美洲大蠊(Periplaneta americana L.)是传统的中药材,有活血散瘀、解毒消疳的功效,具有多种活性成分和广泛的药理作用,临床上常用于金创、外伤、烧伤、烫伤、溃疡、褥疮之创面及胃痛出血等疾病。近年来,美洲大蠊在消化系统疾病方面广泛...美洲大蠊(Periplaneta americana L.)是传统的中药材,有活血散瘀、解毒消疳的功效,具有多种活性成分和广泛的药理作用,临床上常用于金创、外伤、烧伤、烫伤、溃疡、褥疮之创面及胃痛出血等疾病。近年来,美洲大蠊在消化系统疾病方面广泛应用,疗效显著。本文对近年来有关美洲大蠊的化学成分、防治消化系统疾病的药理作用及临床作用进行综述,为美洲大蠊药材的进一步研究和开发提供参考。展开更多
目的鉴定美洲大蠊过敏原Per a 7表达,研究其对辅助性T细胞17(Th17细胞)相关基因和蛋白表达的影响。方法用含编码Per a 7基因的大肠杆菌诱导表达并纯化出Per a 7蛋白。将磁珠分离后CD4+T淋巴细胞与Per a 7激发活化的树突状细胞共培养。...目的鉴定美洲大蠊过敏原Per a 7表达,研究其对辅助性T细胞17(Th17细胞)相关基因和蛋白表达的影响。方法用含编码Per a 7基因的大肠杆菌诱导表达并纯化出Per a 7蛋白。将磁珠分离后CD4+T淋巴细胞与Per a 7激发活化的树突状细胞共培养。采用实时荧光定量PCR检测IL-17、RORγt和Foxp3mRNA表达,ELISA检测上清液中IL-17含量。结果成功克隆分子量约为33kD的重组蛋白Per a 7。重组Per a 7激发后树突状细胞与CD4+T淋巴细胞共培养后,IL-17、RORγt mRNA表达以及上清液中IL-17含量增加(P<0.05),而Foxp3mRNA表达无明显变化(P>0.05)。结论美洲大蠊过敏原Per a 7能激发树突状细胞诱导T淋巴细胞向Th17细胞方向分化,在过敏性疾病发生中起了重要的作用。展开更多
Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthe...Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthenogenesis in which female offspring are produced without fertilization. However, the cytological and genetic mecha? nisms of parthenogenesis in cockroaches are not well understood. Here we provide the first molecular genetic evidence that cockroaches can reproduce through automixis. Using the American cockroach Periplaneta aniericana, we performed microsatellite analysis to investigate the genetic relationship between parthenogenetically produced nymphs and the parent virgin females, and found that all parthenogenetic offspring were homozygous for autosomal microsatellite markers, whereas the female parents were heterozygous. In addition, flow cytometry analysis revealed that the parthenogenetic offspring were diploid. Taken together, our results demonstrate that P. americana exhibits automixis-type thelytoky, in which diploidy is restored by gamete duplication or terminal fusion. These findings highlight the unique reproduction strategies of cockroaches, which are more varied than was previously recognized.展开更多
文摘美洲大蠊(Periplaneta americana L.)是传统的中药材,有活血散瘀、解毒消疳的功效,具有多种活性成分和广泛的药理作用,临床上常用于金创、外伤、烧伤、烫伤、溃疡、褥疮之创面及胃痛出血等疾病。近年来,美洲大蠊在消化系统疾病方面广泛应用,疗效显著。本文对近年来有关美洲大蠊的化学成分、防治消化系统疾病的药理作用及临床作用进行综述,为美洲大蠊药材的进一步研究和开发提供参考。
基金Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China(20093401110006)the Natural Science Foundation of the Department of Education of Anhui Province(KJ2010A026)+1 种基金the Introduction Project of Academic and Technology Leaders in Anhui University(32030066)the Innovative Research Team Program of 211 Project in Anhui University~~
文摘目的鉴定美洲大蠊过敏原Per a 7表达,研究其对辅助性T细胞17(Th17细胞)相关基因和蛋白表达的影响。方法用含编码Per a 7基因的大肠杆菌诱导表达并纯化出Per a 7蛋白。将磁珠分离后CD4+T淋巴细胞与Per a 7激发活化的树突状细胞共培养。采用实时荧光定量PCR检测IL-17、RORγt和Foxp3mRNA表达,ELISA检测上清液中IL-17含量。结果成功克隆分子量约为33kD的重组蛋白Per a 7。重组Per a 7激发后树突状细胞与CD4+T淋巴细胞共培养后,IL-17、RORγt mRNA表达以及上清液中IL-17含量增加(P<0.05),而Foxp3mRNA表达无明显变化(P>0.05)。结论美洲大蠊过敏原Per a 7能激发树突状细胞诱导T淋巴细胞向Th17细胞方向分化,在过敏性疾病发生中起了重要的作用。
文摘Parthenogenesis is an asexual mode of reproduction that plays an important role in the evolution of sex, sociality, and reproduction strategies in insects. Some species of cockroach exhibit thelytoky, a type of parthenogenesis in which female offspring are produced without fertilization. However, the cytological and genetic mecha? nisms of parthenogenesis in cockroaches are not well understood. Here we provide the first molecular genetic evidence that cockroaches can reproduce through automixis. Using the American cockroach Periplaneta aniericana, we performed microsatellite analysis to investigate the genetic relationship between parthenogenetically produced nymphs and the parent virgin females, and found that all parthenogenetic offspring were homozygous for autosomal microsatellite markers, whereas the female parents were heterozygous. In addition, flow cytometry analysis revealed that the parthenogenetic offspring were diploid. Taken together, our results demonstrate that P. americana exhibits automixis-type thelytoky, in which diploidy is restored by gamete duplication or terminal fusion. These findings highlight the unique reproduction strategies of cockroaches, which are more varied than was previously recognized.
