目的:通过检测不同分组患者血清中乙酰胆碱受体抗体(AchR-Ab)、连接素抗体(Titin antibodies,Titin-Ab)和兰尼碱受体抗体(Ryanodine receptor,RyR-Ab)的表达,探讨以上三种抗体在重症肌无力(myasthenia ggravis,MG)诊断和治疗中的临床意...目的:通过检测不同分组患者血清中乙酰胆碱受体抗体(AchR-Ab)、连接素抗体(Titin antibodies,Titin-Ab)和兰尼碱受体抗体(Ryanodine receptor,RyR-Ab)的表达,探讨以上三种抗体在重症肌无力(myasthenia ggravis,MG)诊断和治疗中的临床意义。方法:应用ELISA法对34例MG合并胸腺病变且接受胸腺完整切除的患者,28例其他神经系统疾病的患者和30例正常健康者的血清内进行AchR抗体、Titin抗体和RyR抗体的检测,并于MG合并胸腺病变患者行胸腺切除术后1周及术后3个月时重复检测。结果:MG合并胸腺病变组患者血清内的AchR抗体、Titin抗体要明显高于其他两对照组(P<0.01),RyR抗体水平亦高于其他两组(P<0.05);伴胸腺瘤的重症肌无力(mg with thymoma,MGT)组患者的Titin抗体、RyR抗体明显高于伴胸腺增生的重症肌无力(mg with thymic hyperplasia,MGH)和伴胸腺囊肿的重症肌无力(mg with thymic cyst,MGC)组患者(P<0.01),AchR抗体对MGT组患者亦有较高的阳性率(P<0.05);胸腺切除治疗后仅MGT组患者的AchR-Ab在第一周即发生明显下降(P<0.01),RyR-Ab在术后3个月时有下降(P<0.05),而其他组MG患者的三种抗体均未发现在胸腺切除前后有何变化(P>0.05)。结论:AchR-Ab、Titin-Ab和RyR-Ab与MG的发病机制有关;三种抗体均有助于对MG患者的诊断,其中Titin-Ab和RyR-Ab可用于胸腺瘤的鉴别诊断,并可作为诊断MGT的主要客观指标;对于MGT经胸腺切除术后患者可通过检测AchR抗体观察早期病情改变,检测RyR-Ab亦可作为其中远期病情改变的客观指标,但这两项指标与患者预后恢复情况的相关性还有待于进一步研究。展开更多
Objective: Seropositive myasthenia gravis (MG) depends on the presence of acetylcholine receptor antibodies (AchR-Ab) against nicotinic acetylcholine receptors at the postsynaptic neuromuscular junction. In this study...Objective: Seropositive myasthenia gravis (MG) depends on the presence of acetylcholine receptor antibodies (AchR-Ab) against nicotinic acetylcholine receptors at the postsynaptic neuromuscular junction. In this study, we investigated the effect of AchR-Ab levels to symptoms and treatment in the MG patients underwent surgery for thymic pathology. Materilas and Methods: The records including level of preoperative AchR-Ab, type of thymic pathology, the changes of symptoms and treatment after surgery for thymic pathology of 37 MG patients between January 2007 and December 2015 have been viewed retrospectively. Results: The mean age of 37 patients (21 females, 16 males) was 40.2 ± 14.9 years (range, 18 to 75 years). The mean of the level of AchR-Ab was 144.7 ± 427.6 nmol/L (range, 0.1 to 1806 nmol/L). In the patient group including the reduced use of anticholinesterase after surgery the mean of level of AchR-Ab was 241.3 nmol/L while it was 10.8 nmol/L in the patient group including the non-reduced use of anticholinesterase (p = 0.082) after a mean follow-up period of 23 months postoperatively. The mean levels were 246.7 nmol/L and 8.5 nmol/L for the reduced and non-reduced use of corticosteroid patient groups, respectively (p = 0.001). In the 25 patients with fewer symptom after surgery the mean of the AchR-Ab level was 205.3 nmol/L while it was 18.3 nmol/L in the patients without any changes (p = 0.071). Conclusion: We concluded that the preoperative level of AchR-Ab was associated with postoperative dosage of anticholinesterase and corticosteroids and severity of symptoms postoperatively. We think that the levels of AchR-Ab titers can be a marker for the efficacy of thymic surgery.展开更多
