同菌株的存活率不同,抗逆性存在差异。结论洋葱伯克霍尔德菌抗逆性较强,生存条件要求不高,常规检查方法易于检出,在口服溶液剂中可长期存活,风险较高,应作为口服溶液剂的常规检测项目,加强生产过程控制。展开更多
Burkholderia cepacia is regarded as a genetically distinct but phenotypically similar bacteria group referring to Burkholderia cepacia complex （Bcc）, which is found not only in clinic but also in rice growing enviro...Burkholderia cepacia is regarded as a genetically distinct but phenotypically similar bacteria group referring to Burkholderia cepacia complex （Bcc）, which is found not only in clinic but also in rice growing environment. It is very important in microbial safety of rice for us to understand the genomovar status of Bcc. Genomovar analysis was performed among 87 Bcc isolates by means of Hae Ⅲ-recA RFLP assays and species-specific PCR tests. Three genomovars were found from the rice rhizosphere including Ⅰ, ⅢB and V, and genomovar V was predominant. Genomovars Ⅰ, ⅢA and ⅢB existed in the clinical samples, and genomovar ⅢA was the most popular. It showed that genomovar composition was different between the Bcc strains from the rice rhizosphere and clinical environment. Simultaneously, the results revealed the genetic diversity of Bcc strains from the rice rhizosphere, and genomovar Ⅲ referred as virulent species in clinic also existed in the rice rhizosphere.展开更多
文摘目的建立口服溶液剂洋葱伯克霍尔德菌群检测方法以及产品自身抗污染能力的评价方法。方法采用4种洋葱伯克霍尔德菌模式菌株,分别进行6种口服溶液剂的方法适用性试验,并接种至6种口服液中,持续监测挑战样品中洋葱伯克霍尔德菌的存活情况,评价产品的抗污染能力。结果6个品种的口服溶液剂,采用接种至100 mL TSB培养基和1/10 TSB培养基的方法,适用性试验均可检出洋葱伯克霍尔德菌,方法可行,产品对洋葱伯克霍尔德菌群没有明显的抗菌活性;挑战试验结果显示,接种洋葱伯克霍尔德菌放置6个月,部分产品仍可检出存活菌,不同菌株的存活率不同,抗逆性存在差异。结论洋葱伯克霍尔德菌抗逆性较强,生存条件要求不高,常规检查方法易于检出,在口服溶液剂中可长期存活,风险较高,应作为口服溶液剂的常规检测项目,加强生产过程控制。
文摘Burkholderia cepacia is regarded as a genetically distinct but phenotypically similar bacteria group referring to Burkholderia cepacia complex （Bcc）, which is found not only in clinic but also in rice growing environment. It is very important in microbial safety of rice for us to understand the genomovar status of Bcc. Genomovar analysis was performed among 87 Bcc isolates by means of Hae Ⅲ-recA RFLP assays and species-specific PCR tests. Three genomovars were found from the rice rhizosphere including Ⅰ, ⅢB and V, and genomovar V was predominant. Genomovars Ⅰ, ⅢA and ⅢB existed in the clinical samples, and genomovar ⅢA was the most popular. It showed that genomovar composition was different between the Bcc strains from the rice rhizosphere and clinical environment. Simultaneously, the results revealed the genetic diversity of Bcc strains from the rice rhizosphere, and genomovar Ⅲ referred as virulent species in clinic also existed in the rice rhizosphere.
