目的 探讨 Apo E基因多态性与血管性痴呆 ( Va D)的相互关系。方法 应用聚合酶链反应 -限制性片段长度多态性 ( PCR-RFLP)技术 ,检测脑卒中后 Va D组与未发生 Va D的对照组患者的 Apo E基因型分布及出现频率。结果 Apo E基因的ε4 ...目的 探讨 Apo E基因多态性与血管性痴呆 ( Va D)的相互关系。方法 应用聚合酶链反应 -限制性片段长度多态性 ( PCR-RFLP)技术 ,检测脑卒中后 Va D组与未发生 Va D的对照组患者的 Apo E基因型分布及出现频率。结果 Apo E基因的ε4 / 4基因型 ,在 Va D组的出现频率明显高于未发生 Va D的对照组 ( P<0 .0 5 ) ,其余各基因型的出现频率 ,两组间差异无显著性 ( P>0 .0 5 )。结论 Apo E基因的 ε4 / 4基因型与 Va D的发生密切相关 ;等位基因ε4可能是 Va D的一种遗传易感性因子。展开更多
Background: Psoriasis is associated with abnormal plasma lipid metabolism and a high frequency of cardiovascular events. Increased lipid levels are also seen in patients with psoriasis treated with acitretin. Apolipop...Background: Psoriasis is associated with abnormal plasma lipid metabolism and a high frequency of cardiovascular events. Increased lipid levels are also seen in patients with psoriasis treated with acitretin. Apolipoprotein E (ApoE) variants have been linked to hypertriglyceridaemia and hypercholesterolaemia in normal individuals. Two coding single nucleotide polymorphisms at +3937 and +4075 define the three common ApoE alleles e2, e3 and e4. Objectives: To test the hypothesis that particular ApoE polymorphism(s) are associated with psoriasis and that specific ApoE allelic variant(s) may be a marker for predicting disease response to acitretin. Methods: DNA was genotyped for ApoE polymorphisms using a radioactive hybridization technique in cohorts of patients with psoriasis, including patients with chronic plaque psoriasis (CPP, n = 212), guttate psoriasis (GP, n = 94), palmoplantar pustulosis (PPP, n = 101), controls (n = 137), acitretin responders (n =106) and acitretin nonresponders (n = 84). Results: The frequency of the e4 allele (+3937C/+4075C) was significantly higher in patients with CPP and GP than in controls (P = 0.008 and P = 0.02, respectively). There was no significant difference in allele frequencies between patients with PPP and controls. Allelic distribution was similar in acitretin responders and nonresponders. Conclusions: These data demonstrate an association between the Apo e4 allele and CPP and GP, suggesting a possible pathogenic role for ApoE in psoriasis. Our results do not support a link between disease response to acitretin and the e2, e3 or e4 allelic variants of ApoE.展开更多
目的探讨轻度认知损害(M C I)患者认知功能改变以及认知功能改变和载脂蛋白E(A poE)ε4等位基因的关系。方法应用神经心理学方法评估患者的认知功能状况,应用分子生物学的方法进行APOE的基因型检测。结果①除记忆力显著下降外,M C I组...目的探讨轻度认知损害(M C I)患者认知功能改变以及认知功能改变和载脂蛋白E(A poE)ε4等位基因的关系。方法应用神经心理学方法评估患者的认知功能状况,应用分子生物学的方法进行APOE的基因型检测。结果①除记忆力显著下降外,M C I组定向力、语言能力、执行等能力也呈下降趋势。②M C I组ε4等位基因出现的频率远高于正常老年组(P<0.01)。③A poEε4的携带者的认知功能的总评分显著低于非A poEε4携带者(P<0.001)。除记忆力表现出显著下降外,A poEε4携带组的语言能力、执行也呈下降趋势(P<0.05)。结论轻度认知功能损害患者的认知功能出现改变,且A poEε4等位基因出现的频率显著高于正常老年组。A poEε4等位基因与老年人认知功能的下降相关。展开更多
文摘目的 探讨 Apo E基因多态性与血管性痴呆 ( Va D)的相互关系。方法 应用聚合酶链反应 -限制性片段长度多态性 ( PCR-RFLP)技术 ,检测脑卒中后 Va D组与未发生 Va D的对照组患者的 Apo E基因型分布及出现频率。结果 Apo E基因的ε4 / 4基因型 ,在 Va D组的出现频率明显高于未发生 Va D的对照组 ( P<0 .0 5 ) ,其余各基因型的出现频率 ,两组间差异无显著性 ( P>0 .0 5 )。结论 Apo E基因的 ε4 / 4基因型与 Va D的发生密切相关 ;等位基因ε4可能是 Va D的一种遗传易感性因子。
文摘Background: Psoriasis is associated with abnormal plasma lipid metabolism and a high frequency of cardiovascular events. Increased lipid levels are also seen in patients with psoriasis treated with acitretin. Apolipoprotein E (ApoE) variants have been linked to hypertriglyceridaemia and hypercholesterolaemia in normal individuals. Two coding single nucleotide polymorphisms at +3937 and +4075 define the three common ApoE alleles e2, e3 and e4. Objectives: To test the hypothesis that particular ApoE polymorphism(s) are associated with psoriasis and that specific ApoE allelic variant(s) may be a marker for predicting disease response to acitretin. Methods: DNA was genotyped for ApoE polymorphisms using a radioactive hybridization technique in cohorts of patients with psoriasis, including patients with chronic plaque psoriasis (CPP, n = 212), guttate psoriasis (GP, n = 94), palmoplantar pustulosis (PPP, n = 101), controls (n = 137), acitretin responders (n =106) and acitretin nonresponders (n = 84). Results: The frequency of the e4 allele (+3937C/+4075C) was significantly higher in patients with CPP and GP than in controls (P = 0.008 and P = 0.02, respectively). There was no significant difference in allele frequencies between patients with PPP and controls. Allelic distribution was similar in acitretin responders and nonresponders. Conclusions: These data demonstrate an association between the Apo e4 allele and CPP and GP, suggesting a possible pathogenic role for ApoE in psoriasis. Our results do not support a link between disease response to acitretin and the e2, e3 or e4 allelic variants of ApoE.
文摘目的探讨轻度认知损害(M C I)患者认知功能改变以及认知功能改变和载脂蛋白E(A poE)ε4等位基因的关系。方法应用神经心理学方法评估患者的认知功能状况,应用分子生物学的方法进行APOE的基因型检测。结果①除记忆力显著下降外,M C I组定向力、语言能力、执行等能力也呈下降趋势。②M C I组ε4等位基因出现的频率远高于正常老年组(P<0.01)。③A poEε4的携带者的认知功能的总评分显著低于非A poEε4携带者(P<0.001)。除记忆力表现出显著下降外,A poEε4携带组的语言能力、执行也呈下降趋势(P<0.05)。结论轻度认知功能损害患者的认知功能出现改变,且A poEε4等位基因出现的频率显著高于正常老年组。A poEε4等位基因与老年人认知功能的下降相关。