文摘目的:通过检测不同分组患者血清中乙酰胆碱受体抗体(AchR-Ab)、连接素抗体(Titin antibodies,Titin-Ab)和兰尼碱受体抗体(Ryanodine receptor,RyR-Ab)的表达,探讨以上三种抗体在重症肌无力(myasthenia ggravis,MG)诊断和治疗中的临床意义。方法:应用ELISA法对34例MG合并胸腺病变且接受胸腺完整切除的患者,28例其他神经系统疾病的患者和30例正常健康者的血清内进行AchR抗体、Titin抗体和RyR抗体的检测,并于MG合并胸腺病变患者行胸腺切除术后1周及术后3个月时重复检测。结果:MG合并胸腺病变组患者血清内的AchR抗体、Titin抗体要明显高于其他两对照组(P<0.01),RyR抗体水平亦高于其他两组(P<0.05);伴胸腺瘤的重症肌无力(mg with thymoma,MGT)组患者的Titin抗体、RyR抗体明显高于伴胸腺增生的重症肌无力(mg with thymic hyperplasia,MGH)和伴胸腺囊肿的重症肌无力(mg with thymic cyst,MGC)组患者(P<0.01),AchR抗体对MGT组患者亦有较高的阳性率(P<0.05);胸腺切除治疗后仅MGT组患者的AchR-Ab在第一周即发生明显下降(P<0.01),RyR-Ab在术后3个月时有下降(P<0.05),而其他组MG患者的三种抗体均未发现在胸腺切除前后有何变化(P>0.05)。结论:AchR-Ab、Titin-Ab和RyR-Ab与MG的发病机制有关;三种抗体均有助于对MG患者的诊断,其中Titin-Ab和RyR-Ab可用于胸腺瘤的鉴别诊断,并可作为诊断MGT的主要客观指标;对于MGT经胸腺切除术后患者可通过检测AchR抗体观察早期病情改变,检测RyR-Ab亦可作为其中远期病情改变的客观指标,但这两项指标与患者预后恢复情况的相关性还有待于进一步研究。
文摘Objective: Seropositive myasthenia gravis (MG) depends on the presence of acetylcholine receptor antibodies (AchR-Ab) against nicotinic acetylcholine receptors at the postsynaptic neuromuscular junction. In this study, we investigated the effect of AchR-Ab levels to symptoms and treatment in the MG patients underwent surgery for thymic pathology. Materilas and Methods: The records including level of preoperative AchR-Ab, type of thymic pathology, the changes of symptoms and treatment after surgery for thymic pathology of 37 MG patients between January 2007 and December 2015 have been viewed retrospectively. Results: The mean age of 37 patients (21 females, 16 males) was 40.2 ± 14.9 years (range, 18 to 75 years). The mean of the level of AchR-Ab was 144.7 ± 427.6 nmol/L (range, 0.1 to 1806 nmol/L). In the patient group including the reduced use of anticholinesterase after surgery the mean of level of AchR-Ab was 241.3 nmol/L while it was 10.8 nmol/L in the patient group including the non-reduced use of anticholinesterase (p = 0.082) after a mean follow-up period of 23 months postoperatively. The mean levels were 246.7 nmol/L and 8.5 nmol/L for the reduced and non-reduced use of corticosteroid patient groups, respectively (p = 0.001). In the 25 patients with fewer symptom after surgery the mean of the AchR-Ab level was 205.3 nmol/L while it was 18.3 nmol/L in the patients without any changes (p = 0.071). Conclusion: We concluded that the preoperative level of AchR-Ab was associated with postoperative dosage of anticholinesterase and corticosteroids and severity of symptoms postoperatively. We think that the levels of AchR-Ab titers can be a marker for the efficacy of thymic surgery.
