目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘...目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘>5 cm),q PCR检测膀胱癌及癌旁组织miR-372-3p表达。采用脂质体转染法将miR-372-3p mimics或者miRNC转入膀胱癌5637和T24细胞。用q PCR和Western blotting检测转染miR-372-3p mimics和miR-NC膀胱癌细胞miR-372-3p和ATAD2 m RNA和蛋白E-cadherin、N-cadherin蛋白的表达,流式细胞术检测细胞周期分布,MTT法和集落形成实验检测细胞增殖和集落形成能力,划痕实验和Transwell侵袭实验检测细胞的迁移和侵袭能力。结果:膀胱癌组织miR-372-3p m RNA的表达显著低于癌旁组织(0.65±0.56 vs 1.76±0.34,P<0.01)。和转染miR-NC膀胱癌细胞相比,转染miR-372-3p mimics膀胱癌细胞的miR-372-3p m RNA表达显著增加,ATAD2 m RNA和蛋白的表达显著降低,E-cadherin蛋白表达上调,N-cadherin蛋白表达下调,细胞周期明显阻滞,细胞集落形成和增殖能力显著降低,细胞迁移数和侵袭数减少。结论:miR-372-3p的低表达可能与膀胱癌的发生发展有关,其通过靶向调控ATAD2抑制膀胱癌细胞的增殖、迁移和侵袭能力,可能成为膀胱癌生物治疗的新靶标。展开更多
目的探讨膀胱癌组织NLRP3的表达水平,分析其与膀胱癌临床特征的相关性;体外研究膀胱癌细胞系NLRP3的表达水平及NLRP3对膀胱癌细胞增殖、迁移和侵袭能力的影响。方法收集行手术切除的膀胱癌组织标本31例和癌旁组织标本8例,应用实时荧光定...目的探讨膀胱癌组织NLRP3的表达水平,分析其与膀胱癌临床特征的相关性;体外研究膀胱癌细胞系NLRP3的表达水平及NLRP3对膀胱癌细胞增殖、迁移和侵袭能力的影响。方法收集行手术切除的膀胱癌组织标本31例和癌旁组织标本8例,应用实时荧光定量PCR(q PCR)和Western blot分别检测NLRP3的表达水平,分析患者临床资料与NLRP3基因表达的相关性。体外应用膀胱癌细胞(T24、5637和BIU-87)研究NLRP3对膀胱癌细胞增殖、迁移和侵袭能力的作用。结果与癌旁组织相比,NLRP3 m RNA和蛋白在膀胱癌组织中表达显著上调。NLRP3 m RNA的表达与病理分期(p T)、淋巴结转移(p N)和WHO肿瘤分级相关(P<0.01)。肿瘤细胞系中NLRP3表达显著高于人正常膀胱上皮细胞,其中5637及T24细胞中NLRP3表达显著升高(P<0.01)。体外沉默NLRP3显著抑制5637及T24的细胞增殖、迁移和侵袭。结论 NLRP3表达与膀胱癌的恶性特征及癌细胞增殖、迁移和侵袭能力密切相关,有望成为膀胱癌的潜在治疗靶点。展开更多
Background: A major cause of cancer death worldwide is bladder cancer, which is the most common malignant tumor of the urinary tract. PAEP is a member of the kernel lipocalin superfamily whose members share relatively...Background: A major cause of cancer death worldwide is bladder cancer, which is the most common malignant tumor of the urinary tract. PAEP is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The purpose of this study was to clarify the correlation between PAEP expression level and bladder cancer. Methods: In the TCGA database, we obtained clinical and RNA sequencing data of 431 BLCA patients, including 412 BLCA tissues and 19 normal bladder tissues in the study. Analyses of bioinformatics were conducted in this study to determine the role of PAEP in bladder cancer. A quantitative real-time PCR method was used to quantitate the gene expression profile. Additionally, the effect of PAEP on tumor immune infiltration and prognosis was analyzed. Results: PAEP was a poor prognostic biomarker of bladder cancer because it was significantly upregulated. bladder cancer patients with higher PAEP expression had poor outcomes. An AUC of 0.780 was calculated from the area under the ROC curve. PAEP was associated with T stage, pathologic stage, Histologic grade and Subtype of bladder cancer patients, and served as an independent predictor of overall survival in bladder cancer patients. Functional enrichment analysis revealed PAEP was obviously enriched in pathways connected with carcinogenesis and immunosuppression. The expression of PAEP was significantly associated with tumor immune cells and immune checkpoints according to ssGSEA and Spearman correlation analysis. Conclusions: In this study, we screened and detected a mRNA, PAEP is a prognostic and immune-related biomarker in BLCA, which may contribute to the early diagnosis and treatment of BLCA.展开更多
