目的:分析线粒体活性对人类精子活动力的影响。方法:以线粒体特异性荧光染料--mitotracker deep red 633(MTDR对不同活力的精子进行染色,然后用流式细胞术测定精子线粒体活性(即MTDR荧光强度值)。用雷帕霉素(rapamycin,RP)和羟氯喹(hydr...目的:分析线粒体活性对人类精子活动力的影响。方法:以线粒体特异性荧光染料--mitotracker deep red 633(MTDR对不同活力的精子进行染色,然后用流式细胞术测定精子线粒体活性(即MTDR荧光强度值)。用雷帕霉素(rapamycin,RP)和羟氯喹(hydroxychloroquineon,HCQ)调控精子活性水平,测定精子活动力的变化。结果:MTDR荧光强度值与前向运动速率(progressive motility,PR)不相关(r=0.182,P=0.268)。5 nmol/L和100 nmol/L的RP均可明显降低线粒体活性,50μmol/L HCQ可明显提高线粒体活性(P<0.05),但它们对精子活动力影响都不明显(P>0.05)。结论:线粒体活性对人类精子活力影响不明显。展开更多
The experimental scheme of 633 nm and 1359 nm good-bad cavity dual-wavelength active optical frequency stan- dard is proposed, where He-Ne 633nm and Cs 1359nm stimulated emissions are working at good-cavity and bad-ca...The experimental scheme of 633 nm and 1359 nm good-bad cavity dual-wavelength active optical frequency stan- dard is proposed, where He-Ne 633nm and Cs 1359nm stimulated emissions are working at good-cavity and bad-cavity regimes, respectively. The cavity length is stabilized by locking the 633nm output frequency to a super-cavity with the Pound Drever-Hall （PDH） technique. The frequency stability of 1359 nm bad-cavity stim- ulated emission output is then expected to be further improved by at least 1 order of magnitude than the 633nm PDH system due to the suppressed cavity pulling effect of active optical clock, and the quantum limited linewidth of 1359nm output is estimated to be 72.5 mHz.展开更多
Induced loss at 633 nm is tested in Yb3＋∕Al3＋co-doped silica fiber by a core pumped with a 974 nm laser and probed with a 633 nm laser. The fiber is prepared by the modified chemical vapor deposition method combine...Induced loss at 633 nm is tested in Yb3＋∕Al3＋co-doped silica fiber by a core pumped with a 974 nm laser and probed with a 633 nm laser. The fiber is prepared by the modified chemical vapor deposition method combined with solution doping. Different power scales of pump light and probe light are used in the tests. It is found that there is a dynamic equilibrium between photobleaching induced by 633 nm probe light and photodarkening（PD）induced by 974 nm pump light. For the first time to our knowledge, the effect of 633 nm probe laser power on an induced loss test of Yb3＋∕Al3＋co-doped silica fiber is studied quantitatively. It suggests that as long as the633 nm probe light power is less than 0.2 m W, the induced loss is mainly contributed by the PD effect of pumping light, and the deviation of induced loss is less than 5%.展开更多
This paper reports the fermentation techmology of bacterial feed NO.633. A new fermentational technic, which was composed of fermentation medium, optimum pH,temperature, airate flow, pot pressure and fermentation time...This paper reports the fermentation techmology of bacterial feed NO.633. A new fermentational technic, which was composed of fermentation medium, optimum pH,temperature, airate flow, pot pressure and fermentation time, was established.展开更多
文摘目的:分析线粒体活性对人类精子活动力的影响。方法:以线粒体特异性荧光染料--mitotracker deep red 633(MTDR对不同活力的精子进行染色,然后用流式细胞术测定精子线粒体活性(即MTDR荧光强度值)。用雷帕霉素(rapamycin,RP)和羟氯喹(hydroxychloroquineon,HCQ)调控精子活性水平,测定精子活动力的变化。结果:MTDR荧光强度值与前向运动速率(progressive motility,PR)不相关(r=0.182,P=0.268)。5 nmol/L和100 nmol/L的RP均可明显降低线粒体活性,50μmol/L HCQ可明显提高线粒体活性(P<0.05),但它们对精子活动力影响都不明显(P>0.05)。结论:线粒体活性对人类精子活力影响不明显。
基金Supported by the National Natural Science Foundation of China under Grant Nos 10874009,11074011 and 91436210the International Science&Technology Cooperation Program of China under Grant No 2010DFR10900
文摘The experimental scheme of 633 nm and 1359 nm good-bad cavity dual-wavelength active optical frequency stan- dard is proposed, where He-Ne 633nm and Cs 1359nm stimulated emissions are working at good-cavity and bad-cavity regimes, respectively. The cavity length is stabilized by locking the 633nm output frequency to a super-cavity with the Pound Drever-Hall （PDH） technique. The frequency stability of 1359 nm bad-cavity stim- ulated emission output is then expected to be further improved by at least 1 order of magnitude than the 633nm PDH system due to the suppressed cavity pulling effect of active optical clock, and the quantum limited linewidth of 1359nm output is estimated to be 72.5 mHz.
基金supported by the Young Scientists Fund of the National Natural Science Foundation of China(No.61405215)the Youth Innovation Promotion Associationthe Special Program for Advanced Electronic Materials of the Ministry of Science and Technology,China(No.2016YFB0402201)
文摘Induced loss at 633 nm is tested in Yb3＋∕Al3＋co-doped silica fiber by a core pumped with a 974 nm laser and probed with a 633 nm laser. The fiber is prepared by the modified chemical vapor deposition method combined with solution doping. Different power scales of pump light and probe light are used in the tests. It is found that there is a dynamic equilibrium between photobleaching induced by 633 nm probe light and photodarkening（PD）induced by 974 nm pump light. For the first time to our knowledge, the effect of 633 nm probe laser power on an induced loss test of Yb3＋∕Al3＋co-doped silica fiber is studied quantitatively. It suggests that as long as the633 nm probe light power is less than 0.2 m W, the induced loss is mainly contributed by the PD effect of pumping light, and the deviation of induced loss is less than 5%.
文摘This paper reports the fermentation techmology of bacterial feed NO.633. A new fermentational technic, which was composed of fermentation medium, optimum pH,temperature, airate flow, pot pressure and fermentation time, was established.
