目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌...目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌株对甲硝唑及克拉霉素的最低抑菌浓度(MIC)。对所有入选患者采用兰索拉唑、甲硝唑和克拉霉素的三联7d 疗法进行 Hp 根除治疗。结果共有157例患者进入本研究,并完成了治疗。Hp 对甲硝唑和克拉霉素的耐药率分别为:45.9％(72/157)和18.5％(29/157)。157例接受治疗患者的 Hp 根除率为61.8％(97/157),Hp 根除率在不同性别、年龄及不同临床疾病患者之间差异均无统计学意义(均 P>0.05);甲硝唑敏感菌株和耐药菌株的根除率分别为89.4％(76/85)和29.2％(21/72)(P<0.01)。克拉霉素敏感菌株和耐药菌株的根除率分别为72.7％(93/128)和16.0％(4/29)(P<0.01)。结论 Hp 对抗生素耐药是导致Hp 根除治疗失败的主要原因。展开更多
Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical ...Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical antibiotic-resistant H. pylori strains were isolated and incubated in liquid medium containing Jinghua Weikang Capsule or Chenopodium ambrosioides L. By means of time-kill curve method, the average colony counts and bactericidal rate were calculated at time points of 0, 4, 8 and 24 h after the incubation and the time-kill curves were charted. Results： Both Jinghua Weikang Capsule and Chenopodium ambrosioides L. at a concentration of 0.64 g/L showed obvious bactericidal effect against antibiotic-resistant H. pylori after 4 h of incubation. Conclusion： Jinghua Weikang Capsule and Chenopodium ambrosioides L. are considered to be active against antibiotic-resistant H. pylori in vitro.展开更多
With the development and rising of antimicrobial resistance,rapid and effective killings of bacteria are urgently needed,especially for antibiotic-resistant bacteria and bacterial biofilms that are usually hard to be ...With the development and rising of antimicrobial resistance,rapid and effective killings of bacteria are urgently needed,especially for antibiotic-resistant bacteria and bacterial biofilms that are usually hard to be treated with conventional antibiotics.Here,a rapid and broad-spectrum antibacterial strategy is demonstrated through photothermal ablation with MXene and light.Ti3C2 MXenes,when combined with 808 nm light,show significant antibacterial effects in just 20 min.The antibacterial strategy is effective to 15 bacterial species tested,including methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococci(VRE).In addition,the rapid antibacterial strategy works for MRSA biofilms,by damaging the structures as well as killing bacteria in biofilms.Furthermore,the investigation of the antibacterial mechanisms shows that Ti3C2 with light kills bacteria mainly physically through inserting/contact and photothermal effect.This work broadens the potential applications of MXene and provides a way to eradicate bacteria and biofilms physically,without the likelihood of resistance development.展开更多
文摘目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌株对甲硝唑及克拉霉素的最低抑菌浓度(MIC)。对所有入选患者采用兰索拉唑、甲硝唑和克拉霉素的三联7d 疗法进行 Hp 根除治疗。结果共有157例患者进入本研究,并完成了治疗。Hp 对甲硝唑和克拉霉素的耐药率分别为:45.9％(72/157)和18.5％(29/157)。157例接受治疗患者的 Hp 根除率为61.8％(97/157),Hp 根除率在不同性别、年龄及不同临床疾病患者之间差异均无统计学意义(均 P>0.05);甲硝唑敏感菌株和耐药菌株的根除率分别为89.4％(76/85)和29.2％(21/72)(P<0.01)。克拉霉素敏感菌株和耐药菌株的根除率分别为72.7％(93/128)和16.0％(4/29)(P<0.01)。结论 Hp 对抗生素耐药是导致Hp 根除治疗失败的主要原因。
基金Supported by Capital Medical Development and Research Foundation(No.SF-2007-Ⅲ-01)National Natural Science Foundation of China(No.8107295)
文摘Objective： To investigate the bactericidal effects of Jinghua Weikang Capsule （荆花胃康胶丸） and its major component Chenopodium ambrosioides L. on antibiotic-resistant Helicobacter pylori. Methods： Four clinical antibiotic-resistant H. pylori strains were isolated and incubated in liquid medium containing Jinghua Weikang Capsule or Chenopodium ambrosioides L. By means of time-kill curve method, the average colony counts and bactericidal rate were calculated at time points of 0, 4, 8 and 24 h after the incubation and the time-kill curves were charted. Results： Both Jinghua Weikang Capsule and Chenopodium ambrosioides L. at a concentration of 0.64 g/L showed obvious bactericidal effect against antibiotic-resistant H. pylori after 4 h of incubation. Conclusion： Jinghua Weikang Capsule and Chenopodium ambrosioides L. are considered to be active against antibiotic-resistant H. pylori in vitro.
基金the National Natural Science Foundation of China(81901790 and 21803006)the Natural Science Foundation of Beijing(7204274)+1 种基金the Fundamental Research Funds for the Central Universitiesthe Interdisciplinary Medicine Seed Fund of Peking University(BMU2017MX015)。
文摘With the development and rising of antimicrobial resistance,rapid and effective killings of bacteria are urgently needed,especially for antibiotic-resistant bacteria and bacterial biofilms that are usually hard to be treated with conventional antibiotics.Here,a rapid and broad-spectrum antibacterial strategy is demonstrated through photothermal ablation with MXene and light.Ti3C2 MXenes,when combined with 808 nm light,show significant antibacterial effects in just 20 min.The antibacterial strategy is effective to 15 bacterial species tested,including methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococci(VRE).In addition,the rapid antibacterial strategy works for MRSA biofilms,by damaging the structures as well as killing bacteria in biofilms.Furthermore,the investigation of the antibacterial mechanisms shows that Ti3C2 with light kills bacteria mainly physically through inserting/contact and photothermal effect.This work broadens the potential applications of MXene and provides a way to eradicate bacteria and biofilms physically,without the likelihood of resistance development.
