There has been a great discrepancy of pathological diagnosis for gastric non-invasive neoplasia/dysplasia between Japanese and western pathologists. In Japan, lesions that most western pathologists diagnose as dysplas...There has been a great discrepancy of pathological diagnosis for gastric non-invasive neoplasia/dysplasia between Japanese and western pathologists. In Japan, lesions that most western pathologists diagnose as dysplasia are often considered adenocarcinoma based on nuclear and structural atypia regardless of the presence of invasion. In the Vienna classification, gastric non-invasive intraepithelial neoplasia(NIN) weredivided into low grade and high grade(including intramucosal cancer of Japanese criteria). The diagnosis by both endoscopy and pathology of biopsy specimen is difficult. Recent advances of diagnostic modality such as magnified endoscopy and imaged enhanced endoscopy is expected to improve the diagnostic yield for NIN. There are two treatment strategies for NIN, observation and diagnostic therapy by endoscopic resection(ER). ER is acceptable because of its less invasiveness and high local control rate, on the other hand, cancer-developing rate of low-grade NIN is reported to be low. Therefore there is controversy for the treatment of gastric NIN. Prospective study based on unified pathological definition is required in the future.展开更多
Dengue virus(DENV) is a mosquito-borne virus belonging to the Flaviviridae family. There are 4 serotypes of DENV that cause human disease through transmission by mosquito vectors. DENV infection results in a broad spe...Dengue virus(DENV) is a mosquito-borne virus belonging to the Flaviviridae family. There are 4 serotypes of DENV that cause human disease through transmission by mosquito vectors. DENV infection results in a broad spectrum of clinical symptoms, ranging from mild fever to dengue hemorrhagic fever(DHF), the latter of which can progress to dengue shock syndrome(DSS) and death. Researchers have made unremitting efforts over the last half-century to understand DHF pathogenesis. DHF is probably caused by multiple factors, such as virus-specific antibodies, viral antigens and host immune responses. This review summarizes the current progress of studies on DHF pathogenesis, which may provide important information for achieving effective control of dengue in the future.展开更多
Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these ...Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D in vitro. Two hun dred and twenty-two men were in eluded in our study. Vitamin D was detected using an electrochemilumi nesce nee method. Spermatozoa used for in vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters in creased after in cubation with 1,25(OH)2D, especially upon in cubation for 30 min with it at a concen tration of 0.1 nmol l-1. Under these in cubation conditions, the upward migratio n of spermatozoa in creased remarkably with increasing ade nosine triphosphate (ATP) con centratio n. The concentrati on of cyclic ade nosine mono phosphate (cAMP) and the activity of protei n kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the in crease of ATP producti on. The conce ntrations of cytoplasmic calcium ions and n icotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be invoIved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the in crease in in tracellular calcium ions.展开更多
A set of novel graph-theoretical parameters, called the atomic distance-edge (ADE) vector, was developed. Baaed on the connecting C-C bond number between central carbon atom and the other ones, various carbon atoms of...A set of novel graph-theoretical parameters, called the atomic distance-edge (ADE) vector, was developed. Baaed on the connecting C-C bond number between central carbon atom and the other ones, various carbon atoms of alkanes were classified as four types, i.e., type 1, 2, 3 and 4 for primary, secondary, ternary and quaternary carbon, respectively; and then four regression equations were obtained to link carbon-13 chemical shift (CS) of each type of atoms. Furthermore, these regression models were used to predict the carbon-13 nuclear magnetic resonance spectra of alkanes and it was found that the estimated CS were in agreement with the experimental results.展开更多
近年来陆续在一些病毒中发现了一种现象,即针对某种病毒的抗体在亚中和滴度水平能明显增强Fc受体或补体受体阳性细胞对此种病毒感染,这种现象称为抗体依赖的病毒感染增强作用(Antibody-dependent Enhancement of virus infection,ADE)。...近年来陆续在一些病毒中发现了一种现象,即针对某种病毒的抗体在亚中和滴度水平能明显增强Fc受体或补体受体阳性细胞对此种病毒感染,这种现象称为抗体依赖的病毒感染增强作用(Antibody-dependent Enhancement of virus infection,ADE)。ADE存在两种形式:(1)Fc受体介导的抗体依赖的病毒感染增强作用;(2)补体介导的抗体依赖的病毒感染增强作用。ADE有着重要的生物学意义,国外进行了许多体外和一些体内实验研究,本文综述了近几年这方面的有关讲展。展开更多
文摘There has been a great discrepancy of pathological diagnosis for gastric non-invasive neoplasia/dysplasia between Japanese and western pathologists. In Japan, lesions that most western pathologists diagnose as dysplasia are often considered adenocarcinoma based on nuclear and structural atypia regardless of the presence of invasion. In the Vienna classification, gastric non-invasive intraepithelial neoplasia(NIN) weredivided into low grade and high grade(including intramucosal cancer of Japanese criteria). The diagnosis by both endoscopy and pathology of biopsy specimen is difficult. Recent advances of diagnostic modality such as magnified endoscopy and imaged enhanced endoscopy is expected to improve the diagnostic yield for NIN. There are two treatment strategies for NIN, observation and diagnostic therapy by endoscopic resection(ER). ER is acceptable because of its less invasiveness and high local control rate, on the other hand, cancer-developing rate of low-grade NIN is reported to be low. Therefore there is controversy for the treatment of gastric NIN. Prospective study based on unified pathological definition is required in the future.
