Objective To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand （BOD） which is developed by our research group based on suspended immobilized microbial cell s...Objective To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand （BOD） which is developed by our research group based on suspended immobilized microbial cell system in a completely mixed determining chamber as a substitute of the traditional membrane system. Methods Activated sludge was immobilized by PVA gel and used as a bio-sensing element. The novel biosensor was used to measure the short time BOD value and the conventional cultivation method was used for BOD5 measurement. Results A linear relationship was observed for the difference between the current and the concentration of glucose-glutamic acid （GGA） solution below 200 mg/L with a correlation coefficient of 0.995. The optimal response of the sensor was obtained at pH 7.0 and 30℃. The sensor response was within 15 min and was reproducible within ±5% of the mean in a series of eight samples containing 75 mg/L BOD using standard GGA solution. The novel sensor response was found to be fairly constant over a period of 30 days, with ±5% fluctuations. Conclusion A relatively good agreement is found between BOD estimated by the novel BOD biosensor and that determined by the conventional 5-day BOD method. This novel BOD biosensor has good sensitivity, stability and reproducibility.展开更多
生化需氧量(BOD)是衡量水体有机污染程度的重要指标之一。传统的五天培养法测定耗时费力、实效性差。BOD 微生物传感器以其方便、快速、准确等优点得到了迅速发展。该文综述了 BOD 微生物传感器的原理、构成、最新研究进展并对菌种和固...生化需氧量(BOD)是衡量水体有机污染程度的重要指标之一。传统的五天培养法测定耗时费力、实效性差。BOD 微生物传感器以其方便、快速、准确等优点得到了迅速发展。该文综述了 BOD 微生物传感器的原理、构成、最新研究进展并对菌种和固定化方法做了详细比较说明。展开更多
基金The project was supported by the National Natural Science Foundation of China (Grant No. 50327802).
文摘Objective To investigate the function of a novel biosensor used for the rapid determination of biochemical oxygen demand （BOD） which is developed by our research group based on suspended immobilized microbial cell system in a completely mixed determining chamber as a substitute of the traditional membrane system. Methods Activated sludge was immobilized by PVA gel and used as a bio-sensing element. The novel biosensor was used to measure the short time BOD value and the conventional cultivation method was used for BOD5 measurement. Results A linear relationship was observed for the difference between the current and the concentration of glucose-glutamic acid （GGA） solution below 200 mg/L with a correlation coefficient of 0.995. The optimal response of the sensor was obtained at pH 7.0 and 30℃. The sensor response was within 15 min and was reproducible within ±5% of the mean in a series of eight samples containing 75 mg/L BOD using standard GGA solution. The novel sensor response was found to be fairly constant over a period of 30 days, with ±5% fluctuations. Conclusion A relatively good agreement is found between BOD estimated by the novel BOD biosensor and that determined by the conventional 5-day BOD method. This novel BOD biosensor has good sensitivity, stability and reproducibility.
