旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表...旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表达量依次为白色>黑色>棕色;mRNA表达量在棕色和白色、棕色和黑色山羊皮肤之间均存在显著差异(P<0.05),在白色和黑色山羊皮肤之间差异不显著(P>0.05)。ASIP在白色、黑色、棕色山羊皮肤组织中的表达量:白色>棕色>黑色;ASIP表达量在白色和黑色山羊皮肤组织之间存在显著差异(P<0.05),但在白色和棕色、棕色和黑色山羊皮肤组织之间差异均不显著(P>0.05)。研究结果提示,Agouti基因在不同毛色山羊皮肤组织中可能存在不同调控机制。展开更多
The rapid development of multimedia techniques has increased the demands on multimedia processors. This paper presents a new design method to quickly design high performance processors for new multimedia applications....The rapid development of multimedia techniques has increased the demands on multimedia processors. This paper presents a new design method to quickly design high performance processors for new multimedia applications. In this approach, a configurable processor based on the very long instruction-set word architecture is used as the basic core for designers to easily configure new processor cores for multimedia algorithm. Specific instructions designed for multimedia applications efficiently improve the performance of the target processor. Functions not implemented in the digital signal processor （DSP） core can be easily integrated into the target processor as user-defined hardware to increase the performance. Several examples are given based on the architecture. The results show that the processor performance is enhanced approximately 4 times on the H.263 codec and that the processor outperforms both DSPs and single instruction multiple data （SIMD） multimedia extension architectures by up to 8 times when computing the 2-D-IDCT.展开更多
Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell p...Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5’-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,353 aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 52.6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.展开更多
提出了基于PXI(PCI Extensions for Instrumentation)总线神光-III原型激光装置物理诊断控制系统的一个解决方案,重点论述了通过嵌入式片上系统(system-on-chip,SoC)实现高实时性控制子系统,从而建立起可扩展的复杂控制系统。而自行设计...提出了基于PXI(PCI Extensions for Instrumentation)总线神光-III原型激光装置物理诊断控制系统的一个解决方案,重点论述了通过嵌入式片上系统(system-on-chip,SoC)实现高实时性控制子系统,从而建立起可扩展的复杂控制系统。而自行设计的SoC平台又可以根据不同的应用需求灵活扩展控制功能,满足不同时间粒度的实时性要求,提高了控制系统的稳定性和可靠性。展开更多
Motion estimation is the most important module in H.264 video encoding algorithm since it offer the best compression ratio compared to intra prediction and entropy encoding. However, using the allowed features for int...Motion estimation is the most important module in H.264 video encoding algorithm since it offer the best compression ratio compared to intra prediction and entropy encoding. However, using the allowed features for inter prediction such as variable block size matching, multi-reference frames and fractional pel search needs a lot of computation cycles. For this purpose, we propose in this paper an Application Specific Instruction-set Processor (ASIP) solution for implementing inter prediction. An exhaustive full and fractional pel combined with variable block size matching search are used. The solution, implemented in FPGA, offers both performance and flexibility to the user to reconfigure the search algorithm.展开更多
文摘旨在探讨Agouti基因调控山羊毛色机制,采用SYBR Green real-time PCR和蛋白免疫印迹方法分别检测了不同毛色山羊皮肤组织中Agouti基因mRNA及其所编码ASIP蛋白的差异表达。结果表明,Agouti基因mRNA在白色、黑色、棕色山羊皮肤组织中的表达量依次为白色>黑色>棕色;mRNA表达量在棕色和白色、棕色和黑色山羊皮肤之间均存在显著差异(P<0.05),在白色和黑色山羊皮肤之间差异不显著(P>0.05)。ASIP在白色、黑色、棕色山羊皮肤组织中的表达量:白色>棕色>黑色;ASIP表达量在白色和黑色山羊皮肤组织之间存在显著差异(P<0.05),但在白色和棕色、棕色和黑色山羊皮肤组织之间差异均不显著(P>0.05)。研究结果提示,Agouti基因在不同毛色山羊皮肤组织中可能存在不同调控机制。
基金Supported by the National Natural Science Foundation of China (No. 60236020)the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20050003083)
文摘The rapid development of multimedia techniques has increased the demands on multimedia processors. This paper presents a new design method to quickly design high performance processors for new multimedia applications. In this approach, a configurable processor based on the very long instruction-set word architecture is used as the basic core for designers to easily configure new processor cores for multimedia algorithm. Specific instructions designed for multimedia applications efficiently improve the performance of the target processor. Functions not implemented in the digital signal processor （DSP） core can be easily integrated into the target processor as user-defined hardware to increase the performance. Several examples are given based on the architecture. The results show that the processor performance is enhanced approximately 4 times on the H.263 codec and that the processor outperforms both DSPs and single instruction multiple data （SIMD） multimedia extension architectures by up to 8 times when computing the 2-D-IDCT.
基金research grants fromthe Special Grant for Human Genomics Program ofChinese Academy of Sciences and the Special Fundsfor Ma
文摘Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5’-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,353 aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 52.6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.
文摘提出了基于PXI(PCI Extensions for Instrumentation)总线神光-III原型激光装置物理诊断控制系统的一个解决方案,重点论述了通过嵌入式片上系统(system-on-chip,SoC)实现高实时性控制子系统,从而建立起可扩展的复杂控制系统。而自行设计的SoC平台又可以根据不同的应用需求灵活扩展控制功能,满足不同时间粒度的实时性要求,提高了控制系统的稳定性和可靠性。
文摘Motion estimation is the most important module in H.264 video encoding algorithm since it offer the best compression ratio compared to intra prediction and entropy encoding. However, using the allowed features for inter prediction such as variable block size matching, multi-reference frames and fractional pel search needs a lot of computation cycles. For this purpose, we propose in this paper an Application Specific Instruction-set Processor (ASIP) solution for implementing inter prediction. An exhaustive full and fractional pel combined with variable block size matching search are used. The solution, implemented in FPGA, offers both performance and flexibility to the user to reconfigure the search algorithm.
