Rheumatoid arthritis （RA） is the most common inflammatory arthritis and a major cause of disability. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to se...Rheumatoid arthritis （RA） is the most common inflammatory arthritis and a major cause of disability. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to severe adverse effects or ineffectiveness. The Guge Fengtong formula （GGFT）, containing the standardized extracts of Dioscoreae Nipponicae Rhizoma, Spatholobi Caulis, and Zingiberis Rhizoma, has long been used for RA treatment in China. However, the detailed anti-inflammatory and anti-arthritic activity of GGFT has not been reported so far. In the present work, we aimed to evaluate the anti-inflammatory and anti-arthritic effects of GGFT using three in vivo animal models and tried to uncover the underlying mechanism of action in RAW 264.7 macrophages. The obtained results indicated that GGFT significantly attenuated ear edema, decreased carrageenan-induced paw edema, reduced the ar- thritis score, and reversed the weight loss of the complete Freund＇s adjuvant （CFA）-injected rats. Additionally, decrease in synovial inflammatory infiltration and synovial lining hyperplasia in the joints and decline of inflammatory factors （TNF-α and IL-1β） in the serum were observed in the GGFT-treated rats. In lipopolysaccharide-activated RAW264.7 macrophages, GGFT reduced the produc- tion of NO, PGE2, and IL-6 and inhibited the expression of iNOS, COX-2, and NF-kB. Our results demonstrated that GGFT possessed considerable anti-inflammatory activity and had potential therapeutic effects on adjuvant induced arthritis in rats, providing experi- mental evidences for its application in the treatment of RA and other inflammatory diseases.展开更多
Objective:To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus.Methods:Monoarthritis was induced by an injection of 0.3 mL zymosan A(0.9%NaCl,v/v)in the right posteri...Objective:To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus.Methods:Monoarthritis was induced by an injection of 0.3 mL zymosan A(0.9%NaCl,v/v)in the right posterior knee joints of rats.Then,joint diameter and pain threshold were determined.Polyarthritis was induced by an intracaudal injection of complete Freund’s adjuvant and rats were treated from day 14 post 1st complete Freund’s adjuvant injection until 28 day.The clinical,hematological,biochemical and oxidative stress parameters were evaluated.In addition,histological analysis of the knee joint was perfomed in both tests.Results:The aqueous and methanolic extracts of Distemonanthus benthamianus at a dose of 500 mg/kg ameliorated zymosan A-induced monoarthritis,as evidenced by reduced joint diameter,increased pain threshold,as well as improved joint architecture.In addition,both extracts of Distemonanthus benthamianus markedly increased body weight and pain threshold,while reducing paw edema in polyarthritic rats.They also led to a marked decrease in platelets and white blood cells(P<0.05),as well as a significant increase in red blood cells,hemoglobin and hematocrit(P<0.05).The aqueous and methanolic extracts of Distemonanthus benthamianus significantly reduced alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase activities,while increasing serum protein levels(P<0.05)with no significant variation in creatinine level.Moreover,both extracts increased catalase and glutathione activities(P<0.05),and inhibited malondialdehyde and nitric oxide production(P<0.01 and P<0.001)in the liver and kidneys.Histological analysis of the joints showed that both extracts triggered tissue reparation.Conclusions:Distemonanthus benthamianus could be used as a potential candidate in the treatment of rheumatoid arthritis.展开更多
基金supported by the Project in the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(No.2012BAI29B07)National Natural Science Foundation of China(No.81202898)
文摘Rheumatoid arthritis （RA） is the most common inflammatory arthritis and a major cause of disability. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to severe adverse effects or ineffectiveness. The Guge Fengtong formula （GGFT）, containing the standardized extracts of Dioscoreae Nipponicae Rhizoma, Spatholobi Caulis, and Zingiberis Rhizoma, has long been used for RA treatment in China. However, the detailed anti-inflammatory and anti-arthritic activity of GGFT has not been reported so far. In the present work, we aimed to evaluate the anti-inflammatory and anti-arthritic effects of GGFT using three in vivo animal models and tried to uncover the underlying mechanism of action in RAW 264.7 macrophages. The obtained results indicated that GGFT significantly attenuated ear edema, decreased carrageenan-induced paw edema, reduced the ar- thritis score, and reversed the weight loss of the complete Freund＇s adjuvant （CFA）-injected rats. Additionally, decrease in synovial inflammatory infiltration and synovial lining hyperplasia in the joints and decline of inflammatory factors （TNF-α and IL-1β） in the serum were observed in the GGFT-treated rats. In lipopolysaccharide-activated RAW264.7 macrophages, GGFT reduced the produc- tion of NO, PGE2, and IL-6 and inhibited the expression of iNOS, COX-2, and NF-kB. Our results demonstrated that GGFT possessed considerable anti-inflammatory activity and had potential therapeutic effects on adjuvant induced arthritis in rats, providing experi- mental evidences for its application in the treatment of RA and other inflammatory diseases.
文摘Objective:To evaluate the anti-arthritic effect of aqueous and methanolic extracts of Distemonanthus benthamianus.Methods:Monoarthritis was induced by an injection of 0.3 mL zymosan A(0.9%NaCl,v/v)in the right posterior knee joints of rats.Then,joint diameter and pain threshold were determined.Polyarthritis was induced by an intracaudal injection of complete Freund’s adjuvant and rats were treated from day 14 post 1st complete Freund’s adjuvant injection until 28 day.The clinical,hematological,biochemical and oxidative stress parameters were evaluated.In addition,histological analysis of the knee joint was perfomed in both tests.Results:The aqueous and methanolic extracts of Distemonanthus benthamianus at a dose of 500 mg/kg ameliorated zymosan A-induced monoarthritis,as evidenced by reduced joint diameter,increased pain threshold,as well as improved joint architecture.In addition,both extracts of Distemonanthus benthamianus markedly increased body weight and pain threshold,while reducing paw edema in polyarthritic rats.They also led to a marked decrease in platelets and white blood cells(P<0.05),as well as a significant increase in red blood cells,hemoglobin and hematocrit(P<0.05).The aqueous and methanolic extracts of Distemonanthus benthamianus significantly reduced alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase activities,while increasing serum protein levels(P<0.05)with no significant variation in creatinine level.Moreover,both extracts increased catalase and glutathione activities(P<0.05),and inhibited malondialdehyde and nitric oxide production(P<0.01 and P<0.001)in the liver and kidneys.Histological analysis of the joints showed that both extracts triggered tissue reparation.Conclusions:Distemonanthus benthamianus could be used as a potential candidate in the treatment of rheumatoid arthritis.
