AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in se...AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in serum and PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-alpha therapy using a nested RT/PCR technique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma. RESULTS: In the IFN-alpha responding patients,HCV-RNA disappeared first from total RNA preparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNA reappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNA concentration in serum was performed before and after transition from detectable to non detectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMC preparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMC from healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration dependent PCR positivity for HCV-RNA in reisolated PBMC. CONCLUSION: The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMC preparations of chronically infected patients.展开更多
AIM: To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou. METHODS: RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nu...AIM: To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou. METHODS: RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed. RESULTS: Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively. CONCLUSION: The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.展开更多
目的探讨乌拉地尔联合硝酸甘油治疗高血压急症的临床疗效。方法选取2013年11月—2015年11月在延安市人民医院进行治疗的高血压急症患者60例,根据治疗方案的差别分为对照组和治疗组,每组各30例。对照组静脉滴注硝酸甘油注射液,10 mg加入...目的探讨乌拉地尔联合硝酸甘油治疗高血压急症的临床疗效。方法选取2013年11月—2015年11月在延安市人民医院进行治疗的高血压急症患者60例,根据治疗方案的差别分为对照组和治疗组,每组各30例。对照组静脉滴注硝酸甘油注射液,10 mg加入到生理盐水250 m L中,滴速10-40μg/min,且根据血压调整滴速。治疗组在对照组的基础上静脉微泵注射盐酸乌拉地尔注射液,50 mg溶于生理盐水50 m L中,滴速100-400μg/min,根据血压调整速度。血压和临床表现稳定后,两组患者用药均逐渐减量。观察两组的临床疗效,同时比较两组患者治疗前后的血压和心率变化及不良反应发生情况。结果治疗后,对照组和治疗组的总有效率分别为80.00%、95.00%,两组比较差异有统计学意义（P〈0.05）。治疗5、30、60、120 min后,两组患者的SBP、DBP和HR均较同组治疗前显著改善,同组治疗前后差异具有统计学意义（P〈0.05）;且治疗5、30 min后,治疗组患者的SBP、DBP和HR改善程度明显优于同期对照组,两组比较差异具有统计学意义（P〈0.05）。结论乌拉地尔联合硝酸甘油治疗高血压急症效果显著,安全性高、不良反应低,具有一定的临床推广应用价值。展开更多
基金Supported by a grant of DFG (SFB 402 Teilprojekt C1 (Mihm))by a grant of Hoffmann La Roche (Grenzach-Wyhden, Germany)Part of the data has been presented as poster at the 1999 EASL-meeting in Neaples
文摘AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in serum and PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-alpha therapy using a nested RT/PCR technique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma. RESULTS: In the IFN-alpha responding patients,HCV-RNA disappeared first from total RNA preparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNA reappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNA concentration in serum was performed before and after transition from detectable to non detectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMC preparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMC from healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration dependent PCR positivity for HCV-RNA in reisolated PBMC. CONCLUSION: The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMC preparations of chronically infected patients.
文摘目的分析小剂量拉贝洛尔片联合硝苯地平治疗原发性高血压(EH)1级孕妇的临床效果。方法将150例EH 1级孕妇随机分为联合组和硝苯地平组各75例,分别给予小剂量拉贝洛尔+硝苯地平、硝苯地平治疗。监测治疗前及治疗后2、4周孕妇的血压及母胎心率,评价其治疗效果,并观察母婴结局及药物不良反应发生情况。结果治疗后2、4周,两组孕妇的血压均较前降低,且联合组的血压低于硝苯地平组;硝苯地平组孕妇与胎儿心率均高于治疗前,而联合组的孕妇与胎儿心率低于治疗前水平及硝苯地平组(均P<0.05)。联合组治疗后2周的显效率及总有效率高于硝苯地平组(均P<0.05),且治疗后4周的显效率达100%,亦高于硝苯地平组(均P<0.05)。联合组无子痫前期发生,孕妇心脏期前收缩消失;硝苯地平组出现低血压1例,子痫前期、胎盘早剥各2例。联合组新生儿出生胎龄、体重、出生后1 min Apgar评分均大于硝苯地平组,而不良反应发生率低于硝苯地平组(均P<0.05)。结论与单纯应用硝苯地平比较,小剂量拉贝洛尔联合硝苯地平可更好更快地控制EH 1级孕妇的血压,适当减慢孕妇与胎儿心率,减少药物不良反应,改善母婴结局。
文摘AIM: To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou. METHODS: RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed. RESULTS: Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively. CONCLUSION: The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.
文摘目的探讨乌拉地尔联合硝酸甘油治疗高血压急症的临床疗效。方法选取2013年11月—2015年11月在延安市人民医院进行治疗的高血压急症患者60例,根据治疗方案的差别分为对照组和治疗组,每组各30例。对照组静脉滴注硝酸甘油注射液,10 mg加入到生理盐水250 m L中,滴速10-40μg/min,且根据血压调整滴速。治疗组在对照组的基础上静脉微泵注射盐酸乌拉地尔注射液,50 mg溶于生理盐水50 m L中,滴速100-400μg/min,根据血压调整速度。血压和临床表现稳定后,两组患者用药均逐渐减量。观察两组的临床疗效,同时比较两组患者治疗前后的血压和心率变化及不良反应发生情况。结果治疗后,对照组和治疗组的总有效率分别为80.00%、95.00%,两组比较差异有统计学意义（P〈0.05）。治疗5、30、60、120 min后,两组患者的SBP、DBP和HR均较同组治疗前显著改善,同组治疗前后差异具有统计学意义（P〈0.05）;且治疗5、30 min后,治疗组患者的SBP、DBP和HR改善程度明显优于同期对照组,两组比较差异具有统计学意义（P〈0.05）。结论乌拉地尔联合硝酸甘油治疗高血压急症效果显著,安全性高、不良反应低,具有一定的临床推广应用价值。