文摘Dengue virus(DENV) is a mosquito-borne virus belonging to the Flaviviridae family. There are 4 serotypes of DENV that cause human disease through transmission by mosquito vectors. DENV infection results in a broad spectrum of clinical symptoms, ranging from mild fever to dengue hemorrhagic fever(DHF), the latter of which can progress to dengue shock syndrome(DSS) and death. Researchers have made unremitting efforts over the last half-century to understand DHF pathogenesis. DHF is probably caused by multiple factors, such as virus-specific antibodies, viral antigens and host immune responses. This review summarizes the current progress of studies on DHF pathogenesis, which may provide important information for achieving effective control of dengue in the future.
基金the Research Funds for Jiangsu Province Key Research and Development Plan (Grant No. BE2016750)Research Funds for Military Family Planning (Grant No. 16JS012)+5 种基金Six Talent Peaks Project in Jiangsu Province (Grant No. 2017-WSW-033)Chinese Medical Association Special Fund for Clinical Medical Research (Grant No. 17020350704)Foundation for Key Medical Talents in Jiangsu Province (Grant No. ZDRCA2016096)Natural Science Foundation of Jiangsu Province (Grant No. BK20170620)China Postdoctoral Science Foundation (Grant No. 2017M613434)National Natural Science Foundation of China (Grant No. 81701431 and 81701440).
文摘Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D in vitro. Two hun dred and twenty-two men were in eluded in our study. Vitamin D was detected using an electrochemilumi nesce nee method. Spermatozoa used for in vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters in creased after in cubation with 1,25(OH)2D, especially upon in cubation for 30 min with it at a concen tration of 0.1 nmol l-1. Under these in cubation conditions, the upward migratio n of spermatozoa in creased remarkably with increasing ade nosine triphosphate (ATP) con centratio n. The concentrati on of cyclic ade nosine mono phosphate (cAMP) and the activity of protei n kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the in crease of ATP producti on. The conce ntrations of cytoplasmic calcium ions and n icotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be invoIved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the in crease in in tracellular calcium ions.
文摘A set of novel graph-theoretical parameters, called the atomic distance-edge (ADE) vector, was developed. Baaed on the connecting C-C bond number between central carbon atom and the other ones, various carbon atoms of alkanes were classified as four types, i.e., type 1, 2, 3 and 4 for primary, secondary, ternary and quaternary carbon, respectively; and then four regression equations were obtained to link carbon-13 chemical shift (CS) of each type of atoms. Furthermore, these regression models were used to predict the carbon-13 nuclear magnetic resonance spectra of alkanes and it was found that the estimated CS were in agreement with the experimental results.
文摘近年来陆续在一些病毒中发现了一种现象,即针对某种病毒的抗体在亚中和滴度水平能明显增强Fc受体或补体受体阳性细胞对此种病毒感染,这种现象称为抗体依赖的病毒感染增强作用(Antibody-dependent Enhancement of virus infection,ADE)。ADE存在两种形式:(1)Fc受体介导的抗体依赖的病毒感染增强作用;(2)补体介导的抗体依赖的病毒感染增强作用。ADE有着重要的生物学意义,国外进行了许多体外和一些体内实验研究,本文综述了近几年这方面的有关讲展。
