摘要
With the development and rising of antimicrobial resistance,rapid and effective killings of bacteria are urgently needed,especially for antibiotic-resistant bacteria and bacterial biofilms that are usually hard to be treated with conventional antibiotics.Here,a rapid and broad-spectrum antibacterial strategy is demonstrated through photothermal ablation with MXene and light.Ti3C2 MXenes,when combined with 808 nm light,show significant antibacterial effects in just 20 min.The antibacterial strategy is effective to 15 bacterial species tested,including methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococci(VRE).In addition,the rapid antibacterial strategy works for MRSA biofilms,by damaging the structures as well as killing bacteria in biofilms.Furthermore,the investigation of the antibacterial mechanisms shows that Ti3C2 with light kills bacteria mainly physically through inserting/contact and photothermal effect.This work broadens the potential applications of MXene and provides a way to eradicate bacteria and biofilms physically,without the likelihood of resistance development.
随着细菌耐药性的不断出现和加重,针对耐药菌感染和菌膜这类难以用传统抗生素治疗的临床医疗问题,需要发展新型高效快速的杀菌方法.本文采用新型二维材料MXene与近红外激光相结合,实现了在20 min内对细菌以及菌膜的快速高效杀除.为了测试该方案的广谱抗菌性,我们对包括耐药性的耐甲氧西林金黄色葡萄球菌(MRSA)和耐万古霉素肠球菌(VRE)等多种耐药菌进行了快速灭菌实验.我们发现二碳化三钛类的MXene与808 nm激光的组合,对所测试的15种细菌均展现出显著的抗菌特性.并且该杀菌方案可通过破坏菌膜结构杀灭深层细菌,快速清除由MRSA所形成的菌膜.此外,对抗菌机理的研究显示,MXene和近红外激光主要是通过物理性的MXene插入及接触和光热效应杀死细菌,从而可以显著地降低细菌耐药性的产生.本工作提出了一种物理性清除多种耐药菌和菌膜的方案,并扩展了新型二维材料MXene在生物医学领域和临床上的潜在应用范围.
作者
Fan Wu
Huiling Zheng
Wenzhao Wang
Qiong Wu
Qi Zhang
Jiayu Guo
Bangzheng Pu
Xinyuan Shi
Jiebo Li
Xiangmei Chen
Weili Hong
吴凡;郑惠玲;王文钊;吴琼;张琦;郭佳钰;普邦政;史芯源;李介博;陈香梅;洪维礼(Institute of Medical Photonics,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing,100191,China;Department of Microbiology and Infectious Disease Center,School of Basic Medical Sciences,Peking University Health Science Center,Beijing,100191,China;State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of the Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China)
基金
the National Natural Science Foundation of China(81901790 and 21803006)
the Natural Science Foundation of Beijing(7204274)
the Fundamental Research Funds for the Central Universities
the Interdisciplinary Medicine Seed Fund of Peking University(BMU2017MX015)。