文摘目的:检测miR-372-3p在膀胱癌组织和转染miR-372-3p mimics膀胱癌细胞中的表达,研究miR-372-3p对膀胱癌5637和T24细胞增殖、迁移与侵袭的影响。方法:采集2016年3月至2017年1月武汉中心医院收治的6例膀胱癌患者癌及癌旁组织(距离肿瘤边缘>5 cm),q PCR检测膀胱癌及癌旁组织miR-372-3p表达。采用脂质体转染法将miR-372-3p mimics或者miRNC转入膀胱癌5637和T24细胞。用q PCR和Western blotting检测转染miR-372-3p mimics和miR-NC膀胱癌细胞miR-372-3p和ATAD2 m RNA和蛋白E-cadherin、N-cadherin蛋白的表达,流式细胞术检测细胞周期分布,MTT法和集落形成实验检测细胞增殖和集落形成能力,划痕实验和Transwell侵袭实验检测细胞的迁移和侵袭能力。结果:膀胱癌组织miR-372-3p m RNA的表达显著低于癌旁组织(0.65±0.56 vs 1.76±0.34,P<0.01)。和转染miR-NC膀胱癌细胞相比,转染miR-372-3p mimics膀胱癌细胞的miR-372-3p m RNA表达显著增加,ATAD2 m RNA和蛋白的表达显著降低,E-cadherin蛋白表达上调,N-cadherin蛋白表达下调,细胞周期明显阻滞,细胞集落形成和增殖能力显著降低,细胞迁移数和侵袭数减少。结论:miR-372-3p的低表达可能与膀胱癌的发生发展有关,其通过靶向调控ATAD2抑制膀胱癌细胞的增殖、迁移和侵袭能力,可能成为膀胱癌生物治疗的新靶标。
文摘目的探讨膀胱癌组织NLRP3的表达水平,分析其与膀胱癌临床特征的相关性;体外研究膀胱癌细胞系NLRP3的表达水平及NLRP3对膀胱癌细胞增殖、迁移和侵袭能力的影响。方法收集行手术切除的膀胱癌组织标本31例和癌旁组织标本8例,应用实时荧光定量PCR(q PCR)和Western blot分别检测NLRP3的表达水平,分析患者临床资料与NLRP3基因表达的相关性。体外应用膀胱癌细胞(T24、5637和BIU-87)研究NLRP3对膀胱癌细胞增殖、迁移和侵袭能力的作用。结果与癌旁组织相比,NLRP3 m RNA和蛋白在膀胱癌组织中表达显著上调。NLRP3 m RNA的表达与病理分期(p T)、淋巴结转移(p N)和WHO肿瘤分级相关(P<0.01)。肿瘤细胞系中NLRP3表达显著高于人正常膀胱上皮细胞,其中5637及T24细胞中NLRP3表达显著升高(P<0.01)。体外沉默NLRP3显著抑制5637及T24的细胞增殖、迁移和侵袭。结论 NLRP3表达与膀胱癌的恶性特征及癌细胞增殖、迁移和侵袭能力密切相关,有望成为膀胱癌的潜在治疗靶点。
文摘Background: A major cause of cancer death worldwide is bladder cancer, which is the most common malignant tumor of the urinary tract. PAEP is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The purpose of this study was to clarify the correlation between PAEP expression level and bladder cancer. Methods: In the TCGA database, we obtained clinical and RNA sequencing data of 431 BLCA patients, including 412 BLCA tissues and 19 normal bladder tissues in the study. Analyses of bioinformatics were conducted in this study to determine the role of PAEP in bladder cancer. A quantitative real-time PCR method was used to quantitate the gene expression profile. Additionally, the effect of PAEP on tumor immune infiltration and prognosis was analyzed. Results: PAEP was a poor prognostic biomarker of bladder cancer because it was significantly upregulated. bladder cancer patients with higher PAEP expression had poor outcomes. An AUC of 0.780 was calculated from the area under the ROC curve. PAEP was associated with T stage, pathologic stage, Histologic grade and Subtype of bladder cancer patients, and served as an independent predictor of overall survival in bladder cancer patients. Functional enrichment analysis revealed PAEP was obviously enriched in pathways connected with carcinogenesis and immunosuppression. The expression of PAEP was significantly associated with tumor immune cells and immune checkpoints according to ssGSEA and Spearman correlation analysis. Conclusions: In this study, we screened and detected a mRNA, PAEP is a prognostic and immune-related biomarker in BLCA, which may contribute to the early diagnosis and treatment of BLCA.
