A ultra performance liquid chromatography-electrospray ionization mass spectrometry(UPLC-ESI MS)method was developed for the analysis of saponins in crude extracts from Acanthopanax senticosus Harms.The analysis was p...A ultra performance liquid chromatography-electrospray ionization mass spectrometry(UPLC-ESI MS)method was developed for the analysis of saponins in crude extracts from Acanthopanax senticosus Harms.The analysis was performed on a reversed-phase C18 column using a binary eluent(methanol and 0.2% aqueous HCOOH) under gradient conditions.Three major peaks were detected in the negative ion mode.The molecular -ions of saponins were observed,and the relative molecular mass were obtained.The structure of the ginsenosides was identified by a collision induced dissociation(CID) experiment.The UPLC-ESI MS method was particularly useful in the identification of some saponins.展开更多
文摘A ultra performance liquid chromatography-electrospray ionization mass spectrometry(UPLC-ESI MS)method was developed for the analysis of saponins in crude extracts from Acanthopanax senticosus Harms.The analysis was performed on a reversed-phase C18 column using a binary eluent(methanol and 0.2% aqueous HCOOH) under gradient conditions.Three major peaks were detected in the negative ion mode.The molecular -ions of saponins were observed,and the relative molecular mass were obtained.The structure of the ginsenosides was identified by a collision induced dissociation(CID) experiment.The UPLC-ESI MS method was particularly useful in the identification of some saponins.
